MicroRNA-200b inhibits the proliferation of hepatocellular carcinoma by targeting DNA methyltransferase 3a

Abstract

Aberrant microRNA (miRNA or miR) expression has been reported to contribute to the pathogenesis of hepatocellular carcinoma (HCC). However, the involvement of specific miRNAs in HCC remains to be elucidated. The present study aimed to investigate the potential role of miR-200b and the mechanism underlying its function in hepatocarcinogenesis. The results of the present study demonstrated that the expression levels of miR‑200b were significantly reduced in HCC tissue samples, as compared with normal liver (NL) and para‑tumorous (PT) tissue samples. The results also revealed that miR‑200b expression levels in HepG2 cells were significantly decreased compared with those in L02 cells. In addition, western blotting and reverse transcription‑quantitative polymerase chain reaction demonstrated that the expression levels of DNA methyltransferase 3a (DNMT3a), a possible target gene for miR‑200b, were significantly higher in HCC tissue samples, as compared with those in NL and PT tissue samples. Furthermore, the data suggested that DNMT3a was a direct target gene of miR‑200b. Upregulated miR‑200b expression in HepG2 cells led to a decrease in DNMT3a expression levels, and an inhibition of cell proliferation. These results suggested that miR‑200b has an important role in hepatocarcinogenesis and acts by downregulating DNMT3a expression. Thus, miR-200b may be a promising target for HCC treatment.

Cardiac repair in a mouse model of acute myocardial infarction with trophoblast stem cells

“…6C and D). MiR-200b has been reported to suppress cell proliferation in several cell lines22232425. Thus, we propose that miRNA-200b-3p may also be involved in the regulation of the proliferative effect of TSCs.…”

“…Microarray analysis and qRT-PCR revealed the difference in miRNA-200b-3p between TSCs and MSCs. Recent reports indicated that miRNA-200b is highly correlated with cell proliferation222425. Especially, in cardiac cell lines, Yao et al 31.…”

The Expression of DNA Methyltransferase DNMT3a in Classical and Fibrolamellar Hepatocellular Carcinoma

“…Its elevated expression was observed in gastric cancer as well [10]. Its role has also been established in the development of hepatocellular carcinoma (HCC) -inhibition of DNMT3a expression by microRNA miR-200b causes decrease in tumour proliferative activity [11]. On the other hand, the expression of DNMT3a is up-regulated by coexisting hepatitis B virus (HBV) infection [12], which is one of the main risk factor for development of HCC with the significant exception of fibrolamellar variant.…”

Serum miR‐182 is a predictive biomarker for dichotomization of risk of hepatocellular carcinoma in rats

“…MicroRNAs (miRNAs, miRs) are short noncoding RNAs that can modulate the expression of target messenger RNAs (mRNAs). They have been demonstrated to be dysregulated in experimental and human HCC and have emerged as promising disease biomarkers because of their stability and sequence homology between species, especially in mammals . MicroRNAs can be detected in the circulation .…”

“…They have been demonstrated to be dysregulated in experimental and human HCC and have emerged as promising disease biomarkers because of their stability and sequence homology between species, especially in mammals. [8][9][10][11][12][13][14] MicroRNAs can be detected in the circulation. 15,16 Studies have identified a variety of miRNAs that differentiate HCC patients from healthy controls.…”