MicroRNA-204-5p-Mediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas

Gao, Jing; Wang, Ye; Zhao, Xiaowen; Chen, Peng; Xie, Liping

doi:10.1167/iovs.14-15913

Cited by 53 publications

(51 citation statements)

References 29 publications

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“…The samples were stained with primary antibodies (Supplementary Table 2) and subsequently incubated with fluorescein-conjugated secondary antibodies. All stained samples were observed under a microscope as described previously (14).…”

Section: Immunofluorescencementioning

confidence: 99%

“…The homogenates, which contained 10-20 mg protein, were then separated in 15% SDS-polyacrylamide gel and transferred to polyvinyl difluoride membranes. The blots were probed with primary antibodies as described previously (14).…”

Section: Western Blotmentioning

confidence: 99%

“…In our previous study, overexpression of Sirt1 promoted epithelial wound healing in diabetic corneas (13,14). However, the underlying mechanism by which Sirt1 induces diabetic corneal nerve regeneration remains elusive.…”

mentioning

confidence: 94%

“…microRNAs (miRNAs) are small, noncoding RNA molecules found in multicellular eukaryotes and play essential regulatory roles in translational repression (15). Several wound healing-related miRNAs, such as miR-146a, were identified in the corneas of people with diabetes (14,16,17). Abnormal miR-146a upregulation may be an important mechanism of delayed wound healing in the diabetic cornea (17,18).…”

mentioning

confidence: 99%

“…Sirt1 is a direct target of miR-204-5p in regulating the epithelial cell cycle in the corneas of persons with diabetes, suggesting that Sirt1 is involved in the miRNA-mRNA regulatory network (14), whereas the downstream miRNA targets of Sirt1 in corneal nerve regeneration in diabetes remain unclear.…”

mentioning

confidence: 99%

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microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration

Wang

Zhao

et al. 2016

Diabetes

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Sensory neurons are particularly susceptible to neuronal damage in diabetes, and silent mating type information regulation 2 homolog 1 (Sirt1) has been recently identified as a key gene in neuroprotection and wound healing. We found that the expression of Sirt1 was downregulated in trigeminal sensory neurons of diabetic mice. A microRNA microarray analysis identified microRNA-182 (miR-182) as a Sirt1 downstream effector, and the expression level of miR-182 was increased by Sirt1 overexpression in trigeminal neurons; Sirt1 bound to the promoter of miR-182 and regulated its transcription. We also revealed that miR-182 enhanced neurite outgrowth in isolated trigeminal sensory neurons and overcame the detrimental effects of hyperglycemia by stimulating corneal nerve regeneration by decreasing the expression of one of its target genes, NOX4. Furthermore, the effects of miR-182 on corneal nerve regeneration are associated with a functional recovery of corneal sensation in hyperglycemic conditions. These data demonstrate that miR-182 is a key regulator in diabetic corneal nerve regeneration through targeting NOX4, suggesting that miR-182 might be a potential target for the treatment of diabetic sensory nerve regeneration and diabetic keratopathy.

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Section: Immunofluorescencementioning

confidence: 99%

Section: Western Blotmentioning

confidence: 99%

mentioning

confidence: 94%

mentioning

confidence: 99%

mentioning

confidence: 99%

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microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration

Wang

Zhao

et al. 2016

Diabetes

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Striving for Perfect Vision: Insights from Refractive Surgery

Farooq

Giri

Azar

2019

Foundations of Corneal Disease

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Mechanistic Role of MicroRNAs in Coupling Lipid Metabolism and Atherosclerosis

Novák

Olejníčková

Tkáčová

et al. 2015

Advances in Experimental Medicine and Biology

104

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MicroRNAs (miRNAs, miRs) represent a group of powerful and versatile posttranscriptional regulators of gene expression being involved in the fine control of a plethora of physiological and pathological processes. Besides their well-established crucial roles in the regulation of cell cycle, embryogenesis or tumorigenesis, these tiny molecules have also been shown to participate in the regulation of lipid metabolism. In particular, miRs orchestrate cholesterol and fatty acids synthesis, transport, and degradation and low-density and high-density lipoprotein (LDL and HDL) formation. It is thus not surprising that they have also been reported to affect the development and progression of several lipid metabolism-related disorders including liver steatosis and atherosclerosis. Mounting evidence suggests that miRs might represent important “posttranscriptional hubs” of lipid metabolism, which means that one miR usually targets 3′-untranslated regions of various mRNAs that are involved in different steps of one precise metabolic/signaling pathway, e.g., one miR targets mRNAs of enzymes important for cholesterol synthesis, degradation, and transport. Therefore, changes in the levels of one key miR affect various steps of one pathway, which is thereby promoted or inhibited. This makes miRs potent future diagnostic and even therapeutic tools for personalized medicine. Within this chapter, the most prominent microRNAs involved in lipid metabolism, e.g., miR-27a/b, miR-33/33*, miR-122, miR-144, or miR-223, and their intracellular and extracellular functions will be extensively discussed, in particular focusing on their mechanistic role in the pathophysiology of atherosclerosis. Special emphasis will be given on miR-122, the first microRNA currently in clinical trials for the treatment of hepatitis C and on miR-223, the most abundant miR in lipoprotein particles.

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MicroRNA-204-5p-Mediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas

Abstract: Our data provide firm evidence of a role for miR-204-5p in the direct regulation of SIRT1 in diabetic corneas and identified the miR-204-5p-mediated regulation of SIRT1 contributes to the delay of epithelial cell cycle traversal in diabetic keratopathy. : Chinese Abstract.

Cited by 53 publications

References 29 publications

microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration

microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration

Striving for Perfect Vision: Insights from Refractive Surgery

Mechanistic Role of MicroRNAs in Coupling Lipid Metabolism and Atherosclerosis

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