MicroRNA-21 is involved in oocyte maturation, blastocyst formation, and pre-implantation embryo development

Dehghan, Zeinab; Mohammadi‐Yeganeh, Samira; Rezaee, Delsuz; Salehi, Mohammad

doi:10.1016/j.ydbio.2021.08.008

Cited by 28 publications

(27 citation statements)

References 52 publications

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“…GC miRNAs work together to govern meiotic progression via regulating proliferation, steroidogenesis, and apoptosis, all of which help to promote oocyte maturation [ 8 ]. They have also been linked to disorders, such as infertility, PCOS, EDC exposure, and numerous malignancies [ 10 , 19 , 27 ]. BPA, a widespread EDC, is ubiquitous in the environment and in bodily tissues with endless routes of exposure [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…This increase persisted until 24 h of exposure, mimicking the effects that would be observed in GCs over the course of oocyte maturation as this process takes 24 h to happen in the bovine species [ 66 ]. High levels of miR-21 reduced GC expansion, decreased meiotic progression, inhibited antioxidants expression, and altered gene expression [ 10 ]. Carcinogenic effects of BPA can be supported by its ability to disrupt miRNAs expression.…”

Section: Discussionmentioning

confidence: 99%

“…Oocytes communicate with GCs which produce small molecules that get shuttled back into the oocyte [ 9 ]. miRNAs travel from GCs to the oocyte through gap junctions [ 10 ], making it imperative to explore miRNAs in granulosa cells to understand their role in oocyte maturation and embryo development.…”

Section: Introductionmentioning

confidence: 99%

“…miR-21 expression significantly increases in oocytes during maturation [ 12 , 15 ] and one suggested explanation is the transport of miR-21 from the GCs into the oocyte [ 15 ]. This miRNA has multiple important functions within bovine and human GCs including, but not limited to, cell cycle progression, proliferation, differentiation, migration, and inflammation, and is largely acknowledged as an inhibitor of apoptosis and an oncomiR implicated in cancers [ 10 , 16 , 17 ]. Two main targets of miR-21 are programmed cell death 4 (PDCD4) and phosphatase tensin homolog (PTEN), which induce apoptosis and repress tumor progression, respectively [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition

Sabry

Williams

Werry

et al. 2022

IJMS

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microRNAs (miRNAs) are susceptible to environmental factors that might affect cellular function and impose negative effects on female reproduction. miR-21 is the most abundant miRNA in bovine granulosa cells and is widely reported as affected by Bisphenol A (BPA) exposure, yet the cause and consequences are not entirely elucidated. BPA is a synthetic endocrine disruptor associated with poor fertility. miR-21 function in bovine granulosa cells is investigated utilizing locked nucleic acid (LNA) oligonucleotides to suppress miR-21. Before measuring apoptosis and quantifying miR-21 apoptotic targets PDCD4 and PTEN, transfection was optimized and validated. BPA was introduced to see how it affects miR-21 regulation and which BPA-mediated effects are influenced by miR-21. miR-21 knockdown and specificity against additional miRNAs were confirmed. miR-21 was found to have antiapoptotic effects, which could be explained by its effect on the proapoptotic target PDCD4, but not PTEN. Previous findings of miR-21 overexpression were validated using BPA treatments, and the temporal influence of BPA on miR-21 levels was addressed. Finally, BPA effects on upstream regulators, such as VMP1 and STAT3, explain the BPA-dependent upregulation of miR-21 expression. Overall, this research enhances our understanding of miR-21 function in granulosa cells and the mechanisms of BPA-induced reproductive impairment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition

Sabry

Williams

Werry

et al. 2022

IJMS

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“…In the present study, levels of NAM in FF were significantly correlated with follicular development after induction of ovulation in IVF women, with higher concentrations of NAM tending to result in the development of larger follicles. Oocyte maturation and quality are critical to female fertility, where regulatory factors, such as some genes [ 42 ], proteins [ 43 ] and metabolites [ 44 ], have always been the focus of research in the field of assisted reproduction. Although a number of previous studies have pointed out that NAM can inhibit oocyte maturation and embryonic development [ 26 , 45 ], increasing numbers of studies have confirmed that NAM at an appropriate concentration enhances oocyte quality and even the outcomes of pregnancy.…”

Section: Discussionmentioning

confidence: 99%

Effects of nicotinamide on follicular development and the quality of oocytes

Yang

Feng

et al. 2022

Reprod Biol Endocrinol

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Background Nicotinamide (NAM) is an important antioxidant, which is closely related to female fertility, but its role has not been clearly elucidated. The purpose of the present study was to investigate the effects of NAM on follicular development at different stages and the quality of oocytes. Methods The concentration of NAM in follicular fluid (FF) of 236 women undergoing in vitro fertilization (IVF) was ascertained by enzyme-linked immunosorbent assay (ELISA), and the correlation between NAM and clinical indexes was analyzed. During the in vitro maturation (IVM) of mice cumulus-oocyte complexes (COCs), different concentrations of NAM were added to check the maturation rate and fertilization rate. The reactive oxygen species (ROS) levels in the oocytes treated with different hydrogen peroxide (H2O2) and NAM were assessed. Immunofluorescence staining was performed to measure the proportion of abnormal spindles. Results The level of NAM in large follicles was significantly higher than that in small follicles. In mature FF, the NAM concentration was positively correlated with the rates of oocyte maturation and fertilization. Five mM NAM treatment during IVM increased maturation rate and fertilization rate in the oxidative stress model, and significantly reduced the increase of ROS levels induced by H2O2 in mice oocytes. Conclusions Higher levels of NAM in FF are associated with larger follicle development. The supplement of 5 mM NAM during IVM may improve mice oocyte quality, reducing damage caused by oxidative stress.

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MicroRNAs in POI, DOR and POR

Luo

Sun

2023

Arch Gynecol Obstet

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MicroRNA-21 is involved in oocyte maturation, blastocyst formation, and pre-implantation embryo development

Cited by 28 publications

References 52 publications

BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition

BPA Decreases PDCD4 in Bovine Granulosa Cells Independently of miR-21 Inhibition

Effects of nicotinamide on follicular development and the quality of oocytes

MicroRNAs in POI, DOR and POR

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