MicroRNA-21 regulates breast cancer invasion partly by targeting tissue inhibitor of metalloproteinase 3 expression

Song, Bao-Liang; Wang, Chuanxi; Liu, Jie; Wang, Xin; Lv, Li; Wei, Ling; Xie, Li; Zheng, Yan; Song, Xianrang

doi:10.1186/1756-9966-29-29

Cited by 225 publications

(137 citation statements)

References 27 publications

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“…Previous studies using clinical specimens and in vitro and in vivo models have identified a limited number of miRNAs that promote metastasis, including miR-200, miR-10b and miR-21 (30)(31)(32)(33). The present study revealed that miR-520e is associated with metastasis; upregulation of miR-520e may facilitate the metastasis of breast cancer cells and therefore the progression of breast cancer.…”

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confidence: 49%

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

Long

et al. 2016

Oncology Letters

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Abstract. Previous studies have indicated that the deregulation of microRNAs contributes to tumorigenesis. Misregulation of microRNA-520e (miR-520e) has been observed in various types of cancer. However, the expression profile and biological function of miR-520e in breast cancer remains largely unknown. The present study demonstrated that miR-520e expression was significantly increased in breast cancer tissues compared with adjacent non-cancerous breast tissues in 21 patients, as revealed by reverse transcription-quantitative polymerase chain reaction. Furthermore, the proliferation capacity of breast cancer cells was markedly enhanced by the introduction of miR-520e in vitro using a cell counting kit-8 assay. The present study also revealed that the overexpression of miR-520e could suppress breast cancer cell apoptosis, revealed using Annexin V/propidium iodide double staining and flow cytometry analysis. In addition, the ectopic expression of miR-520e promoted the migration of breast cancer cells in vitro, as demonstrated by a Transwell assay. Overall, the findings of the present study highlight an important role for miR-520e in breast cancer development and in the molecular etiology of breast cancer, which indicates the potential application of miR-520e in cancer therapy. IntroductionPrevious studies have demonstrated that alterations in protein-coding genes are critical for the development of human cancer (1,2). Other studies have indicated that deregulation of non-coding genes, particularly microRNAs (miRNAs), also contribute to tumorigenesis (3-6).miRNAs belong to a class of phylogenetically conserved non-coding RNAs that regulate an abundance of cellular processes by binding to specific target messenger RNAs (7). Numerous studies indicate that miRNAs are important in various biological processes, including the cell cycle, development, cell proliferation, differentiation and apoptosis (8-12). More than half of the miRNA genes are located in chromosomes that have been observed to become amplified, deleted or translocated during the development of cancer (13). In addition, deregulation of specific miRNAs occurs in certain cancer types (14,15), and this has been suggested to be associated with the prognosis of cancer patients (16). Furthermore, a previous study proposed that miRNAs may act as oncogenes or tumor suppressor genes (6). These findings highlight the importance of miRNAs in cancer development and provide insight into the molecular mechanisms that cause tumorigenesis.Globally, breast cancer is the most common cause of cancer-associated mortality in women, and accounted for ~1.38 million novel cases and 458,000 mortalities in 2008 (17). As with other solid tumors, multiple genetic and epigenetic alterations in protein-coding genes have been observed in breast cancer (18). However, previous findings alone cannot explain the complexity of breast cancer. Previous studies have demonstrated that dysfunction of miRNAs contributes to breast cancer development (11,14). Zhang et al reported that microRNA-520e...

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confidence: 49%

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

Long

et al. 2016

Oncology Letters

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“…It was recently demonstrated that microRNAs can promote tumor invasion and metastasis in breast cancer, suggesting that the dysregulation of specific miRNAs can provide a selective advantage in breast cancer metastasis. Various miRNAs serve as metastasis activators [miR-10b (33), miR-21 (34,35), miR-9 (36), miR-373 and miR-520c (37)] and are able to initiate invasion and metastasis in breast cancer. Some miRNAs serve as metastasis suppressors [miR-3 (38), miR-200 family (39,40), miR-335 and miR-126 (41)] and are able to inhibit invasion and metastasis in breast cancer.…”

Section: Discussionmentioning

confidence: 99%

microRNA-320a inhibits tumor invasion by targeting neuropilinï¿½1 and is associated with liver metastasis in colorectal cancer

Zhang

Liu

et al. 2011

Oncol Rep

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Abstract. MicroRNAs (miRNAs) have been implicated in regulating diverse cellular pathways. Although there is emerging evidence that various miRNAs function as oncogenes or tumor suppressors in colorectal cancer (CRC), the role of miRNAs in mediating liver metastasis remains unexplored. The expression profile of miRNAs in liver metastasis and primary CRC tissues was analyzed by miRNA microarrays and verified by real-time polymerase chain reaction (PCR). In 62 CRC patients, the expression levels of miR-320a were determined by real-time PCR, and the effects on migration and invasion of miR-320a were determined using a transwell assay. miR-320a target genes were confirmed by luciferase assay, real-time PCR and Western blot analysis. A set of miRNAs was found to be dysregulated in the liver metastasis tissues compared to matched primary CRC tissues, and the expression levels of miR-320a were significantly decreased in the liver metastasis tissues examined. miR-320a was correlated with tumor progression in CRC. miR-320a was downregulated in liver metastatic colon cancer cells and inhibited liver metastatic colon cancer cell migration and invasion. miR-320a directly binds to the 3'UTR of neuropilin 1 (NRP-1), a protein that functions as a co-receptor of vascular epithelial growth factor. miR-320a downregulated the expression of NRP-1 at both the mRNA and protein levels. These data demonstrated that miR-320a may be useful for identifying CRC patients that are at an elevated risk for developing liver metastasis. Our findings suggest that miR-320a may be a novel therapeutic candidate for the treatment of colorectal cancer.

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“…In particular, we studied the expression of: (a) miR-21, which affects tumor invasion and inhibits tumor cell colonization (17)(18)(19)(20); (b) miR-205, which is involved in angiogenesis and is downregulated in epithelial-mesenchymal transitions (21 ); (c) miR-10b, which positively regulates cell migration and invasion (13 ); (d) miR-335, which modifies tumor microenvironment (12 ); (e) let-7a, which inhibits the proliferation and self-renewal of breast cancer stem cells (22 ); and (f) miR-210, which has been shown to be inversely associated with cancer aggressiveness and metastatic potential (23 ). We explored the relationships of the expression profiles for these miRNAs with clinical outcomes in FFPE samples from breast cancer patients, focusing in particular on ER and PR expression, HER2, and lymph node status.…”

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confidence: 99%

Prognostic Significance of Metastasis-Related MicroRNAs in Early Breast Cancer Patients with a Long Follow-up

et al. 2014

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BACKGROUND: Stability of microRNAs (miRNAs) in formalin-fixed paraffin-embedded (FFPE) tissues enables their reliable analysis in archived FFPE tissue samples, which are an invaluable source for the evaluation of novel biomarkers. Especially in breast cancer, for which late relapses occur in many cases, analysis of miRNAs in FFPE tissues holds great potential, because it can lead to the discovery of novel biomarkers suitable for future routine clinical diagnostics for breast cancer. We investigated the prognostic significance of 6 metastasis-related miRNAs that can critically regulate various stages of migration and invasion and play critical roles in the multistep metastatic process.

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MicroRNA-21 regulates breast cancer invasion partly by targeting tissue inhibitor of metalloproteinase 3 expression

Cited by 225 publications

References 27 publications

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

microRNA-320a inhibits tumor invasion by targeting neuropilinï¿½1 and is associated with liver metastasis in colorectal cancer

Prognostic Significance of Metastasis-Related MicroRNAs in Early Breast Cancer Patients with a Long Follow-up

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