MicroRNA-214-3p Targeting Ctnnb1 Promotes 3T3-L1 Preadipocyte Differentiation by Interfering with the Wnt/β-Catenin Signaling Pathway

Xi, Fengxue; Wei, Chunhong; Xu, Yanting; Ma, Lu; He, Yuelin; Shi, Xin’e; Yang, Gongshe; Yu, Taiyong

doi:10.3390/ijms20081816

Cited by 38 publications

(28 citation statements)

References 43 publications

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“…MiR‑214 was demonstrated to directly interact with the 3'‑UTR of the β‑catenin gene CTNNB1 during osteogenic differentiation of periodontal ligament stem (PDLSC) [ 44 ]. And miR-214 was also demonstrated to target CTNNB1 in human cervical and colorectal cancer cells [ 45 ] and 3T3-L1 pre-adipocyte [ 46 ]. Our result primarily verified circ-PLXNA1/miR-214/CTNNB1 in duck adipocyte but had little influence on triglyceride content and the adipogenic capacity of preadipocytes.…”

Section: Discussionmentioning

confidence: 99%

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

et al. 2020

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Adipocytes are derived from pluripotent mesenchymal stem cells through adipogenesis. Pre-adipocyte differentiation in poultry greatly influences fat deposition and meat quality. Circular RNAs (circRNAs) have an important function in cancer and some differentiation processes. Herein, high-throughput transcriptome sequencing was used to detect circRNAs present in cherry valley duck pre-adipocyte and adipocyte differentiation over 3 days. We identified 9,311 circRNAs and 141 differentially expressed circRNAs. Sequencing results were verified through qRT-PCR using seven randomly selected circRNAs, and competing endogenous RNA (ceRNA) networks were exhibited by ten important circRNAs in duck adipocyte differentiation. circRNA plexin A1 (circ-PLXNA1) was detected in duck adipocytes and mainly expressed in adipose, leg muscle and liver. Inhibition of circ-PLXNA1 limited the differentiation of duck adipocyte. There were four corresponding miRNAs for circ-PLXNA1 and 313 target genes for those miRNAs. CeRNA“circ-PLXNA1/miR-214/CTNNB1 axis” was focused and verified by a dual-luciferase reporter experiment. After co-transfection of cells with si-circ-PLXNA1 and miR-214 mimics, the expression level of CTNNB1 was down-regulated, triglyceride content and the adipogenic capacity of preadipocytes decreased. While there were no significant change after si-CTNNB1 transfection. All these results provide further insight into the circRNAs, especially for circ-PLXNA1 in duck adipocyte differentiation.

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Section: Discussionmentioning

confidence: 99%

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

et al. 2020

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“…In turn, the type and content of bovine adipose tissue directly affects meat tenderness and flavor [22]. miRNAs are important regulators of adipogenesis and lipid metabolism, with some of these miRNAs acting in a pro-adipogenic capacity (e. g., miR-214-3p, miR-20a-5p, and miR-144-3p) [23][24][25], whereas others are anti-adipogenic (e. g., miR-18b-3p, miR-22, and miR-27) [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

Shao

Fang

et al. 2020

Horm Metab Res

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MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs ~21 to ~23 nucleotides in length and have become a popular research topic in recent years due to their regulation of gene expression and many physiological processes, including fat metabolism; however, the precise functional mechanisms underlying their regulation of fat metabolism are not fully understood. Here, we identified miR-381, which specifically targets the 3′ untranslated region (3′ UTR) of potassium channel tetramerization-domain-containing 15 (KCTD15) , and verified the mechanism regulating its expression and participation in adipogenesis. We used a dual luciferase-reporter assay and transfection-mediated miR-381 overexpression and inhibition in Yanbian yellow cattle preadipocytes to investigate the role of miR-381 in adipogenesis. The results showed that miR-381 directly targets the 3′ UTR of KCTD15 and downregulates its expression. Additionally, miR-381 overexpression using an miRNA mimic promoted triglyceride accumulation and upregulated adipogenic peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT enhancer-binding protein α (C/EBPα) at both the protein and mRNA levels, whereas miR-381 inhibition produced the opposite effect. These results indicated that miR-381 regulates the differentiation of Yanbian yellow cattle preadipocytes by inhibiting KCTD15 expression, thereby highlighting the importance of miRNA-mediated regulation of adipogenesis. Furthermore, our findings suggested that miR-381 and its target gene(s) might represent new targets for investigating intramuscular fat deposits in cattle and treating human obesity.

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“…The miR-214-3p agomir, antagomir, and respective nonspecific control (NC) materials used in this study were purchased from GenePharma (GenePharma, Shanghai, China) and were transfected into GCs with X-treme GENE HP DNA Transfection Reagent (Roche, Mannheim, Germany) at a final concentration of 50 nmol according to the manufacturer's protocol. The medium was changed once after 24 h of transfection [21].…”

Section: Transfection Of Mirna Agomir and Antagomirmentioning

confidence: 99%

“…Research on miR-214-3p tends to center on oncology, skeletal muscle development, adipogenesis, and similar applications [19][20][21]. Sequencing results from the ovarian tissue of Yorkshire pigs have shown that the expression of miR-214-3p in ovary tissues of high-yielding sows is higher than that in low-yielding sows [22].…”

Section: Introductionmentioning

confidence: 99%

MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells

Shi

Zhou

et al. 2020

J Animal Sci Biotechnol

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Background Granulosa cells (GCs) proliferation and estradiol synthesis significantly affect follicular development. The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows, indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis. Results Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B (Cyclin B), cell cycle protein D (Cyclin D), cell cycle protein E (Cyclin E), and cyclin-dependent kinase 4 (CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1 (CYP11A1), cytochrome P450 family 19 subfamily A member 1 (CYP19A1), and steroidogenic acute regulatory protein (StAR) (i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively. Conclusions Our findings suggest that miR-214-3p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development.

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MicroRNA-214-3p Targeting Ctnnb1 Promotes 3T3-L1 Preadipocyte Differentiation by Interfering with the Wnt/β-Catenin Signaling Pathway

Cited by 38 publications

References 43 publications

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

Circular RNA expression profiling reveals that circ-PLXNA1 functions in duck adipocyte differentiation

miR-381 Targets KCTD15 to Regulate Bovine Preadipocyte Differentiation In Vitro

MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells

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