MicroRNA-214 Affects Fibroblast Differentiation of Adipose-Derived Mesenchymal Stem Cells by Targeting Mitofusin-2 during Pelvic Floor Dysfunction in SD Rats with Birth Trauma

Wu, Jing; Jiang, Li; Chen, Weikang; Liu, Shang; Liu, Jianhe; Zhang, Jingsong; Fang, Kewei

doi:10.1159/000479570

Cited by 20 publications

(16 citation statements)

References 34 publications

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“…It has been reported that mitofusin 2 (Mfn2), a gene related to mitochondrial fusion, is one of the target miR-214 in adiposederived mesenchymal stem cells. 18 We then measured the expression of Mfn2 in the kidney of various mice models, however, we found no difference among the groups. In mPTCs, overexpressing miR-214 had no effect on Mfn2 expression at both mRNA and protein levels (Supplementary Figure S6).…”

Section: Enhancing Mir-214 Induces Mitochondrial Dysfunction and Cellmentioning

confidence: 83%

MicroRNA-214 promotes chronic kidney disease by disrupting mitochondrial oxidative phosphorylation

Bai

Chen

Ding

et al. 2019

Kidney International

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Section: Enhancing Mir-214 Induces Mitochondrial Dysfunction and Cellmentioning

confidence: 83%

MicroRNA-214 promotes chronic kidney disease by disrupting mitochondrial oxidative phosphorylation

Bai

Chen

Ding

et al. 2019

Kidney International

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“…It has been argued that a single miRNA can simultaneously regulate the expression of multiple genes, and one gene can be regulated by multiple miRNAs [66]. miR-214 has been previously implicated in fibroblast differentiation of adipose-derived mesenchymal stem cells by targeting MFN2 [67], and regulates the differentiation of human hair follicle stem cells and bone marrow-derived mesenchymal stem cells [68,69].…”

Section: Discussionmentioning

confidence: 99%

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

Sun

Zhu

et al. 2020

Animals

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The excessive deposition of abdominal fat has become an important factor in restricting the production efficiency of chickens, so reducing abdominal fat deposition is important for improving growth rate. It has been proven that miRNAs play an important role in regulating many physiological processes of organisms. In this study, we constructed a model of adipogenesis by isolating preadipocytes (Ab-Pre) derived from abdominal adipose tissue and differentiated adipocytes (Ab-Ad) in vitro. Deep sequencing of miRNAs and mRNAs expressed in Ab-Pre and Ab-Ad groups was conducted to explore the effect of miRNAs and mRNAs on fat deposition. We identified 80 differentially expressed miRNAs (DEMs) candidates, 58 of which were up-regulated and 22 down-regulated. Furthermore, six miRNAs and six mRNAs were verified by qRT-PCR, and the results showed that the expression of the DEMs and differentially expressed genes (DEGs) in the two groups was consistent with our sequencing results. When target genes of miRNA were combined with mRNA transcriptome data, a total of 891 intersection genes were obtained, we predicted the signal pathways of cross genes enrichment to the MAPK signal pathway, insulin signal pathway, fatty acid metabolism, and ECM–receptor interaction. Meanwhile, we constructed miRNA and negatively correlated mRNA target networks, including 12 miRNA–mRNAs pairs, which showed a strong association with the abdominal adipocyte differentiation (miR-214−ACSBG2, NFKB2, CAMK2A, ACLY, CCND3, PLK3, ITGB2; miR-148a-5p−ROCK2; miR-10a-5p−ELOVL5; miR-146b-5p−LAMA4; miR-6615-5p−FLNB; miR-1774−COL6A1). Overall, these findings provide a background for further research on lipid metabolism. Thus, we can better understand the molecular genetic mechanism of chicken abdominal fat deposition.

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“…MicroRNA-214 is shown to increase expression of the mRNAs and proteins for elastin (tropoelastin), fibulin-5/DANCE, and LOX by targeting mitofusin-2 in the process of fibroblast differentiation of adipose-derived mesenchymal stem cells during pelvic floor dysfunction in Sprague Dawley rats with birth trauma [30]. In the present study, DCP-LA did not affect expression of the mRNAs and fibulin-5/DANCE.…”

Section: Discussionmentioning

confidence: 44%

PKCε Increases Extracellular Elastin and Fibulin-5/DANCE in Dermal Fibroblasts

Nishizaki¹

2018

Cell Physiol Biochem

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Background/Aims: In the earlier study, the selective PKCε activator DCP-LA increased elastic fibres in the dermis of HR-1 hairless mice. As a process of elastic fibre formation, tropoelastin, an elastin monomer, is secreted into the extracellular space. Secreted tropoelastin is delivered to the microfibrils by fibulin-5/developmental arteries and neural crest epidermal growth factor-like (DANCE) and undergoes self-association. Then, tropoelastin assembles around the microfibrils, growing into elastin and elastic fibres by lysyl oxidase (LOX)- or LOX-like (LOXL)-mediated cross-linking. The present study was conducted to understand the mechanism underlying DCP-LA-induced increase in elastin/elastic fibre. Methods: Western blotting, immunocytochemistory, and real-time reverse transcription-polymerase chain reaction (RT-PCR) were carried out in cultured human dermal fibroblasts. PKCε, mammalian target of rapamycin complex (mTOR), and p70 S6 kinase (S6K) were knocked-down by transfecting each siRNA. Results: DCP-LA increased elastin and fibulin-5/DANCE in a treatment time (6-24 h)- and a bell-shaped concentration (1 nM-1 µM)-dependent manner in the culture medium of human dermal fibroblasts. DCP-LA markedly increased elastic fibres in the extracellular space of cultured fibroblasts. DCP-LA-induced increase in extracellular elastin and fibulin-5/DANCE was abolished by a PKC inhibitor or knocking-down PKCε. DCP-LA did not affect expression of mRNAs for tropoelastin and fiblin-5/DANCE in cultured fibroblasts. DCP-LA-induced increase in extracellular elastin and fibulin-5/DANCE was not inhibited by the protein synthesis inhibitor cycloheximide or by knocking-down mTOR and S6K. DCP-LA never increased extracellular elastin in the presence of elastase, that breaks down elastin. An inhibitor of matrix metalloproteinase 9, that degrades multiple extracellular matrix components including elastin, had no effect on the basal levels and the DCP-LA-induced increase levels of extracellular elastin. Conclusion: The results of the present study indicate that PKCε, activated by DCP-LA, increases elastin and fibulin-5/DANCE in the extracellular space of cultured fibroblasts by the mechanism independent of transcriptional and translational modulation or inhibition of elastolysis.

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MicroRNA-214 Affects Fibroblast Differentiation of Adipose-Derived Mesenchymal Stem Cells by Targeting Mitofusin-2 during Pelvic Floor Dysfunction in SD Rats with Birth Trauma

Cited by 20 publications

References 34 publications

MicroRNA-214 promotes chronic kidney disease by disrupting mitochondrial oxidative phosphorylation

MicroRNA-214 promotes chronic kidney disease by disrupting mitochondrial oxidative phosphorylation

MiRNAs and mRNAs Analysis during Abdominal Preadipocyte Differentiation in Chickens

PKCε Increases Extracellular Elastin and Fibulin-5/DANCE in Dermal Fibroblasts

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