MicroRNA-218 inhibits the proliferation of human choriocarcinoma JEG-3 cell line by targeting Fbxw8

Shi, Dazun; Lü, Rong; Yang, Wenqing; Zhang, Yi

doi:10.1016/j.bbrc.2014.06.094

Cited by 16 publications

(12 citation statements)

References 39 publications

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“…An increasing volume of data has revealed the role of Fbxw8 E3 ligase in cancer (20,21,28,29). Impaired proliferation kinetics was identified in the Fbxw8 -/-mouse embryonic fibroblasts (30).…”

Section: Discussionmentioning

confidence: 99%

“…It was also revealed that miR-3160-5P inhibited DU145 cell proliferation by targeting Fbxw8. Target-inhibition of Fbxw8 arrested cell cycle progression at the G 2 /M phase in choriocarcinoma JEG3 cells by decreasing the expression level of G 2 /M-associated cell cycle regulators and increasing the expression level of p27 (20,21). The in-depth molecular mechanisms underlying cell cycle arrest induced by miR-3160-5P were investigated further.…”

Section: Discussionmentioning

confidence: 99%

“…Downregulation of Fbxw8 arrests cell cycle progression at the G 2 /M phase in JEG3 choriocarcinoma cells (20). miRNA-218, which targets Fbxw8, inhibits the proliferation of the human choriocarcinoma JEG-3 cell line (21).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8

et al. 2018

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Abstract. MicroRNAs (miRNAs/miRs), which are endogenous non-coding single-stranded RNAs 19-25 nucleotides in length, regulate gene expression by blocking translation or transcription repression. The present study revealed that miR-3160-5p was widely expressed in prostate cancer cells by reverse transcription-quantitative polymerase chain reaction. There was a negative association between the expression of miR-3160-5p and F-box and WD repeat domain containing 8 (Fbxw8) in prostate cancer DU145 cells. A luciferase activity assay was used to verify that Fbxw8 is the target of miR-3160-5p. In the present study, using MTT assay and cell cycle analysis, it was demonstrated that DU145 cell proliferation was repressed and the cell cycle was arrested in the G 2 /M cell cycle phase with upregulation of miR-3160-5p. Subsequent studies demonstrated that miR-3160-5p regulated the progression of the cell cycle in DU145 prostate cancer cells when the expression levels of phosphorylated cell division cycle (CDC)2, CDC25C and cyclin B1 were directly inhibited. Taken together, these findings revealed the mechanism underlying the role of miR-3160-5p in regulating the proliferation of DU145 cells and indicated that miR-3160-5p may serve as a promising novel therapeutic tool for prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8

et al. 2018

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“…In pancreatic cancer cells, FBXW8 increases the degradation of HPK1 (hematopoietic progenitor kinase 1) and promotes the cell growth (Wang et al, 2014a). Moreover, miR-218 targets the FBXW8 and inhibits the proliferation of human choriocarcinoma cells (Shi et al, 2014). …”

Section: Roles Of Fbxw Sub-family In the Regulation Of Cell Cyclementioning

confidence: 99%

Ubiquitination-mediated degradation of cell cycle-related proteins by F-box proteins

Zheng

Wang

Wei

2016

The International Journal of Biochemistry & Cell Biology

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F-box proteins, subunits of SKP1-cullin 1-F-box protein (SCF) type of E3 ubiquitin ligase complexes, have been validated to play a crucial role in governing various cellular processes such as cell cycle, cell proliferation, apoptosis, migration, invasion and metastasis. Recently, a wealth of evidence has emerged that F-box proteins is critically involved in tumorigenesis in part through governing the ubiquitination and subsequent degradation of cell cycle proteins, and dysregulation of this process leads to aberrant cell cycle progression and ultimately, tumorigenesis. Therefore, in this review, we describe the critical role of F-box proteins in the timely regulation of cell cycle. Moreover, we discuss how F-box proteins involve in tumorigenesis via targeting cell cycle-related proteins using biochemistry studies, engineered mouse models, and pathological gene alternations. We conclude that inhibitors of F-box proteins could have promising therapeutic potentials in part through controlling of aberrant cell cycle progression for cancer therapies.

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“…MiR-218 downregulates FBXW8 in human JEG-3 choriocarcinoma cells and promotes cell proliferation [87]. Since FBXW8 mediates the ubiquitination and degradation of p27 [88], the miR-218 mediated decrease of FBXW8 would be expected to lead to increased p27 levels, as well as impaired cell cycle arrest and enhanced JEG-3 cell proliferation.…”

Section: Mirna-dependent Regulation Of F-box Proteins and Its Rolementioning

confidence: 99%

MicroRNA regulation of F-box proteins and its role in cancer

Pfeffer

2016

Seminars in Cancer Biology

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MicroRNAs (miRNAs) are small endogenous non-coding RNAs, which play critical roles in cancer development by suppressing gene expression at the post-transcriptional level. In general, oncogenic miRNAs are upregulated in cancer, while miRNAs that act as tumor suppressors are downregulated, leading to decreased expression of tumor suppressors and upregulated oncogene expression, respectively. F-box proteins function as the substrate-recognition components of the SKP1-CUL1-F-box (SCF)-ubiquitin ligase complex for the degradation of their protein targets by the ubiquitin-proteasome system. Therefore F-box proteins and miRNAs both negatively regulate target gene expression post-transcriptionally. Since each miRNA is capable of fine-tuning the expression of multiple target genes, multiple F-box proteins may be suppressed by the same miRNA. Meanwhile, one F-box proteins could be regulated by several miRNAs in different cancer types. In this review, we will focus on miRNA-mediated downregulation of various F-box proteins, the resulting stabilization of F-box protein substrates and the impact of these processes on human malignancies. We provide insight into how the miRNA: F-box protein axis may regulate cancer progression and metastasis. We also consider the broader role of F-box proteins in the regulation of pathways that are independent of the ubiquitin ligase complex and how that impacts on oncogenesis. The area of miRNAs and the F-box proteins that they regulate in cancer is an emerging field and will inform new strategies in cancer treatment.

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MicroRNA-218 inhibits the proliferation of human choriocarcinoma JEG-3 cell line by targeting Fbxw8

Cited by 16 publications

References 39 publications

Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8

Prostate cancer cell proliferation is suppressed by microRNA‑3160‑5p via targeting of F‑box and WD repeat domain containing 8

Ubiquitination-mediated degradation of cell cycle-related proteins by F-box proteins

MicroRNA regulation of F-box proteins and its role in cancer

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