Microrna-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl cpg binding protein 2

Zhao, Hanqing; Huang, Yuan; Yu, Xinyan; Wen, G.; Ye, S.; Zhang, L.; Xiao, Qingzhong

doi:10.1016/j.atherosclerosis.2014.05.364

Cited by 1 publication

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References 225 publications

(330 reference statements)

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“…However, in smooth muscle, miR-22 promotes contractile differentiation by down-regulating methyl CpG-binding protein 2 (MECP2) [71] suggesting that miR-22 may play a role in both hypertrophic growth and contractile differentiation. Previous studies from our group have demonstrated that actin/MRTF signaling is involved in simultaneous contractile differentiation and hypertrophic growth following mechanical stretch of the vascular wall [15,16,72].…”

Section: Several Of the Actin/mrtf-a-sensitive Mirnas Identified In Tmentioning

confidence: 99%

Regulation of microRNA expression in vascular smooth muscle by MRTF-A and actin polymerization

Alajbegovic

Turczyńska

Hien

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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The dynamic properties of the actin cytoskeleton in smooth muscle cells play an important role in a number of cardiovascular disease states. The state of actin does not only mediate mechanical stability and contractile function but can also regulate gene expression via myocardin related transcription factors (MRTFs). These transcriptional co-activators regulate genes encoding contractile and cytoskeletal proteins in smooth muscle. Regulation of small noncoding microRNAs (miRNAs) by actin polymerization may mediate some of these effects. MiRNAs are short non-coding RNAs that modulate gene expression by post-transcriptional regulation of target messenger RNA.In this study we aimed to determine a profile of miRNAs that were 1) regulated by actin/MRTF-A, 2) associated with the contractile smooth muscle phenotype and 3) enriched in muscle cells. This analysis was performed using cardiovascular disease-focused miRNA arrays in both mouse and human cells. The potential clinical importance of actin polymerization in aortic aneurysm was evaluated using biopsies from mildly dilated human thoracic aorta in patients with stenotic tricuspid or bicuspid aortic valve.By integrating information from multiple qPCR based miRNA arrays we identified a group of five miRNAs (miR-1, miR-22, miR-143, miR-145 and miR-378a) that were sensitive to actin polymerization and MRTF-A overexpression in both mouse and human smooth muscle. With the exception of miR-22, these miRNAs were also relatively enriched in striated and/or smooth muscle containing tissues. Actin polymerization was found to be dramatically reduced in the aorta from patients with mild aortic dilations. This was associated with a decrease in actin/MRTF-regulated miRNAs.In conclusion, the transcriptional co-activator MRTF-A and actin polymerization regulated a subset of miRNAs in smooth muscle. Identification of novel miRNAs regulated by actin/MRTF-A may provide further insight into the mechanisms underlying vascular disease states, such as aortic aneurysm, as well as novel ideas regarding therapeutic strategies.

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Section: Several Of the Actin/mrtf-a-sensitive Mirnas Identified In Tmentioning

confidence: 99%

Regulation of microRNA expression in vascular smooth muscle by MRTF-A and actin polymerization

Alajbegovic

Turczyńska

Hien

et al. 2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

View full text Add to dashboard Cite

show abstract

Microrna-22 regulates smooth muscle cell differentiation from stem cells by targeting methyl cpg binding protein 2

Cited by 1 publication

References 225 publications

Regulation of microRNA expression in vascular smooth muscle by MRTF-A and actin polymerization

Regulation of microRNA expression in vascular smooth muscle by MRTF-A and actin polymerization

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