MicroRNA-221 and -222 Regulate Radiation Sensitivity by Targeting the PTEN Pathway

MicroRNAs and Their Impact on Radiotherapy for Cancer

“…For instance, PTEN has been proven to be a target of miR-221/-222. Knockout of these miRNAs results in increased PTEN levels, which in turn lead to suppressed Akt activity and increased apoptosis, and most importantly, to enhanced radiosensitivity in various cancer cell lines (57). These results are in agreement with observations by other authors reporting studies using gastric (58) and colorectal cancer cell lines (54).…”

Unveiling the non-repair face of the Base Excision Repair pathway in RNA processing: A missing link between DNA repair and gene expression?

“…We found more than 1000 APE1-bound RNA among which many ncRNAs and notably pri-miRNAs that are directly bound by APE1 in cancer cells. In particular, we showed that APE1 endonuclease activity over pri-miR-221/222 mediates the regulation of the tumor suppressor PTEN, a known target of these miRNAs [97]. Moreover, we evidenced, for the first time, that APE1 associates with the Drosha microprocessor complex during oxidative stress suggesting a possible contribution of APE1 in RNA-decay pathways controlling miRNAs precursors stability in the genotoxic cell response.…”

Ionizing Radiations Induce Apoptosis in TRAIL Resistant Cancer Cells: in vivo and in vitro Analysis

“…miRNA studies have started to expand our understanding about the fact that PTEN is negatively regulated by miR-221/miR-222. Therefore targeted inhibition of miR-221/ miR-222 is effective in enhancing radio-sensitivity (Zhang et al, 2011).…”