MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

Løvendorf, Marianne B.; Zibert, John R.; Gyldenløve, Mette; Røpke, Mads A.; Skov, Lone

doi:10.1016/j.jdermsci.2014.05.005

Cited by 90 publications

(95 citation statements)

References 49 publications

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“…Although not in psoriasis, miR‐21 was positively regulated by Stat3, and SHIP2 was targeted by miR‐205 in apoptosis . miR‐143 was predicted to target SLC26A4 and miR‐223 with GLUL, SMAD3 . Based on the miRNA–mRNA or miRNA–protein target interactions (as indicated in Table and Table with *), miRNAs are potential regulators in the cell signalling pathways.…”

Section: Immunological Function Of Mirna In Psoriasismentioning

confidence: 99%

“…miR‐223 and miR‐143 were found to significantly upregulate in the PBMCs from patients with psoriasis, and these miRNA significantly correlated with PASI score. ROC analysis showed that miR‐223 and miR‐143 have the potential to distinguish between psoriasis and healthy controls . Hence, it is suggested that miR‐223 and miR‐143 may serve as a novel biomarker for disease activity of psoriasis .…”

Section: Mirna As Potential Biomarkers In Psoriasismentioning

confidence: 99%

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Roles of microRNAs in psoriasis: Immunological functions and potential biomarkers

Liu

Han

et al. 2017

Experimental Dermatology

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MicroRNAs (miRNAs) are small non-coding RNA molecules, which function in RNA silencing and post-transcriptional regulation of gene expression. Psoriasis is an inflammatory skin disease characterized by the dysfunction of keratinocytes, with the immune dysregulation. We reviewed the recent studies on the roles of miRNAs in psoriasis and showed that miRNAs play key roles in psoriasis, including the regulation of hyperproliferation, cytokine and chemokine production in keratinocyte, as well as mediating immune dysfunction in psoriasis. Furthermore, miRNAs, particularly, circulating miRNAs may serve as novel biomarkers for diagnosis, monitoring therapy response and reflecting the disease severity. Thus, targeting specific miRNAs may be used to develop new therapeutic methods for psoriasis.

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Section: Immunological Function Of Mirna In Psoriasismentioning

confidence: 99%

Section: Mirna As Potential Biomarkers In Psoriasismentioning

confidence: 99%

Roles of microRNAs in psoriasis: Immunological functions and potential biomarkers

Liu

Han

et al. 2017

Experimental Dermatology

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“…4,5 However, to our knowledge, no study has been published yet, showing that specific secretory miRs become deregulated in the course of skin ageing and are involved in human skin cell crosstalk. Thus, we analysed, if secreted miRs might play a role in age-related dysfunctions.…”

Section: Questions Addressedmentioning

confidence: 99%

Identification of miR‐126 as a new regulator of skin ageing

Fiedler

Grönniger

Pfanne

et al. 2017

Experimental Dermatology

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Movie S2 This is similar to movie 1, but with a more direct interaction. This is to indicate the heterogeneous nature of the nerve interactions with keratinocytes Movie S3 A 3-D resolution of nerve fiber innervation of keratinocytes showing synaptic bouton-like structure penetrating and exiting keratinocytes; corresponds to figure 2dMovie S4 A bottom to top Z projection of the sample from figure 2d and Movie 3, indicating that the nerve fiber could be within one or | QUESTIONS ADDRESSEDA deregulation of miR expression in skin-associated diseases such as psoriasis and atopic dermatitis has been reported previously. 4,5However, to our knowledge, no study has been published yet, showing that specific secretory miRs become deregulated in the course of skin ageing and are involved in human skin cell crosstalk. Thus, we analysed, if secreted miRs might play a role in age-related dysfunctions. | EXPERIMENTAL DESIGNWe generated miR profiles of interstitial fluid collected from younger and older volunteers after suction blistering and identified agedependent differences in miR levels (eg miR-126). In vitro, we analysed whether miR-126 is age-dependently secreted by dermal endothelial cells and incorporated by other skin cells. | RESULTS | Secretory miR levels during skin ageingTo investigate the levels of secreted miRs during skin ageing, human interstitial fluid of the skin was analysed. 6 Quantitative PCR-based microRNA profiling was performed, and 175 miRs were detected with a C t -value <40 which was sufficient to be considered in the following analysis ( Figure S1 and Table S1). Setting a threshold of 1.5-fold regulation (up/down), we identified 61 miRs that were regulated in an age-related manner in suction blister fluids collected from young and old volunteers. The results could be confirmed in an independent experiment for three selected miRs (miR-100, miR-126 and miR-223) | Age-related miR-100 , miR-126 and miR-223 levels in cultured endothelial cellsIn vitro analysis was performed to delineate whether the age-related miR expression changes are triggered during cellular ageing. We cultivated dermal endothelial cells isolated from volunteers aged between 22 and 73 years and determined miR-100, miR-126 and miR-223 abundance in the supernatant 48 hours after cell seeding. All three miRs were significantly decreased in the supernatant of endothelial cell populations isolated from aged volunteers compared to the younger cell population ( Figure S2A-C). In contrast, intra-cellular miR levels detected for miR-100 and miR-126 (miR-223 was not analysed) in the intra-cellular compartment remain stable throughout age ( Figure S3A-B).*contributed equally to this work F I G U R E 1 Age-related miR expression changes in human suction blister fluids of the skin. A-C: Different miRs were selected from miRNA profiling for validation from qRT-PCR profiling. The suction blister fluid of 20 healthy volunteers of two different age groups (18-28 years and 66-75 years) was collected, and the suction blister fluids of 2 volunteers ...

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“…Moreover, Cesare et al revealed that STAT3 was associated with psoriasis through the IL-23 signaling pathway [21]. Recently, the relationship between miRNA and psoriasis has stimulated great interest [22, 23], However, according to TargetScan (http:// www.targetscan.org/vert_71/), few studies have investigated the target relationship between miRNAs and STAT3, and we still do not know which specific miRNAs target STAT3 in psoriasis.…”

Section: Introductionmentioning

confidence: 99%

Let-7a Inhibits T-Cell Proliferation and IFN-γ Secretion by Down-Regulating STAT3 Expression in Patients with Psoriasis

Xie

Chen

et al. 2017

Cell Physiol Biochem

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Objective: This study aimed to explore the effects of STAT3 targeting by let-7a on T-cell proliferation and IFN-γ secretion in psoriasis. Methods: From January 2013 to January 2015, 40 patients with psoriasis (psoriasis group) and 38 volunteers undergoing plastic surgery (control group) were enrolled in this study. Pearson correlation analysis was performed to evaluate the correlation between let-7a and STAT3 expression. T-cells were isolated and subjected to different transfection methods. A dual luciferase reporter assay was carried out to confirm STAT3 as a target gene of let-7a. Let-7a, STAT3 and IFN-γ mRNA expression was detected by quantitative real-time fluorescent polymerase chain reaction (qRT-PCR), and pSTAT3 protein levels were determined by Western blot. T-cell proliferation was evaluated with a cell counting kit-8 (CCK-8) assay. Results: The level of STAT3 mRNA and pSTAT3 was higher, but let-7a expression was lower in the psoriasis group than the control group. Pearson correlation analysis indicated that STAT3 expression was negatively correlated with let-7a expression. T-cells transfected with inhibitors exhibited greater IFN-γ mRNA expression and T-cell proliferation than transfected T-cells and T-cells transfected with a non-sense sequence, while T-cells transfected with let-7a mimics exhibited lower IFN-γ mRNA expression and T-cell proliferation than transfected T-cells and T-cells transfected with a non-sense sequence. This suggested that siRNA-STAT3 could reverse the increase in IFN-y mRNA expression and T-cell proliferation induced by let-7a inhibitors. Conclusion: Our results demonstrated that let-7a inhibits T-cell proliferation and IFN-γ secretion by down-regulating STAT3 in psoriasis.

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MicroRNA-223 and miR-143 are important systemic biomarkers for disease activity in psoriasis

Cited by 90 publications

References 49 publications

Roles of microRNAs in psoriasis: Immunological functions and potential biomarkers

Roles of microRNAs in psoriasis: Immunological functions and potential biomarkers

Identification of miR‐126 as a new regulator of skin ageing

Let-7a Inhibits T-Cell Proliferation and IFN-γ Secretion by Down-Regulating STAT3 Expression in Patients with Psoriasis

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