MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

Cai, Tonghui; Guan, Xiaoyingï¿1⁄2; Wang, Hongyanï¿1⁄2; Fang, Yingï¿1⁄2; Long, Jieï¿1⁄2; Xie, Xiaobinï¿1⁄2; Zhang, Yajieï¿1⁄2

doi:10.3892/ol.2018.8048

Cited by 3 publications

(4 citation statements)

References 43 publications

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“…Data from several studies previously showed that miR-223 does not only promote the invasion of lung cancer cells but also the metastasis of gastric cancer via targeting tumor suppressor DAL-1 (8,9). Our previous study demonstrated that both miR-26a and DAL-1 gene expression are decreased in NSCLC, and DAL-1 is not a real target gene of miR-26a (10). Both miR-26b and miR-26a belong to the miR-26 family, and miR-26b has low expression levels in many types of cancer, such as epithelial ovarian (EOC) (11), hepatocellular carcinoma (HCC) (12), as well as colorectal cancer (13).…”

Section: Introductionmentioning

confidence: 94%

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

et al. 2017

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Lung cancer is the leading cause of cancer-related death worldwide. The poor prognosis is partly due to lack of efficient methods for early diagnosis. MicroRNAs play roles in almost all aspects of cancer biology, and can be secreted into the circulation and serve as molecular biomarkers for the early diagnosis of cancer. In the present study, we determined the expression of miR-96 and the function of its target genes in lung cancer through bioinformatic analysis. Four microRNA expression profiles of lung cancer were downloaded from Gene Expression Omnibus and the data were analyzed using SPSS 16.0 software. Compared to the control group, expression of miR-96 was significantly increased in non-small cell lung cancer (NSCLC) (GSE51855), lung adenocarcinoma (GSE48414), stage I adenocarcinoma tissues (GSE63805) and the plasma of lung cancer patients (GSE68951). miR-96 was also elevated in six different NSCLC cell lines. However, the expression level of miR-96 was not related to the age, gender, clinical stage and histological subtype of the NSCLC patients. GO analysis of 78 predicted target genes of miR-96 showed that 42 of the obtained GO terms are highly associated with specific cellular processes including response to stimulus, signaling pathway, cell division, cell communication, cell migration and calcium signaling. KEGG results indicated that the miR-96 targets are mainly involved in the GnRH signaling pathway, long-term potentiation and insulin signaling pathway. In conclusion, miR-96, functioning as an oncogene, may play an important role in the development and progression of lung cancer. miR-96 may have the potential to serve as a molecular biomarker for the early diagnosis of NSCLC.

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Section: Introductionmentioning

confidence: 94%

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

et al. 2017

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“…27 ANXA1 was dysregulated in various diseases, highlighting its significant function in tumor progression. 28 ANXA1 expression could be quite different in cancers, and the opposite expression of ANXA1 is the only one of the mysteries of B, Western blot analysis of related protein expression in each group; *P < 0.05 vs the blank group; measurement data were expressed as mean ± standard deviation, data of multiple group data were compared using one-way ANOVA, and the experiment was repeated three times. ANOVA, analysis of variance; miR, microRNA its potential regulatory mechanisms and phenotypic specificity.…”

Section: Discussionmentioning

confidence: 99%

microRNA‐206 overexpression inhibits epithelial‐mesenchymal transition and glomerulosclerosis in rats with chronic kidney disease by inhibiting JAK/STAT signaling pathway

Zhao

Shen

Gao

et al. 2019

J of Cellular Biochemistry

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Chronic kidney disease (CKD) is a traumatic disease with significant psychic consequences to the patient's overall physical condition. microRNA‐206 (miR‐206) has been reported to play an essential role in the development of various diseases. The purpose of the present study is to investigate the effect of miR‐206 through the JAK/STAT signaling pathway on epithelial‐mesenchymal transition (EMT) of renal tubular epithelial cells and glomerulosclerosis in rats with CKD. The targeting relationship between miR‐206 and ANXA1 was verified. To explore the role of miR‐206 in CKD, the model of CKD rats was established to detect glomerular sclerosis index (GSI), contents of interleukin‐6 (IL‐6) and transforming growth factor‐beta1 (TGF‐β1), and expression of type IV collagen. Moreover, to further determine the roles of both miR‐206 and the JAK/STAT signaling pathway in CKD, the gain‐ and loss‐of function approaches were performed with the expression of ANXA1, α‐SMA, E‐cadherin, vimentin, N‐cadherin, and the JAK/STAT signaling pathway‐related genes detected. miR‐206 negatively targeted ANXA1. Overexpressed miR‐206 inhibited the degeneration and interstitial fibrosis of renal tubular epithelial cells, decreased GSI of rats, and the expression of type IV collagen, TGF‐β1 and IL‐6. Overexpressed miR‐206 inhibited the degeneration of renal tubular epithelial cells, the expression of ANXA1, α‐SMA, TGF‐β1, p‐STAT3, STAT3, p‐STAT1, STAT1, p‐JAK2, and JAK2, while promoted the expression of E‐cadherin. Taken together the results, miR‐206 inhibits EMT of renal tubular epithelial cells and glomerulosclerosis by inactivating the JAK/STAT signaling pathway via ANXA1 in CKD.

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“…Expression levels of ANXA1 detected in different cancers are not consistent. In certain tumors such as head and neck, esophageal squamous cell and prostate cancers they are down regulated and in others like glioma and oropharngeal cancers they are up regulated 6…”

Section: Introductionmentioning

confidence: 99%

“…In certain tumors such as head and neck, esophageal squamous cell and prostate cancers they are down regulated and in others like glioma and oropharngeal cancers they are up regulated. 6 Pakistan is a multiethnic state with five provinces and different ethnicities. This study was aimed to assess the risk association of miR-196a rs11614913 C/T variant and the relationship of miR-196a genotype with expression of its target gene ANXA1 in BC cases.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients

2019

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Objective: To evaluate the association of miR-196a rs11614913 C/T genetic variation and its target gene annexin A1 mRNA expression with breast cancer risk in Pakistani female ethnicities. Methods: This case control study, conducted from March 2017 to November 2018 included 295 breast cancer patients, 295 controls of three Pakistani ethnicities and archived 100 samples of cohort group for genotyping and expression profiling. Genotyping of miR-196a (rs11614913 C/T) was done by ARMS PCR technique. Annexin-A1 (ANXA1) mRNA expression was measured with qRT-PCR and detection of protein expression of ANXA1 was done by immunohistochemistry. Results: CC homozygous genotype of miR-196a rs11614913 was present in 81.4% of cases and 73.9% controls. C/T polymorphism was found to be significantly associated with decrease risk of breast cancer (OR=0.25 (0.11- 0.58, p <0.05). Similar trend was seen with the minor T allele (OR=0.55 (0.39-0.77, p <0.05, and both dominant and recessive models (OR=0.64; p=0.02 and OR=0.26, p=0.00). In the KPK ethnic group significant decrease association with breast cancer risk was observed (OR= 0.22 (0.09-0.53, p < 0.05). Immunohistochemical staining showed loss of ANXA1 protein expression in 72 samples, and significant association was observed with pathological type p=0. 00 and triple negative receptor status p=0.03 and with genotypes of miR-196a p=0.00. Increase relative expression of 2.81± .88 by qPCR analysis of ANXA1 mRNA was noted with TT genotype. Conclusions: Our results demonstrate that miR-196a rs11614913 C/T polymorphism is associated with a decreased risk and loss of protein expression in breast cancer in the Pakistani population. doi: https://doi.org/10.12669/pjms.35.6.1322 How to cite this:Rahim A, Afzal M, Naveed AK. Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients. Pak J Med Sci. 2019;35(6):1598-1604. doi: https://doi.org/10.12669/pjms.35.6.1322 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MicroRNA‑26a regulates ANXA1, rather than DAL‑1, in the development of lung cancer

Cited by 3 publications

References 43 publications

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

Identification and characterization of miR-96, a potential biomarker of NSCLC, through bioinformatic analysis

microRNA‐206 overexpression inhibits epithelial‐mesenchymal transition and glomerulosclerosis in rats with chronic kidney disease by inhibiting JAK/STAT signaling pathway

Genetic polymorphism of miRNA-196a and its target gene annexin-A1 expression based on ethnicity in Pakistani female breast cancer patients

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