MicroRNA-27a participates in the pathological process of depression in rats by regulating VEGFA

Cui, Jian; Gong, Cunqi; Cao, Baorui; Li, Longfei

doi:10.3892/etm.2018.5942

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…16 fed dams. We report that a reduction in miRNA27a in response to Exercise and PregEx matched a reciprocal increase in VEGFA protein expression, an association that was recently validated in rat hippocampal tissue [6]. The role of miRNAs in regulating placental vasculogenesis and angiogenesis are yet to be fully explored.…”

Section: Accepted Manuscriptmentioning

confidence: 67%

“…Studies in Plgf -/mice demonstrate the importance of PlGF during placental blood vessel development, where these mice have reduced implantation sites and placental vascular branching as well as increased blood spaces [5]. Recent studies demonstrate that VEGFA expression can be modulated by micro RNAs (miRNA), including miRNA27a [6], which may implicate this miRNA in altered vasculogenesis and/or angiogenesis in complicated pregnancies.…”

Section: Introductionmentioning

confidence: 99%

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Maternal exercise and growth restriction in rats alters placental angiogenic factors and blood space area in a sex-specific manner

et al. 2018

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Section: Accepted Manuscriptmentioning

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Maternal exercise and growth restriction in rats alters placental angiogenic factors and blood space area in a sex-specific manner

et al. 2018

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“…Postmortem brain studies revealed that the miRNA-27a expression was downregulated in the prefrontal cortex of depressed suicide subject [ 41 ]. Depressed rats exhibited decreased levels of miRNA-27a in the hippocampus and peripheral blood [ 42 ]. Honokiol, a bioactive polyphenolic substance, significantly shortened the immobility duration of LPS mice in both TST and FST experiments, which was strongly related to decreased NF-κB activity in the hippocampus [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a/SYK/NF-κB axis

Song

Tong

et al. 2021

J Neuroinflammation

180

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Background The NLRP3-mediated pyroptosis, which could be regulated by miRNA-27a, is a key player in the development of depression. Isoliquiritin is a phenolic flavonoid compound that has been demonstrated to suppress NLRP3-mediated pyroptosis. However, it is still unknown whether isoliquiritin could confer antidepressant activity via decreasing NLRP3-mediated pyroptosis by stimulating miRNA-27a. Thus, in the current study, we explored the antidepressant activity of isoliquiritin and its underlying mechanism. Methods Expression of miRNA-27a in depressed patients or mice was measured using qRT-PCR. Luciferase reporter assay was performed to illustrate the link between miRNA-27a and SYK. Lipopolysaccharide (LPS) and chronic social defeat stress (CSDS) depression models were established to investigate the antidepressant actions of isoliquiritin. Changes in miRNA-27a/SYK/NF-κB axis and NLRP3-mediated pyroptosis were also examined. The role of miRNA-27a in isoliquiritin-related antidepressant effect was further investigated by using miRNA-27a inhibitors and mimics of miRNA-27a. Results Our results showed the miRNA-27a expression was downregulated in the serum of depressed patients, and decreased serum and hippocampus expression of miRNA-27a were observed in rodent models of depression. SYK gene expression was significantly reduced by miRNA-27a mimic incubation. Isoliquiritin profoundly attenuated LPS or CSDS-induced depressive symptoms, as well as CSDS-induced anxiety behavior. In the hippocampus, LPS and CSDS decreased miRNA-27a mRNA expression; increased the protein levels of SYK, p-NF-κB, and NLRP3: cleaved Caspase-1, IL-1β, and GSDMD-N: and elevated the concentration of IL-1β, IL-6, and TNF-α, which were all restored by isoliquiritin administration. Meanwhile, isoliquiritin upregulated the hippocampal NeuN protein level, improved the survival and morphology of neurons, and decreased pyroptosis-related neuronal cell death. Moreover, isoliquiritin protected primary microglia against LPS and adenosine triphosphate (ATP) elicited NLRP3 inflammasome activation in vitro, evidenced by declined protein levels of p-NF-κB, NLRP3; cleaved Caspase-1, IL-1β, and GSDMD-N; upregulated miRNA-27a mRNA expression; and decreased the mRNA and protein levels of SYK. Nevertheless, miRNA-27a inhibitors significantly reversed isoliquiritin-generated therapeutic efficacy in CSDS mice and in vitro. Furthermore, the cytoprotective effect of isoliquiritin was similar to that of miRNA-27a mimics in LPS and ATP-treated primary microglia. Taken together, these findings suggest that isoliquiritin possesses potent antidepressant property, which requires miRNA-27a/SYK/NF-κB axis controlled decrease of pyroptosis via NLRP3 cascade.

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“…MicroRNA is small nucleic acids consisting of about ~ 20 nucleic acids, which involved in various biological pathways, and recent studies have reported that miRNA plays a key role in neurological diseases. In this study, we found that the expressions of miRNAs were reversed its abnormal changes by DON and SIP3L cotreatment, including miRNA-15a, -27a, 32, which are linked with in ammation or apoptosis pathways [46][47][48][49], indicating that the therapeutic mechanism of SIP3 and DON might underlie miRNA regulation. Further studies are required to unravel the detailed mechanism.…”

Section: Discussionmentioning

confidence: 67%

Co-treatment With the Herbal Medicine SIP3 and Donepezil Improves Memory and Depression in the Mouse Model of Alzheimer’s Disease

Liu

Choi

Son

et al. 2021

Preprint

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Background: Alzheimer’s disease (AD) is a lethal progressive neurodegenerative disorder. Currently, many acetylcholinesterase inhibitors, such as donepezil, is widely used for the treatment of AD. However, the efficacy of long-term donepezil use is limited. SIP3, a mixture of Santalum album, Illicium verum, and Polygala tenuifolia, a new formula derived from traditional Korean herbal medicine. In this study, SIP3 were assessed the survival of Drosophila AD model and synergistic effect of SIP3, donepezil co-treatment of AD using APP/PS1 transgenic mice. Methods: In Drosophila AD models, we analyzed the survival, climbing ability and acridine orange (AO) staining. In APP/PS1 mice, at six months of age were randomized into four groups. Then, these groups were orally administered vehicle (for the control), donepezil, low and high doses SIP3 plus Donepezil respectively for six months. The passive avoidance test (PAT) and the Morris water maze (MWM) were analyzed cognitive behavioral changes. In addition, the forced swimming test (FST) and the tail suspension test (TST) were assessed depression-like behavior. To investigate the molecular and cellular mechanisms underlying positive effects of SIP3 on AD, the cerebral cortex transcriptomes were analyzed by RNA sequencing.Results: Using the passive avoidance test (PAT), we analyzed the combination of SIP3 and donepezil improved the learning capabilities and memory of APP/PS1 mice, compared with the group treated with donepezil only, in late stage of AD. In addition, using the Morris water maze (MWM) test, co-treatment with donepezil and a low concentration of SIP3 significantly ameliorated cognitive impairment. Co-administration of SIP3 and donepezil effectively reduced depression-like behavior in the forced swimming and tail suspension tests. Furthermore, RNA sequencing of cerebral cortex transcriptome revealed that gene expression profiles after low dose of SIP3 co-treatment are slightly similar to those of normal phenotype mice than those obtained after donepezil treatment alone. Gene ontology (GO) along with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway have demonstrated that differentially expressed genes were involved in locomotor behavior and neuroactive ligand-receptor interactions. Collectively: our results suggest that co-treatment of low dose of SIP3 and donepezil improves impaired learning, memory, and depression in late stage of AD in mice.

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MicroRNA-27a participates in the pathological process of depression in rats by regulating VEGFA

Cited by 9 publications

References 49 publications

Maternal exercise and growth restriction in rats alters placental angiogenic factors and blood space area in a sex-specific manner

Maternal exercise and growth restriction in rats alters placental angiogenic factors and blood space area in a sex-specific manner

Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a/SYK/NF-κB axis

Co-treatment With the Herbal Medicine SIP3 and Donepezil Improves Memory and Depression in the Mouse Model of Alzheimer’s Disease

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