MicroRNA‑27b‑3p inhibits the proliferation and invasion of cutaneous squamous cell carcinoma by targeting EGFR and MMP‑13

Abstract

Cutaneous squamous cell carcinoma is a common malignant tumor. The aim of the present study was to examine the biological function of microRNA (miR)-27b-3p in cutaneous squamous cell carcinoma (CSCC) and its underlying mechanism. The relative expression levels of miR-27b-3p were determined in A-431, Colo-16 and NHEK/SVTERT3-5 cell lines. The regulatory effects of miR-27b-3p on the proliferation of CSCC cells were evaluated using MTT and colony formation assays. Transwell assays were conducted to examine the role of miR-27b-5p in the migratory and invasive abilities of CSCC cells. The levels of EGFR, MMP-13, Akt, phosphorylated (p)-Akt, cyclin D1, N-cadherin (CAD) and E-CAD were detected in CSCC cells using reverse transcription-quantitative PCR and western blot analysis. Binding between miR-27b-3p and the 3'-untranslated region (UTR) of EGFR or MMP-13 was assessed using a dual-luciferase reporter assay. miR-27b-3p was significantly downregulated in CSCC cell lines, compared with the skin keratinocyte cell line. Transfection with a miR-27b-3p mimic significantly reduced the proliferative, migratory and invasive abilities of CSCC cells in vitro. Moreover, miR-27b-3p mimic transfection downregulated the mRNA and protein levels of EGFR, MMP-13, cyclin D1, p-Akt and N-CAD, whilst upregulating E-CAD levels in CSCC cells. miR-27b-3p was found to target the EGFR and MMP-13 3'-UTRs, thus downregulating the expression of these molecules. The inhibition of CSCC proliferation by miR-27b-3p was effectively reversed by EGFR overexpression. Moreover, the inhibitory effect of miR-27b-3p on the migratory and invasive abilities of CSCC cells was abolished by MMP-13 overexpression. In conclusion, miR-27b-3p inhibits the proliferation, migration and invasion of CSCC cells by downregulating the expression of EGFR and MMP-13 and may represent a potential diagnostic marker and therapeutic option for CSCC.

Molecular mechanisms of miR‐1236 in the assessment of tumor lymphangiogenesis in human ovarian cancer patients

“…Consistent with our results, previous reports have indicated a significant association between the expression level of miR‐1236 and the survival of cancer patients 20,29,30 . For example, Liang et al 32 demonstrated that decreased miR‐1236 expression is associated with poor prognosis in breast cancer. Furthermore, the downregulation of miR‐1236 has been correlated with poorer survival of colon cancer patients, and significantly worse survival rates of gastric cancer patients with low expression of miR‐1236 compared with those with high expression have been reported 20,29,30 .…”

“…Downregulation of miR‐1236 was also found in renal cell carcinoma and correlated with tumor stage, tumor grade and LN metastasis 31 . Other malignancies in which decreased miR‐1236 expression was detected include hepatocellular carcinoma, lung cancer and breast cancer 17,28,32 . The decreased miR‐1236 expression and its association with unfavorable clinical–pathological features in OC patients observed in our current study suggest that miR‐1236 could serve as a potential marker for the diagnosis and prognosis of OC.…”

High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma

“…Through correlation analysis, expression level analysis and prognostic analysis, we found that hsa-miR-27b-3p was the most likely upstream tumor suppressor miRNA of C1QTNF1. At present, several studies have shown that miR-27b-3p plays the role of a tumor suppressor miRNA ( Sun et al, 2017 ; Liang and Zhang, 2021 ). Based on the ceRNA hypothesis, 143 lncRNAs upstream of hsa-miR-27b-3p/C1QTNF1 were predicted.…”