MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39

Li, Chenxing; Wang, Aiying; Chen, Yanke; Liu, Yan; Zhang, Hui; Zhou, Jun

doi:10.3892/or.2020.7486

Cited by 19 publications

(18 citation statements)

References 42 publications

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“…In this study, an online database was used to nd potential target miRNAs of SNHG19 and selected miR-299-5p. Previous studies have established the inhibitory function of miR-299-5p in human cancers, including head and neck squamous cell carcinoma [22], breast cancer [23], papillary thyroid cancer [24]. In SNHG19-knockdown breast cancer cells, the expression of miR-299-5p was signi cantly increased.…”

Section: Discussionmentioning

confidence: 97%

Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p

Jiang

2021

Preprint

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Background: Accumulating evidence has suggested that long noncoding RNA (lncRNA) played crucial roles in the development of human malignances including breast cancer. SNHG19 is a newly identified lncRNA which exerted oncogenic function in non-small cell lung cancer, but whether SNHG19 was involved the development of other cancer, such as breast cancer still unclear. Methods: qRT-PCR was performed to examine the expression of SNHG19 and miR-299-5p in breast cancer tissues and cell lines. Cell proliferation was measure using CCK-8 and colony formation assay. Cell migration and invasion ability was detected by wound healing assay and transwell invasion assay. Bioinformatics analysis, dual luciferase reporter assay, RIP assay and Pull down assay were used to verify the direct binding between SNHG19 and miR-299-5p. The xenotransplantation mouse model was established to explore the effect of SNHG19 on breast cancer tumor growth in vivo.Results: We found that SNHG19 expression level was up-regulated in breast cancer tissues and cell lines, while miR-299-5p expression was down-regulated in breast cancer tissues and it was negatively correlated with SNHG19 expression. Silence of SNHG19 inhibited breast cancer cells proliferation, migration and invasion in vitro. Moreover, SNHG19 knockdown suppressed tumor growth of breast cancer cells in vivo. Mechanistically, SNHG19 acted as a ceRNA (competitive endogenous RNA) to sponge miR-299-5p. Finally, the rescue assays further confirmed that miR-299-5p inhibitor reversed the inhibitory effects of SNHG19 knockdown on breast cancer cell proliferation, migration and invasion.Conclusions: In conclusion, our findings proved that SNHG19 promoted breast cancer progression via sponging miR-299-5p and might function as promising prognostic indicator and therapeutic target for breast cancer.

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Section: Discussionmentioning

confidence: 97%

Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p

Jiang

2021

Preprint

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“…Differentiation antagonizing non-protein-coding RNA (DANCR) targets and regulates miR-758-3p, and its overexpression can attenuate the anti-cancer effect of miR-758-3p on breast cancer cells ( 31 ). The rescue of miR-299-5p expression inhibits breast cancer cell migration and invasion, while the inhibition of miR-299-5p promotes cell migration and invasion ( 32 ). Through correlation, expression, and survival analyses, miR-410-3p was found to be the most significant miRNA tumor suppressor upstream of EMC2 .…”

Section: Discussionmentioning

confidence: 99%

The ncRNA-Mediated Overexpression of Ferroptosis-Related Gene EMC2 Correlates With Poor Prognosis and Tumor Immune Infiltration in Breast Cancer

et al. 2021

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Ferroptosis is an iron-dependent programmed cell death process. Although ferroptosis inducers hold promising potential in the treatment of breast cancer, the specific role and mechanism of the ferroptosis-related gene EMC2 in breast cancer have not been entirely determined. The potential roles of EMC2 in different tumors were explored based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), Gene Expression Profiling Interactive Analysis 2 (GEPIA2), Tumor Immune Estimation Resource (TIMER), Shiny Methylation Analysis Resource Tool (SMART), starBase, and cBioPortal for cancer genomics (cBioPortal) datasets. The expression difference, mutation, survival, pathological stage, DNA methylation, non-coding RNAs (ncRNAs), and immune cell infiltration related to EMC2 were analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed to identify the differences in biological processes and functions among different related genes. The expression levels of core prognostic genes were then verified in breast invasive carcinoma samples using immunohistochemistry and breast invasive carcinoma cell lines using real-time polymerase chain reaction. High expression levels of EMC2 were observed in most cancer types. EMC2 expression in breast cancer tissue samples correlated with poor overall survival. EMC2 was mutated and methylated in a variety of tumors and affected survival. The LINC00665-miR-410-3p axis was identified as the most potential upstream ncRNA-related pathway of EMC2 in breast cancer. EMC2 levels were significantly positively correlated with tumor immune cell infiltration, immune cell biomarkers, and immune checkpoint expression. Our study offers a comprehensive understanding of the oncogenic roles of EMC2 across different tumors. The upregulation of EMC2 expression mediated by ncRNAs is related to poor prognosis and tumor immune infiltration in breast cancer.

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“…The copyright holder for this preprint this version posted May 20, 2021. ; https://doi.org/10.1101/2021.05.18.444694 doi: bioRxiv preprint SYT13 in breast cancer (TCGA-BRCA), an oncogenic gene in different cancers (116,117,(122)(123)(124)(125)(126)(127).…”

Section: Discussionmentioning

confidence: 99%

“…While the role of the canonical miR-381-3p is broadly acknowledged as a tumor suppressor (107,108), particularly in breast cancer (112,115), very little is known about the edited form in cancer (44,46). We investigate SYT13 in breast cancer (TCGA-BRCA), an oncogenic gene in different cancers (116,117,(122)(123)(124)(125)(126)(127).…”

Section: Discussionmentioning

confidence: 99%

A concurrent canonical and modified miRNAome pan-cancer study on TCGA and TARGET cohorts leads to an enhanced resolution in cancer

Distefano

Tomasello

Vinciguerra

et al. 2021

Preprint

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MiRNA Epitranscriptomics has placed a new layer of complexity in the cancer field. Despite miRNA editing and shifted miRNA isoforms are gaining attention due to recent improvements in next-generation sequencing, a simultaneous study of both modifications in cancer is still missing. Here, we concurrently profiled multiple miRNA modifications, such as A-to-I RNA editing and shifted miRNA isoforms, in >13K adult and pediatric tumor samples across 38 distinct cancer cohorts from The Cancer Genome Atlas and The Therapeutically Applicable Research to Generate Effective Treatments datasets. We investigated the differences among canonical miRNAs and a wider comprehensive miRNAome from the expression, clustering, dysregulation, and prognostic perspective. Interestingly, the wider miRNAome boosted clustering results, uniquely outlining cohorts' clinical-pathological features. The abundance of expressed miRNA isoforms directly related to the activation/deactivation of critical carcinogenesis pathways. We found dysregulated modified miRNAs characterized by an opposite expression trend than their canonical counterparts in cancer, potentially impacting their targetome and function. Our study emphasizes the importance of modified miRNAs as potential cancer biomarkers and gene expression regulators, outlining once more the importance of going beyond the well-established paradigm of one-mature-miRNA per miRNA arm.

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MicroRNA‑299‑5p inhibits cell metastasis in breast cancer by directly targeting serine/threonine kinase 39

Cited by 19 publications

References 42 publications

Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p

Long Non-Coding RNA SNHG19 Promotes Breast Cancer Progression by Regulating miR-299-5p

The ncRNA-Mediated Overexpression of Ferroptosis-Related Gene EMC2 Correlates With Poor Prognosis and Tumor Immune Infiltration in Breast Cancer

A concurrent canonical and modified miRNAome pan-cancer study on TCGA and TARGET cohorts leads to an enhanced resolution in cancer

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