MicroRNA-29a Functions as a Tumor Suppressor and Increases Cisplatin Sensitivity by Targeting NRAS in Lung Cancer

Liu, Xin; Lv, Xianping; Yang, Qiankun; Jin, Huifang; Zhou, Wenpeng; Fan, Qingxia

doi:10.1177/1533033818758905

Cited by 32 publications

(26 citation statements)

References 32 publications

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“…Our data showed that circulating plasma miRNA-17 and miRNA-222 expression levels were significantly higher in patients with NSCLC than those in healthy controls (P=0.000). In accordance with our findings, previous studies have shown that aberrant miRNA expression in NSCLC patients' blood and healthy individuals is powerfully associated with cancer development and progress (Xu et al, 2015;Zaporozhchenko et al, 2018;Yang et al, 2018;Liu et al, 2018;Liu and Wang, 2017). Contrary to such speculations, Li et al, (2013) have revealed that the expression of miR-17-5p is reduced in triple-negative breast cancer tissues and that it can suppress tumor progression through inhibition of ETV1 oncogene in triple-negative breast cancer cells.…”

Section: Discussionsupporting

confidence: 93%

Assessment of Circulating miRNA-17 and miRNA-222 Expression Profiles as Non-Invasive Biomarkers in Egyptian Patients with Non-Small-Cell Lung Cancer

Hetta

Zahran²,

El‐Mahdy

et al. 2019

Asian Pac J Cancer Prev

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Background: Lung cancer is one of the main human health threats. Survival of lung cancer patients depends on the timely detection and diagnosis. Among the genetic irregularities that control cancer development and progression, there are microRNAs (miRNAs). This study aimed to assess the plasma level of circulating miRNA-17 and miRNA-222 as non-invasive markers in non-small-cell lung cancer (NSCLC) patients. Patients and methods: A total of 40 patients with NSCLC and 20 healthy controls who were matched in terms of age and sex with the patient group were included in this case-control study.. Estimation of miRNA-17 and miRNA-222 expression profiles in the plasma was done using quantitative real-time PCR (qRT-PCR). The relationship between both markers and their clinicopathological features were also determined. Receiver operating characteristic (ROC) curve analysis was done to evaluate the role of these microRNAs in NSCLC diagnosis and follow-up. Results: MiRNA-17 and miRNA-222 levels were significantly upregulated in NSCLC patients compared with controls (48.32±12.35 vs 1.16±0.19 and 34.53±3.1 vs 1.22±0.14) (P=0.000). Plasma miRNA-17 level was increased, and the miRNA-222 level was decreased across different stages of the disease; however, these differences d were not statistically significant (P=0.4, P=0.5, respectively). The miRNA-17 levels were higher in the lung cancer patients with metastasis , but miRNA-222 levels were lower patients without metastasis. We found no statistically significant difference in this regard(P=0.4 vs P=0.3, respectively). ROC curve analysis showed that the sensi¬tivity and specificity of miRNA-17 were 77.78% and 87.50% , and of miRNA-222 were 50% and 88.89%. Conclusion: MiRNA-17 and miRNA-222 can be considered as non-invasive biomarkers for detection of early lung carcinogenesis and metastasis in patients with NSCLC, hence providing a basis for the development of novel therapeutic approaches.

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Section: Discussionsupporting

confidence: 93%

Assessment of Circulating miRNA-17 and miRNA-222 Expression Profiles as Non-Invasive Biomarkers in Egyptian Patients with Non-Small-Cell Lung Cancer

Hetta

Zahran²,

El‐Mahdy

et al. 2019

Asian Pac J Cancer Prev

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“…in addition, mir-29a was predicted to interact with circ_087212. mir-29a was identified to regulate malignant cell features and drug resistance in several cancer types, including hepatocellular cancer and lung cancer by targeting Sirtuin 1 and nraS (15,28). Based on the present results, identifying novel approaches that may target and affect the stability of the interaction between circrna and mirnas may be important for inhibiting cancer metastasis, invasiveness and recurrence.…”

Section: Discussionmentioning

confidence: 62%

Circular RNA expression profiling identifies specific circular RNAs in tongue squamous cell carcinoma

Wei

et al. 2020

Mol Med Report

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Tongue squamous cell carcinoma (TScc) is the most frequent type of oral cancer associated with high malignancy. circular rnas (circrnas) are a form of non-coding rna with stable and conserved expression in mammalian cells. The aim of the present study was to investigate circRNAs expression profiles in TSCC, and examine the roles and potential mechanisms of circrna-081069 (circ_081069). a high-throughput circrna microarray analysis of tumor samples and adjacent normal tissues from four patients with TScc was performed. Bioinformatic analysis was conducted to screen the differentially expressed circrnas. reverse transcription-quantitative PCR was performed to confirm the microarray results. a migration assay and proliferation assay were performed to detect the migratory and proliferative ability of TScc cells. a luciferase assay was conducted to investigate the interaction between circ_081069 and microrna (mirna/mir)-665. in total, 335 circrnas were found to be differentially expressed in tumor tissues. among them, 59 were upregulated and 276 were downregulated (P<0.05; fold change ≥2 or ≤0.5). A total of seven circRNAs, including two upregulated and five downregulated circRNAs, were further confirmed using quantitative PCR analysis in the ten paired TScc tissues and adjacent normal tissues. The present study showed that circrna_081069 promoted the migratory and proliferative ability of TScc cells in vitro. Furthermore, the potential circrna-mirna interactions were predicted, and the present results identified miR-665 as a miRNA target of circ_081069. The present results suggested that circrnas may be involved in TScc development, and understanding the interaction between circ_081069 and mir-665 may facilitate the development of novel diagnostic and therapeutic targets for TScc.

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“…MicroRNAs (miRNAs) were a quantity of short non-coding RNAs that could inhibit the function of target genes through degrading the mRNA or suppressing its translation in post-transcriptional regulation 4,5. MiR-29a has been reported to be a tumor suppressors and was participated in the proliferation of glioma and lung cancer 6,7. Xiong et al8 revealed that miR-29a inhibited the growth and metastasis through targeting BMI1 in melanoma.…”

Section: Introductionmentioning

confidence: 99%

<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

Peng

Niu

Wang

et al. 2019

OTT

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IntroductionCervical cancer is the second most frequently malignant tumors in females and metastasis is a challenge of the treatment of cervical cancer. MiR-29a is usually low expressed in several tumors and its functions in cervical cancer remain unclear.Patients and methodsThe quantitative real-time polymerase chain reaction was employed to assess the expression of miR-29a and the Sirtuin-1 (SIRT1). Cell metastatic ability was assessed using Transwell and Western blot assays. The dual-luciferase reporter assay was performed to verify that miR-29a targeted to the 3’-untranslated region (UTR) of SIRT1 mRNA.ResultsMiR-29a was low expressed in cervical cancer and downregulation of miR-29a was associated with poor outcome. MiR-29a regulated the expression of SIRT1 by targeting to its 3’-UTR of mRNA in HeLa cells. SIRT1 was upregulated in cervical cancer tissues and cells in comparison with the non-tumor tissues and normal cells. Upregulation of SIRT1 predicted worse outcome of cervical cancer patients. MiR-29a was participated in the migration, invasion and epithelial–mesenchymal transition (EMT) in cervical cancer through directly targeting to the 3’-UTR of SIRT1 mRNA. SIRT1 reversed partial roles of miR-29a on metastasis in cervical cancer.ConclusionmiR-29a suppressed migration, invasion and EMT by directly targeting to SIRT1 in cervical cancer. The newly identified miR-29a/SIRT1 axis provides novel insight into the pathogenesis of cervical cancer.

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MicroRNA-29a Functions as a Tumor Suppressor and Increases Cisplatin Sensitivity by Targeting NRAS in Lung Cancer

Cited by 32 publications

References 32 publications

Assessment of Circulating miRNA-17 and miRNA-222 Expression Profiles as Non-Invasive Biomarkers in Egyptian Patients with Non-Small-Cell Lung Cancer

Assessment of Circulating miRNA-17 and miRNA-222 Expression Profiles as Non-Invasive Biomarkers in Egyptian Patients with Non-Small-Cell Lung Cancer

Circular RNA expression profiling identifies specific circular RNAs in tongue squamous cell carcinoma

<p>MiR-29a function as tumor suppressor in cervical cancer by targeting SIRT1 and predict patient prognosis</p>

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