MicroRNA‑30a suppresses papillary thyroid cancer cell proliferation, migration and invasion by directly targeting E2F7

Abstract

microRNA (miRNA/miR)-30a, a tumor-associated miRNA, has been implicated in the tumorigenesis and progression of different types of human cancer. Thyroid cancer is a common endocrine malignancy, of which papillary thyroid cancer (PTC) accounts for ~80-90% of all TC. However, the effect of miR-30a in PTC is yet to be fully elucidated. The TPC-1 human PTC cell line, as well as the normal human thyroid cell line (HT-ori3), were utilized in the current study. The PTC cell line was transfected with a miR-30a mimic. Subsequently, reverse transcription-quantitative polymerase chain reaction was performed to detect the expression of miR-30a and E2F transcription factor 7 (E2F7). Cell proliferation was assessed via a MTT assay and transwell migration and invasion assays were performed to detect the migration and invasion of PTC cells. A dual-luciferase reporter assay was also utilized to clarify the association between miR-30a and E2F7. The results of the current study revealed that miR-30a was significantly downregulated in TPC-1 cells compared with HT-ori3 cells and that the expression of E2F7 was significantly upregulated in PTC cells. The upregulation of miR-30a also inhibited the proliferation, migration and invasion of PTC cells. Furthermore, the luciferase assay revealed that miR-30a binds to the 3'-UTR of E2F7. Additionally, the overexpression of miR-30a decreased E2F7 levels in TPC-1 cells. These results indicate that miR-30a functions as a tumor suppressor in PTC by direct targeting E2F7 and that miR-30a may be a novel therapeutic target for patients with PTC.

Novel Potential Therapeutic Target for E2F1 and Prognostic Factors of E2F1/2/3/5/7/8 in Human Gastric Cancer

“…E2F7 has been reported in a variety of tumors. 47 , 48 Zhang et al. 49 have analyzed the relationships between E2Fs and the prognosis of GC.…”

“…E2F7 has been reported in a variety of tumors. 47,48 Zhang et al 49 have analyzed the relationships between E2Fs and the prognosis of GC. They believed that the mRNA level of E2F7 was related to GC cell invasion and tumor differentiation.…”

Effect of miR‑205 on proliferation and migration of thyroid cancer cells by targeting CCNB2 and the mechanism

“…Jiao et al (15) found that ZEB1 overexpression reverses the inhibitory effect of miR-873 overexpression on the proliferation and invasion of TC cells, and miR-873 may play a tumor inhibitory role in the development of TC by inhibiting ZEB1. Guo and Zhang (16) suggest that miR-30a plays a role in inhibiting tumor growth in TC by directly targeting the E2F7 gene. miR-30a may be a new therapeutic target for TC.…”

MicroRNA in Papillary Thyroid Carcinoma: A Systematic Review from 2018 to June 2020

“…Overexpression of microRNA-150-5p regulated cell proliferation, metastasis, and apoptosis by regulating BRAFV600E [94]. Both the IGF1R [139] and E2F7 [32] genes are targeted directly by the microRNA-30a. MicroRNA-203 inhibits proliferation and motility, and induces apoptosis of PTC cells via regulation of the expression of Bcl-2 [91], and suppresses EMT, invasion, proliferation, and migration of PTC cells via downregulated AKT3 [101].…”