MicroRNA-31 Can Positively Regulate the Proliferation, Differentiation and Migration of Keratinocytes

Wang, Fei; Gao, Yuantao; Yuan, Yitong; Du, Ruochen; Li, Pengfei; Liu, Fang; Wang, Yali; Zhang, Ruxin; Zhao, Bichun; Wang, Chunfang

doi:10.1159/000508612

“…The level of circulating EVs expressing IL-17A was higher in patients with moderate-to-severe psoriasis than in those with mild psoriasis ( Jacquin-Porretaz et al, 2019 ). In comparison to healthy donors (HD), miRNAs of EVs from psoriasis patients showed an altered expression ( Pasquali et al, 2020 ; Wang et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Lipidic Profile Changes in Exosomes and Microvesicles Derived From Plasma of Monoclonal Antibody-Treated Psoriatic Patients

Paolino¹,

Buratta

²

,

Mercuri³

et al. 2022

Front. Cell Dev. Biol.

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Psoriasis is a chronic immune-mediated inflammatory skin disorder affecting children and adults. To date no approved biomarkers for diagnosis of this disease and follow up of patients have been translated into clinical practice. Recently, extracellular vesicles (EVs) secreted by all cells and present in almost all biological fluids are playing a crucial role in diagnosis and follow up of several diseases, including psoriasis. Since many psoriatic patients show altered plasma lipid profiles and since EVs have been involved in psoriasis pathogenesis, we studied the phospholipid profile of EVs, both microvesicles (MV) or exosomes (Exo), derived from plasma of psoriatic patients undergoing systemic biological treatment (secukinumab, ustekinumab, adalimumab), in comparison with EVs of untreated patients and healthy donors (HD). EVs were evaluated by immune electronmicroscopy for their morphology and by NanoSight for their amount and dimensions. EV phospholipid profiling was performed by High Resolution Liquid Chromatography-Mass Spectrometry and statistical Partial Least Squares Discriminant Analysis. Our results demonstrated that psoriatic patients showed a higher concentration of both MV and Exo in comparison to EVs from HD. The phospholipid profile of Exo from psoriatic patients showed increased levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol and lysoPC compared to Exo from HD. Sphingomyelin (SM) and phosphatidylinositol (PI) are the only phospholipid classes whose levels changed in MV. Moreover, the therapy with ustekinumab seemed to revert the PE and PC lipid composition of circulating Exo towards that of HD and it is the only one of the three biological drugs that did not alter SM expression in MV. Therefore, the determination of lipid alterations of circulating EVs could harbor useful information for the diagnosis and drug response in psoriatic patients.

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“…In addition, it being able to improve the inflammatory process occurring during healing due to the release of inflammatory cytokines [33,34]. The miR-31 family is also closely related to the wound healing process, these miRNAs can regulate keratinocytes proliferation, differentiation, and migration through the regulation of the signaling pathways NF-κB, RAS/MAPK, Notch, and cytokines [35,36]. The miR-31-5p was found with expression decreased on day 1 and increased on day 5.…”

Section: Plos Onementioning

confidence: 99%

MicroRNAs expressed during normal wound healing and their associated pathways: A systematic review and bioinformatics analysis

Azevedo

¹

,

Giorgi

²

,

Nedel

³

et al. 2023

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MicroRNAs (miRNAs) are responsible for regulating gene expression post-transcriptionally. Are involved in several biological processes, such as wound healing. Understanding the miRNAs involved in this process is fundamental for the development of new therapies. So, due to the need to understand the role of these molecules, we aimed systematically review the literature in order to identify which miRNAs are involved in the wound healing and determine, through bioinformatics analysis, which signaling pathways are associated with these miRNAs. An electronic search was performed in the following databases: National Library of Medicine National Institutes of Health (PubMed), Science Direct, Scifinder, Scopus and Web of Science, using the descriptors: “(microRNA [MeSH])” and “(skin [MeSH])” and “(wound healing [MeSH])”. After the search, two independent and previously calibrated reviewers selected the articles that analyzed the expression pattern of miRNAs in wound healing in in vivo studies, using the software Zotero bibliography manager. Following, bioinformatic analysis was performed using the software DIANA Tools, mirPath v.3 and the data was interpreted. The bioinformatics analysis revealed that on the day 1 there were 13 union pathways, eight of which were statistically significant. Still on the day 1, among the miRNAs that had a decrease in their expression, 12 of 17 union pathways found were statistically significant. On the day 5, among the miRNAs with an increase in expression, 16 union pathways were found, 12 of which were statistically significant. Finally, among the miRNAs with decreased expression, 11 of 15 union pathways found were statistically significant. Although it has been found substantial heterogeneity in the studies, with this systematic review, it was possible to study the panorama of miRNAs that may be altered in the wound healing. The present review summarizes existing evidence of miRNAs associated to wound healing, and these findings can contribute to new therapeutic approaches.

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“…The small copies of microRNA-31 can be attempted to explain the reciprocal dysregulation of the transcription factors in patients with CIDP. Several studies demonstrate that miR-31 can positively regulate cell proliferation, differentiation, and activity by regulating NF- κ B, RAS/MAPK, Notch, and some cytokine signalling pathways [ 12 , 14 , 27 , 28 ]. The increased miRNA-31 copies in our patients treated with IVIg may be due to the mechanism of action of IVIg.…”

Section: Discussionmentioning

confidence: 99%

miR-31-5p as a Potential Circulating Biomarker and Tracer of Clinical Improvement for Chronic Inflammatory Demyelinating Polyneuropathy

Dziadkowiak

¹

,

Baczyńska

²

,

Wieczorek

³

et al. 2023

Oxidative Medicine and Cellular Longevity

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Background. MicroRNAs are endogenous, small noncoding RNA molecules that play a pivotal role in the regulation of gene expression. MicroRNAs are involved in many biological processes such as proliferation, cell differentiation, neovascularization, and apoptosis. Studies on microRNA expression may contribute to a better understanding of the pathomechanism of chronic inflammatory demyelinating polyneuropathy (CIDP) and consequently enable the development of new therapeutic measures using antisense miRNAs (antagomirs). In this study, we evaluated the level of miR-31-5p in the serum of patients with CIDP and its correlation with the miR-31-5p level and clinical presentation and electrophysiological and biochemical parameters. Methods. The study group consisted of 48 patients, mean age 61.60 ± 11.76 , who fulfilled the diagnostic criteria of a typical variant of CIDP. The expression of miR-31-5p in patient serum probes was investigated by droplet digital PCR. The results were correlated with neurophysiological findings and the patient’s clinical and biochemical parameters. Results. The mean copy number of miRNA-31 in 100 μl serum was 1288.64 ± 2001.02 in the CIDP group of patients, while in the control group, it was 3743.09 ± 4026.90 . There was a significant positive correlation (0.426) between IgIV treatment duration and miR-31-5p expression. Patients without IgIV treatment showed significantly lower levels of miR-31 compared to the treated group ( 259.44 ± 304.02 vs. 1559.48 ± 2168.45 ; p = 0.002 ). The group of patients with body weight > 80 kg showed statistically significantly lower levels of miRNA-31-5p than the patients with lower body weight ( 934.37 ± 1739.66 vs. 1784.62 ± 2271.62 , respectively; p = 0.014 ). Similarly, the patients with elevated cerebrospinal fluid (CSF) protein levels had significantly higher miRNA-31-5p expression than those with normal protein levels ( 1393.93 ± 1932.27 vs. 987.38 ± 2364.10 , respectively; p = 0.044 ). Conclusion. The results may support the hypothesis that miR-31-5p is strongly involved in the autoimmune process in CIDP. The positive correlation between higher miR-31-5p levels and duration of IVIg treatment may be an additional factor explaining the efficacy of prolonged IVIg therapy in CIDP.

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MicroRNA-31 Can Positively Regulate the Proliferation, Differentiation and Migration of Keratinocytes

Cited by 8 publications

References 62 publications

Lipidic Profile Changes in Exosomes and Microvesicles Derived From Plasma of Monoclonal Antibody-Treated Psoriatic Patients

Lipidic Profile Changes in Exosomes and Microvesicles Derived From Plasma of Monoclonal Antibody-Treated Psoriatic Patients

MicroRNAs expressed during normal wound healing and their associated pathways: A systematic review and bioinformatics analysis

miR-31-5p as a Potential Circulating Biomarker and Tracer of Clinical Improvement for Chronic Inflammatory Demyelinating Polyneuropathy

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