MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Gao, Jiarong; Shi, Miao Miao; Jiang, Hui; Zhu, Xiaoqing; Wei, Liang; Qin, Xiu Juan

doi:10.1007/s00210-022-02261-z

Cited by 6 publications

(1 citation statement)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mouse SV40-MES-13 GMCs were purchased from BNCC Biological Technology and cultured in DMEM (Beijing Solarbio Science & Technology Co., Ltd.) supplemented with 10% FBS (Biological Industries Sartorius AG) and penicillin/streptomycin at 37˚C in a humidified, 5% CO 2 atmosphere. GMCs stimulated with 3.0 µg/ml LPS (MilliporeSigma) (10)(11) were defined as the model group (n=3) and unstimulated GMCs were defined as the control group (n=3).…”

Section: Methodsmentioning

confidence: 99%

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

et al. 2023

View full text Add to dashboard Cite

Long non-coding RNAs (lncRNAs) are ncRNA transcripts >200 nucleotides that are important genetic regulators. LncRNAs can directly regulate mRNA through a lncRNA-mRNA regulatory mode and can also regulate mRNA through competitive binding to micro (mi)RNA, which is generally known as the competitive endogenous RNA (ceRNA) network. The present study evaluated the functional roles and regulatory networks of lncRNAs in chronic glomerulonephritis (CGN). The proliferative ability of mouse glomerular mesangial cells (GMCs) induced by different concentrations of lipopolysaccharide (LPS) was assessed using the Cell Counting Kit-8 assay, and RNA sequencing (RNA-seq) was performed to identify differentially expressed lncRNAs in LPS-induced GMCs. Based on the sequencing results, six lncRNAs were selected for validation using reverse transcription-quantitative PCR (RT-qPCR). Furthermore, the lncRNA-mRNA regulatory network and the lncRNA-miRNA-mRNA ceRNA network were constructed to assess the role and mechanism of CGN-related lncRNAs. To elucidate the biological functions of lncRNAs, Gene Ontology (GO) biological process term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed on all mRNAs involved in the lncRNA-mRNA regulatory network and in the ceRNA network. A total of 1,532 differentially expressed lncRNAs, including 594 upregulated lncRNAs and 938 downregulated lncRNAs, were identified using RNA-seq.The results of RT-qPCR validation were consistent with RNA-seq results. An lncRNA-mRNA regulatory network, including 236 lncRNAs and 556 mRNAs, and a ceRNA network, including 6 lncRNAs, 18 miRNAs and 419 mRNAs, were successfully constructed. The GO biological process term enrichment and KEGG pathway enrichment analyses demonstrated that those lncRNAs were often related to inflammatory response and substance metabolism. The present study identified key CGN-related lncRNAs in LPS-induced GMCs, and further demonstrated a global view of the lncRNA-mRNA regulatory network and ceRNA network involved in CGN. These results offered novel insights into the roles of lncRNAs in the pathogenesis of CGN and identified potential diagnostic biomarkers.

show abstract

Section: Methodsmentioning

confidence: 99%

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

et al. 2023

View full text Add to dashboard Cite

show abstract

The potential role of N6-methyladenosine modification of LncRNAs in contributing to the pathogenesis of chronic glomerulonephritis

et al. 2023

View full text Add to dashboard Cite

RNA sequencing reveals the mechanism of FTO in inhibiting inflammation and excessive proliferation of lipopolysaccharide-induced human glomerular mesangial cells

Zhuang

Liu

Wei

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Cited by 6 publications

References 43 publications

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

The potential role of N6-methyladenosine modification of LncRNAs in contributing to the pathogenesis of chronic glomerulonephritis

RNA sequencing reveals the mechanism of FTO in inhibiting inflammation and excessive proliferation of lipopolysaccharide-induced human glomerular mesangial cells

Contact Info

Product

Resources

About

MicroRNA-339-5p inhibits lipopolysaccharide-induced rat mesangial cells by regulating the Syk/Ras/c-Fos pathway

Cited by 6 publications

References 43 publications

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑­miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑­miRNA‑mRNA ceRNA network by bioinformatics analysis

The potential role of N6-methyladenosine modification of LncRNAs in contributing to the pathogenesis of chronic glomerulonephritis

RNA sequencing reveals the mechanism of FTO in inhibiting inflammation and excessive proliferation of lipopolysaccharide-induced human glomerular mesangial cells

Contact Info

Product

Resources

About

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis

Construction of chronic glomerulonephritis‑related lncRNA‑mRNA regulatory network and lncRNA‑miRNA‑mRNA ceRNA network by bioinformatics analysis