microRNA-34 family: From mechanism to potential applications

Wang, Chen; Jia, Qiaojin; Guo, Xiaojun; Li, Ke; Chen, Wujian; Shen, Quan; Xu, Chenggang; Fu, Yuejun

doi:10.1016/j.biocel.2022.106168

Cited by 22 publications

(16 citation statements)

References 182 publications

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“…3.1 Expression of miR-34-5p was inhibited in Sf9 cells and larvae after AcMNPV infection miR-34-5p is highly conserved among multiple species, such as arthropods, nematodes and mammals. 31 The miR-34-5p sequences of various species were obtained from the miRBase online database and a multi-sequence alignment was performed. The high homology of miR-34-5p is shown in Figure S1.…”

Section: Resultsmentioning

confidence: 99%

“…36 miR-34-5p is highly conserved among multiple species, such as arthropods, nematodes and mammals. 31 In arthropods and nematodes, miR-34-5p is involved in aging, stress responses, neurodevelopment, insect immunity and development. B. mori, miR-34-5p can regulate the growth and wing formation of larvae by targeting BmE74 and BmCPG4, and directly regulating molt signals and stratum corneum proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Host‐encoded miRNAs not only regulate the cellular processes, but also have an important role against pathogen infection 36 . miR‐34‐5p is highly conserved among multiple species, such as arthropods, nematodes and mammals 31 . In arthropods and nematodes, miR‐34‐5p is involved in aging, stress responses, neurodevelopment, insect immunity and development.…”

Section: Discussionmentioning

confidence: 99%

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microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

Jia

2022

Pest Management Science

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BACKGROUND Spodoptera frugiperda is one of the significant migratory pests in the Global Alert issued by the Food and Agriculture Organization of the United Nations. As an insect‐specific microbial insecticide, baculovirus has been used to control various pests. MicroRNA‐34‐5p (miR‐34‐5p) is involved in regulating growth, reproduction and innate immunity to pathogens in insects, playing an essential role in host–virus interactions. In this study, we explored the critical function of miR‐34‐5p encoded by S. frugiperda in the anti‐Autographa californica multiple nucleopolyhedrovirus (AcMNPV), providing a reference for the design of a miR‐34‐5p target biopesticide against S. frugiperda and a theoretical basis for the wide application of microRNAs (miRNAs) in green pest control technology. RESULTS We focused on miR‐34‐5p identified as downregulated in Sf9 cells and S. frugiperda larvae infected by AcMNPV. The regulatory function of miR‐34‐5p in AcMNPV–S. frugiperda interactions was studied by transfecting synthetic mimics and inhibitors, and constructing recombinant bacmids with miR‐34‐5p overexpression. miR‐34‐5p inhibited the production of infectious budded virions at the cellular and insect levels, inhibited the replication of the viral DNA and glucose metabolism, and increased the transcription of the antimicrobial peptide gloverin. Furthermore, the virus genes odv‐e66, ac78 and ie2 were shown to be direct targets. CONCLUSION We systematically revealed the mechanism by which miR‐34‐5p is involved in the insect antiviral process. miR‐34‐5p inhibited the replication and infection of AcMNPV by directly targeting AcMNPV genes, especially ac78 and ie2. Our study provides a new direction and thinking for the prevention and green control of lepidopteran pests. © 2022 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

Jia

2022

Pest Management Science

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“…From this cluster, miR-34c-3p and miR-200c-3p exhibited continuous upregulation from early to late juvenile to adult period. MicroRNA-34 is highly conserved in evolution and its miR-34 family has been identified to be pivotal in organ development, organismal or organ senescence, stress response, spermatogenesis and signal transduction ( Wang et al, 2022 ). In goat testis, miR-34c has been shown to promote apoptosis and suppress proliferation of SSCs ( Li et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Feline microRNAome in ovary and testis: Exploration of in-silico miRNA-mRNA networks involved in gonadal function and cellular stress response

Amelkina

Silva

et al. 2022

Front. Genet.

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The aim of the study was to perform the first in-depth analysis of miRNAs in ovarian and testicular tissues of the domestic cat, a critical biomedical model. Specifically, potential miRNA involvement was explored in gonadal function, testis development, and cellular stress response to preservation protocols. We performed miRNA-sequencing on 20 ovarian and 20 testicular samples from 15 cats, including different ages and tissue treatments. Using fresh tissues (n = 15), we confirmed gonadal expression of 183 miRNA precursors and discovered additional 52 novel feline candidate precursors. We integrated the mRNA data from our previous study on the same age and treatment groups to create in-silico miRNA-mRNA networks and their functional enrichment, which allows comprehensive exploration into possible miRNA functions in cat gonads. Clusters of miRNAs united by shared differentially expressed mRNA targets are potentially involved in testicular development and spermatogenesis. MicroRNAs could play a significant role in ovarian tissue response to stress from microwave-assisted dehydration, with smaller roles in cellular response to vitrification in both ovary and testis. This new list of miRNAs with potential function in cat gonads is a major step towards understanding the gonadal biology, as well as optimizing fertility preservation protocols.

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“…MiRNAs directly interact with partially complementary target sites located in the 3′ untranslated region of target mRNAs, repressing their expression, and thus their main function is gene regulation by the inhibition of translation or by targeting mRNAs for degradation [ 22 ]. A well-known example of miRNA is the miR-34 family, which is a multifunctional miRNA family that has been demonstrated to be important in organ development, organ senescence, stress response, aging, neurodevelopment, signal transduction, and most importantly, human fertility [ 23 , 24 ]. Other examples of miRNAs modulating human fertility include the MiR-888-3p, which was found to be significantly overexpressed in AZ patients [ 25 ], and MiR-27a and -27b.…”

Section: Introductionmentioning

confidence: 99%

Further Insights on RNA Expression and Sperm Motility

Silva

Viana

Barros

et al. 2022

Genes

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Asthenozoospermia is one of the main causes of male infertility and it is characterized by reduced sperm motility. Several mutations in genes that code for structural or functional constituents of the sperm have already been identified as known causes of asthenozoospermia. In contrast, the role of sperm RNA in regulating sperm motility is still not fully understood. Consequently, here we aim to contribute to the knowledge regarding the expression of sperm RNA, and ultimately, to provide further insights into its relationship with sperm motility. We investigated the expression of a group of mRNAs by using real-time PCR (CATSPER3, CFAP44, CRHR1, HIP1, IQCG KRT34, LRRC6, QRICH2, RSPH6A, SPATA33 and TEKT2) and the highest score corresponding to the target miRNA for each mRNA in asthenozoospermic and normozoospermic individuals. We observed a reduced expression of all mRNAs and miRNAs in asthenozoospermic patients compared to controls, with a more accentuated reduction in patients with progressive sperm motility lower than 15%. Our work provides further insights regarding the role of RNA in regulating sperm motility. Further studies are required to determine how these genes and their corresponding miRNA act regarding sperm motility, particularly KRT34 and CRHR1, which have not previously been seen to play a significant role in regulating sperm motility.

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microRNA-34 family: From mechanism to potential applications

Cited by 22 publications

References 182 publications

microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

microRNA‐34‐5p encoded by Spodoptera frugiperda regulates the replication and infection of Autographa californica multiple nucleopolyhedrovirus by targeting odv‐e66, ac78 and ie2

Feline microRNAome in ovary and testis: Exploration of in-silico miRNA-mRNA networks involved in gonadal function and cellular stress response

Further Insights on RNA Expression and Sperm Motility

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