MicroRNA-342-3p is a potent tumour suppressor in hepatocellular carcinoma

Komoll, Ronja-Melinda; Hu, Qing; Olarewaju, Olaniyi; Döhlen, Lena von; Yuan, Qing; Xie, Yu; Tsay, Hsin-Chieh; Daon, Joel; Qin, Renyi; Manns, Michael P.; Sharma, Amar Deep; Goga, Andrei; Ott, Michael; Balakrishnan, Asha

doi:10.1016/j.jhep.2020.07.039

Cited by 131 publications

(88 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MiRNAs are known to play important roles in the progression of multiple diseases, including liver cancer (17,27). Our research found miR-513a-5p antagomir partially reversed the inhibitory effect of LINC01234 knockdown on liver cancer, further verified the function of miR-513a-5p in liver cancer progression.…”

Section: Discussionsupporting

confidence: 73%

Knockdown of lncRNA LINC01234 Suppresses the Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

Song

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

Background: Liver cancer is a frequent malignancy with poor prognosis and high mortality all over the world. It has been reported many lncRNAs could modulate the tumorigenesis of liver cancer. To identify novel potential targets for liver cancer, the differential expressed lncRNAs between liver cancer and adjacent normal tissues was analyzed with bioinformatics tool. Methods: The differential expressed lncRNAs between liver cancer and adjacent normal tissues were analyzed with bioinformatics tool. Cell viability and proliferation was tested by CCK8 and Ki67, respectively. Apoptosis of liver cancer cells was tested by flow cytometry. Gene and protein expressions in liver cancer cells were measured by qRT-PCR and western blot, respectively. In vivo model of liver cancer was established to detect the effect of LINC01234 on liver cancer in vivo. Results: LINC01234 was found to be negatively correlated with the survival rate of patients with liver cancer. Moreover, knockdown of LINC01234 significantly suppressed the proliferation and invasion of liver cancer cells via inducing the apoptosis. Meanwhile, miR-513a-5p was sponged by LINC01234, and USP4 was found to be a direct target of miR-513a-5p. In addition, LINC01234 knockdown inhibited the tumorigenesis of liver cancer via inactivating TGF-β signaling. Furthermore, silencing of LINC01234 notably inhibited the tumor growth of liver cancer in vivo. Conclusion: Downregulation of LINC01234 could inhibit the tumorigenesis of liver cancer via mediation of miR-513a-5p/USP4/TGF-β axis. Thus, LINC01234 might serve as a new target for the treatment of liver cancer.

show abstract

Section: Discussionsupporting

confidence: 73%

Knockdown of lncRNA LINC01234 Suppresses the Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

Song

et al. 2020

Front. Oncol.

View full text Add to dashboard Cite

show abstract

“…miRNAs are small endogenous non-coding RNAs, which is able to bind to a variety of specific mRNAs to cause gene silencing. Numerous researchers found that the modulation of miRNAs expression in cancer cells is responsible for activate or inhibit tumorigenesis or metastasis (41). Firstly, miRNAs could directly bind to mRNA to suppress transcription activity of downstream oncogenes or tumor suppressor genes.…”

Section: Discussionmentioning

confidence: 99%

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

Chen

Wang

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Pancreatic cancer is a common malignant tumor with a high incidence and mortality rate. The prognosis of patients with pancreatic cancer is considerably poor due to the lack of effective treatment in clinically. Despite numerous studies have revealed that baicalein, a natural product, is responsible for suppressing multiple cancer cells proliferation, motility and invasion. The mechanism by which baicalein restraining pancreatic cancer progression remains unclear. In this study, we firstly verified that baicalein plays a critical role in inhibiting pancreatic tumorigenesis in vitro and in vivo. Then we analyzed the alteration of microRNAs (miRNAs) expression levels in Panc-1 cells incubated with DMSO, 50 and 100 μM baicalein by High-Throughput sequencing. Intriguingly, we observed that 20 and 39 miRNAs were accordingly up- and down-regulated through comparing Panc-1 cells exposed to 100 μM baicalein with the control group. Quantitative PCR analysis confirmed that miR-139-3p was the most up-regulated miRNA after baicalein treatment, while miR-196b-5p was the most down-regulated miRNA. Further studies showed that miR-139-3p induced, miR-196b-5p inhibited the apoptosis of Panc-1 cells via targeting NOB1 and ING5 respectively. In conclusion, we demonstrated that baicalein is a potent inhibitor against pancreatic cancer by modulating the expression of miR-139-3p or miR-196b-5p.

show abstract

“…15 For example, miR-342 has been reported to significantly decrease cell proliferation, migration and colony formation in hepatocellular carcinoma. 16 MiR-342 also caused significant inhibition of the proliferation, colony formation, invasion and migration of breast cancer. 17 The DExD RNA helicases contains a conserved core consisting of nine conserved motifs that include the characteristic D-E-x-D motif, which confer ATP hydrolysis and RNA unwinding activities, establishing it as RNA helicase.…”

Section: Introductionmentioning

confidence: 95%

“…Studies have demonstrated that miRNAs usually play carcinogenic or tumor suppressive roles in multiple cancers 15 . For example, miR‐342 has been reported to significantly decrease cell proliferation, migration and colony formation in hepatocellular carcinoma 16 . MiR‐342 also caused significant inhibition of the proliferation, colony formation, invasion and migration of breast cancer 17 …”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG20 promoted proliferation, invasion and inhibited cell apoptosis of lung adenocarcinoma via sponging miR‐342 and upregulating DDX49

Wang

Zhu

et al. 2020

Thoracic Cancer

View full text Add to dashboard Cite

Background: There is increasing evidence that long non-coding RNA (lncRNA) small nucleolar RNA host gene 20 (SNHG20) plays an important role in cancer. However, the function of SNHG20 in lung adenocarcinoma is unclear. The aim of our study was to investigate the roles of SNHG20 in lung adenocarcinoma. Methods: Real-time quantitative polymerasechain reaction (RT-qPCR) was used to calculate the expression of SNHG20, miR-342 and DEAD-box helicase 49 (DDX49). Dual luciferase reporter gene assay was applied to verify whether miR-342 binding to SNHG20 and DDX49. The expression correlation between miR-342 and SNHG20 or DDX49 was assessed using Pearson's correlation analysis. Results: SNHG20 and DDX49 were overexpressed, while miR-342 was lowly expressed in lung adenocarcinoma tissues and cell lines. Knockdown of SNHG20 suppressed cell proliferation, invasion and enhanced cell apoptosis. SNHG20 was found to directly bind to miR-342 and regulate the expression of miR-342. MiR-342 directly targeted DDX49 and the expression of miR-342 had negative connection with DDX49 in lung adenocarcinoma tissues. Knockdown of DDX49 inhibited the progression of lung adenocarcinoma. DDX49 partially restored the functions of SNHG20 in A549 cells. Conclusions: SNHG20 regulated lung adenocarcinoma cell proliferation, invasion and promoted cell apoptosis via miR-342/DDX49 axis. Our findings demonstrate that SNHG20/miR-342/DDX49 axis plays an important role in lung adenocarcinoma, providing a novel insight into the treatment of lung adenocarcinoma.

show abstract

MicroRNA-342-3p is a potent tumour suppressor in hepatocellular carcinoma

Cited by 131 publications

References 19 publications

Knockdown of lncRNA LINC01234 Suppresses the Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

Knockdown of lncRNA LINC01234 Suppresses the Tumorigenesis of Liver Cancer via Sponging miR-513a-5p

Baicalein Induces Apoptosis of Pancreatic Cancer Cells by Regulating the Expression of miR-139-3p and miR-196b-5p

LncRNA SNHG20 promoted proliferation, invasion and inhibited cell apoptosis of lung adenocarcinoma via sponging miR‐342 and upregulating DDX49

Contact Info

Product

Resources

About