MicroRNA-370 suppresses the retinal capillary endothelial cell growth by targeting KDR gene

Wang, X H; Chen, L

doi:10.4149/bll_2017_040

Cited by 10 publications

(14 citation statements)

References 22 publications

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“…The proliferation of HUVECs and formation of capillary‐like structures can be promoted by miR‐370 via inhibiting the expression of forkhead box 1 and maternally expressed gene 3 . But in retinal capillary endothelial cell, miR‐370 was reported to induce growth inhibition and apoptosis by reducing the expression of kinase insert domain‐containing receptor gene . Such discrepancies mechanism could be due to the contexts of different endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Profiling and functional analysis of differentially expressed circular RNAs in high glucose‐induced human umbilical vein endothelial cells

Jin

Wang

et al. 2019

FEBS Open Bio

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Dysfunction of vascular endothelial cells often results in diabetic vascular complications. Circular RNAs (circRNAs) have been implicated in the pathogenesis of various diseases, including diabetes and many vascular diseases. This study aimed to explore the roles of circRNAs in high glucose‐induced human umbilical vein endothelial cells (HUVECs) to elucidate the contributions of circRNAs to diabetic vascular complications. We subjected control and high glucose‐induced HUVECs to RNA sequencing and identified 214 differentially expressed circRNAs (versus control HUVECs, fold change ≥ 2.0, P < 0.05). We then validated seven of these differentially expressed circRNAs by qPCR ( hsa_circ_0008360 , hsa_circ_0005741 , hsa_circ_0003250 , hsa_circ_0045462 , hsa_circ_0064772 , hsa_circ_0007976 , and hsa_circ_0005263 ). A representative circRNA–microRNA (miRNA) network was constructed using the three most up‐regulated circRNAs ( hsa_circ_0008360 , hsa_circ_0000109 , and hsa_circ_0002317 ) and their putative miRNA. Bioinformatic analysis indicated that these circRNAs regulate the expressions of genes involved in vascular endothelial function and angiogenesis through targeting miRNAs. Our work highlights the potential regulatory mechanisms of three crucial circRNAs in diabetes‐associated endothelial dysfunction.

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Section: Discussionmentioning

confidence: 99%

Profiling and functional analysis of differentially expressed circular RNAs in high glucose‐induced human umbilical vein endothelial cells

Jin

Wang

et al. 2019

FEBS Open Bio

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“…MiR-370 has been experimentally validated to target ENG in ovarian cancer cells [ 75 ], yet its role in EC function and angiogenesis is unclear, as multiple reports have demonstrated conflicting evidence. Overexpression of miR-370 inhibited proliferation, migration and tube formation in human dermal microvascular ECs, retinal capillary ECs and HUVECs [ 76 , 77 ]. In contrast, miR-370 overexpression was shown to promote HUVEC proliferation, migration and tube formation to facilitate healing after finger amputation [ 78 ].…”

Section: Circulating Mir Biomarkers In Hhtmentioning

confidence: 99%

Non-Coding RNAs and Hereditary Hemorrhagic Telangiectasia

Cannavicci

Zhang

Kutryk

2020

JCM

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Non-coding RNAs (ncRNAs) are functional ribonucleic acid (RNA) species that include microRNAs (miRs), a class of short non-coding RNAs (∼21–25 nucleotides), and long non-coding RNAs (lncRNAs) consisting of more than 200 nucleotides. They regulate gene expression post-transcriptionally and are involved in a wide range of pathophysiological processes. Hereditary hemorrhagic telangiectasia (HHT) is a rare disorder inherited in an autosomal dominant fashion characterized by vascular dysplasia. Patients can develop life-threatening vascular malformations and experience severe hemorrhaging. Effective pharmacological therapies are limited. The study of ncRNAs in HHT is an emerging field with great promise. This review will explore the current literature on the involvement of ncRNAs in HHT as diagnostic and pathogenic factors.

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“…Previous studies found that miR-126, -296, and the -23-27-24 cluster exert proangiogenic effects (15)(16)(17), whereas miR-191, -92a, and -26a inhibit the angiogenic activity of ECs (18)(19)(20). miR-370-3p (miR-370), which is located in the delta-like homolog 1-iodothyronine deiodinase 3 (DIO3) region on human chromosome 14 (21), has been reported to be expressed in several types of ECs (22,23). However, the function of miR-370 in regulating the angiogenic activity of ECs still needs to be clarified.…”

mentioning

confidence: 99%

miR‐370 inhibits the angiogenic activity of endothelial cells by targeting smoothened (SMO) and bone morphogenetic protein (BMP)‐2

Becker

Zhao

et al. 2019

FASEB j.

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MicroRNAs (miRNAs) crucially modulate fundamental biologic processes such as angiogenesis. In the present study, we focused on the molecular function of miRNA‐370‐3p (miR‐370) in regulating the angiogenic activity of endothelial cells (ECs). Transfection with miR‐370 mimic (miR‐370m) significantly inhibited the sprouting of human dermal microvascular EC (HDMEC) and HUVEC spheroids and mouse aortic rings, whereas miR‐370 inhibitor (miR‐370i) promoted sprout formation. Additional in vitro assays demonstrated the pleiotropic inhibitory effects of miR‐370m on HDMEC proliferation, migration, and tube formation. Moreover, Matrigel plugs containing miR‐370m‐transfected HDMECs exhibited a reduced microvessel density after implantation into CD1 nude mice when compared with controls. In contrast, miR‐370i exerted proangiogenic effects. Mechanistic analyses revealed that miR‐370 directly targets smoothened (SMO) and down‐regulates bone morphogenetic protein (BMP)‐2 expression in HDMECs. Accordingly, inhibition of SMO by cyclopamine reversed miR‐370i‐induced HDMEC proliferation and migration. In addition, BMP‐2 treatment counteracted miR‐370m‐suppressed tube formation of HDMECs, whereas blockade of BMP‐2 with neutralizing antibody significantly inhibited miR‐370i‐induced tube formation. Taken together, these novel findings indicate that miR‐370 is a potent inhibitor of angiogenesis, which directly targets SMO and BMP‐2.—Gu, Y., Becker, V., Zhao, Y., Menger, M. D., Laschke, M. W. miR‐370 inhibits the angiogenic activity of endothelial cells by targeting smoothened (SMO) and bone morphogenetic protein (BMP)‐2. FASEB J. 33, 7213–7224 (2019). http://www.fasebj.org

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MicroRNA-370 suppresses the retinal capillary endothelial cell growth by targeting KDR gene

Abstract: MicroRNA-370 inhibits the expression of KDR gene resulting in retinal capillary endothelial cell growth inhibition and apoptosis, which could be of value in the treatment of retinal neovascularization (Tab. 1, Fig. 5, Ref. 27).

Cited by 10 publications

References 22 publications

Profiling and functional analysis of differentially expressed circular RNAs in high glucose‐induced human umbilical vein endothelial cells

Profiling and functional analysis of differentially expressed circular RNAs in high glucose‐induced human umbilical vein endothelial cells

Non-Coding RNAs and Hereditary Hemorrhagic Telangiectasia

miR‐370 inhibits the angiogenic activity of endothelial cells by targeting smoothened (SMO) and bone morphogenetic protein (BMP)‐2

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