MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Wu, Rimao; Li, Hu; Zhai, Lili; Zou, Xiaoting; Meng, Jiao; Zhong, Ran; Li, Chang-Yin; Wang, Haixia; Zhang, Yong; Zhu, Dahai

doi:10.1038/ncomms8713

Cited by 63 publications

(67 citation statements)

References 54 publications

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“…miRs-1, -133, and -206 are up-regulated during myogenic differentiation and can be inhibited by inhibitors of the PI3K/AKT pathway [25]. In addition, miR-431 was found to enhance muscle generation by targeting Pax7 in satellite cells primed for differentiation during muscle regeneration [26].…”

Section: Introductionmentioning

confidence: 99%

“…Frozen tissue sections (10-μm thick) were fixed in 4% paraformaldehyde and stained with antibody to laminin (Sigma), Pax7 (Developmental Studies Hybridoma Bank, Iowa City, USA), or MyoD (Santa Cruz Biotechnology, Santa Cruz, USA), as described previously [26]. Cross-sectional areas (CSAs) were measured using NIH Image J software (http://rsb.info.nih.gov/ij).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

Zeng

Han

et al. 2016

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Skeletal muscle mass and homeostasis during postnatal muscle development and regeneration largely depend on adult muscle stem cells (satellite cells). We recently showed that global overexpression of miR-378 significantly reduced skeletal muscle mass in mice. In the current study, we used miR-378 transgenic (Tg) mice to assess the in vivo functional effects of miR-378 on skeletal muscle growth and regeneration. Cross-sectional analysis of skeletal muscle tissues showed that the number and size of myofibers were significantly lower in miR-378 Tg mice than in wild-type mice. Attenuated cardiotoxin-induced muscle regeneration in miR-378 Tg mice was found to be associated with delayed satellite cell activation and differentiation. Mechanistically, miR-378 was found to directly target Igf1r in muscle cells both in vitro and in vivo. These miR-378 Tg mice may provide a model for investigating the physiological and pathological roles of skeletal muscle in muscle-associated diseases in humans, particularly in sarcopenia.

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Section: Introductionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

Zeng

Han

et al. 2016

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“…More recently, it has been proposed that specification occurs during gastrulation and is initiated by neural plate border specifier genes such as Pax7 [129•,130]. Originally identified as being required for muscle cell differentiation, homeostasis, and repair [131], Pax7 is targeted directly by miR-431 in muscle satellite cells, the overexpression of which increases myogenic differentiation [132]. Thus, miss-expression of miR-431 could negatively impact NCC development.…”

Section: Micrornas Involved In Neural Crest Induction and Differentiamentioning

confidence: 99%

microRNA Regulation of Skeletal Development

Sera

Nieden

2017

Curr Osteoporos Rep

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Purpose of review Osteogenesis is a complex process involving the specification of multiple progenitor cells and their maturation and differentiation into matrix-secreting osteoblasts. Osteogenesis occurs not only during embryogenesis, but also during growth, after an injury, and in normal homeostatic maintenance. While much is known about osteogenesis associated regulatory genes, the role of microRNAs, which are epigenetic regulators of protein expression, is just beginning to be explored. While miRNAs do not abrogate all protein expression, their purpose is to finely tune it, allowing for a timely and temporary protein down-regulation. Recent findings The last decade has unveiled a multitude of microRNAs that regulate key proteins within the osteogenic lineage, thus qualifying them as ‘osteomiRs’. These miRNAs may endogenously target an activator or inhibitor of differentiation, and depending on the target, may either lead to the prolongation of a progenitor maintenance state or to early differentiation. Interestingly, cellular identity seems intimately coupled to the expression of miRNAs, which participate in the suppression of previous and subsequent differentiation steps. In such cases where key osteogenic proteins were identified as direct targets of miRNAs in non-bone cell types, or through bioinformatic prediction, future research illuminating the activity of these miRNAs during osteogenesis will be extremely valuable. Summary Many bone related diseases involve the dysregulation of transcription factors or other proteins found within osteoblasts and their progenitors, and the dysregulation of miRNAs, which target such factors, may play a pivotal role in disease etiology, or even as a possible therapy.

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“…Interestingly enough, miR-431 attenuates the muscular dystrophic phenotype in mdx mice (a model of Duchenne muscular dystrophy) and has been proposed as a potential therapeutic target in muscular diseases [68]. In addition, miR-431 is a key post-transcriptional regulator for axon regeneration, during neural development, for brain function, and in neurological diseases [69], although this aspect has not yet been explored in relation to MT.…”

Section: Effects Of Mt In Muscle Regenerationmentioning

confidence: 99%

Unraveling molecular determinants of manual therapy: an approach to integrative therapeutics for the treatment of fibromyalgia and CFS/ME

Espejo¹,

García-Escudero²,

Oltra³

2018

Preprint

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Abstract:Application of protocols without parameter standardization and rigorous controls has led manual therapy (MT) and other physiotherapy approaches to controversial outcomes. Thus, there is an urgency to carefully define standard protocols that elevate physiotherapy treatments to rigorous scientific demands. One way this can be achieved is by studying gene expression and additional physiological changes that associate to particular, parameter-controlled, treatments in animal models and translating this knowledge to properly design objective, quantitatively-monitored clinical trials. Here, we propose a Molecular Physiotherapy Approach (MPTA), requiring multidisciplinary teams, to uncover the scientific reasons behind the numerous reports of MT that historically attribute benefits to these treatments. The review focuses in the identification of MT-induced physiological and molecular responses that could be used for the treatment of fibromyalgia (FM) and CFS/ME. The systemic effect associated to mechanical-load responses is considered of particular relevance as it suggests that defined, low-pain areas could be selected for treatments with overall benefits, an aspect that might result essential to treat FM. Additionally, MT can provide muscle conditioning to sedentary patients without demanding strenuous physical effort, detrimental for CFS/ME patients, placing MT as a real option for integrative medicine programs to treat FM and CFS/ME.

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MicroRNA-431 accelerates muscle regeneration and ameliorates muscular dystrophy by targeting Pax7 in mice

Cited by 63 publications

References 54 publications

miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

microRNA Regulation of Skeletal Development

Unraveling molecular determinants of manual therapy: an approach to integrative therapeutics for the treatment of fibromyalgia and CFS/ME

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