MicroRNA-4316 inhibits gastric cancer proliferation and migration via directly targeting VEGF-A

Abstract

Background and aims: microRNAs (miRNAs) have been reported to regulate proliferation and migration by downregulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its clinical significance in gastric cancer (GC). Methods: The clinical tissues of the GC patients for miR-4316 and VEGF-A were detected by qRT-PCR. The protein levels of VEGF-A and c-Met were determined by western blotting. Cell Proliferation, migration, and colony forming assays were conducted to show whether miR-4316 affects proliferation by CCK-8, migration by transwell, wound healing and colony formation assays. The bioinformatic methods and luciferase reporter assay were applied to detect the relationship between miRNA and VEGF-A on its targeting 3-untranslated regions (3-UTRs). CCK-8, colony formation, wound healing, and transwell assay were performed to explore the function of miR-4316. Results: The results of qRT-PCR indicated that miR-4316 expression level was significantly downregulated in human GC tissues and GC cell lines compared with their control. miR-4316 inhibited proliferation, migration and colony formation in GC cell lines by reducing VEGF-A. And western blot results indicated that miR-4316 significantly inhibited GC through repressing VEGF-A and c-Met. The investigation of Luciferase assay indicated that VEGF-A is a direct target gene of miR-4316. Conclusions: miR-4316 suppressed proliferation and migration of GC through the VEGF-A gene. MiR-4316 acts as a tumor suppressor by targeting VEGF-A and this indicated that MiR-4316 might be a potential therapeutic target for GC.

Characterization of a Novel LUCAT1/miR-4316/VEGF-A Axis in Metastasis and Glycolysis of Lung Adenocarcinoma

“…The classic mechanism for miRNA was modulate target genes by binding on its complementary binding sites of mRNA 3′-UTR, inhibiting target mRNA translating or promoting mRNA degradation. In this study, we confirmed that VEGFA may serve as a functional target of miR-4316, which has been proved to have inhibitory effects on proliferation and migration (Mousa et al, 2020). Therefore, we suspected that VEGFA is one of the miR-4316 target genes in LUAD cells.…”

Downregulation of microRNA‑605 indicates poor prognosis and promotes the progression of osteosarcoma

“…With the development of molecular biology, an increasing number of biomarkers, such as miRNAs, that play roles in the progression of cancers and tumors have been identified, and the therapy of different cancers, including OS, has improved rapidly (23)(24)(25). miRNAs have been reported to play vital biomarker roles in various cancers, such as lung cancer, ovarian cancer, breast cancer, and gastric cancer (11,(26)(27)(28)(29).…”

Recent advances of miRNAs in the development and clinical application of gastric cancer

“…Knockdown of RASSF8 promoted the transcriptional activity of NF-κB and p65 translocation. Mousa et al [ 66 ] showed that miR-4316 inhibited vascular endothelial growth factor A (VEGF-A) expression and induced the suppression of migration and colony formation in tumor cell lines. β-Elemene extracted from Chinese herbal medicine showed a good effect on the inhibition of metastasis in nude mice.…”