MicroRNA-451 induces epithelial–mesenchymal transition in docetaxel-resistant lung adenocarcinoma cells by targeting proto-oncogene c-Myc

Chen, Dongqin; Huang, Jiayuan; Zhang, Kai; Pan, Banzhou; Chen, Jing; De, Wei; Wang, Rui; Chen, Longbang

doi:10.1016/j.ejca.2014.09.008

Cited by 71 publications

(75 citation statements)

References 47 publications

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“…Chemotherapeutic agents are limited by acquired resistance, which has undoubtedly become a stumbling block to the successful treatment and management of lung cancer [29]. To date, methods to resist drug resistance and improve clinical outcomes have not been developed, despite extensive studies.…”

Section: Discussionmentioning

confidence: 99%

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Profiles and Bioinformatics Analysis of Differentially Expressed Circrnas in Taxol-Resistant Non-Small Cell Lung Cancer Cells

Chen

Liu

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) act as microRNA (miRNA) sponges that regulate gene expression and are involved in physiological and pathological processes. In this study, we evaluated the roles of circRNAs in the chemoresistance of non-small cell lung cancer (NSCLC) to taxol. Methods: High-throughput circRNA microarrays were employed to investigate the circRNA profiles of parental A549 and taxol-resistant A549/Taxol cells. We predicted the miRNA targets of differentially expressed circRNAs via miRNA prediction software and then constructed a circRNA/miRNA network using Cytoscape. Bioinformatics analyses were performed to annotate dysregulated circRNAs in detail. Results: We detected 2909 significantly upregulated and 8372 downregulated circRNAs in A549/Taxol cells compared with A549 cells. The circRNA/miRNA network displayed their interactions, suggesting that circRNAs exert biological effects by absorbing and sequestering miRNA molecules. Computational Gene Ontology and pathway analyses were used to determine the biological function and signaling pathways of host genes of dysregulated circRNAs and to identify potential molecular mechanisms prompting the resistance of NSCLC to taxol. Conclusion: This study focusing on circRNAs related to taxol resistance provides a basis for clarifying the development and progression of drug resistance and for identifying therapeutic targets in NSCLC.

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Section: Discussionmentioning

confidence: 99%

“…A number of pathological and molecular mechanisms related to chemoresistance have been evaluated. miRNAs and lncRNAs are often involved in controlling these complex processes in lung cancer [29,30,[44][45][46]. Recent research has focused on the contribution of circRNAs to gene regulation.…”

Section: Discussionmentioning

confidence: 99%

Profiles and Bioinformatics Analysis of Differentially Expressed Circrnas in Taxol-Resistant Non-Small Cell Lung Cancer Cells

Chen

Liu

et al. 2018

Cell Physiol Biochem

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“…The expression level of miR-301a were quantified by using miRNA-specific TaqMan MiRNA Assay Kit (Applied Biosystems) and expression of RNU6B (Qiagen, USA) was used as endogenous control [22]. For determining the expression of PTEN mRNA, total RNA was reverse transcribed to cDNA and qRT-PCR was performed as described previously and expression of GAPDH was used as endogenous control.…”

Section: Quantitative Real-time Pcr (Qrt-pcr) Assaymentioning

confidence: 99%

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Cui

Li²,

Lv³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Although microRNA-301a has been reported to function as an oncogene in many human cancers, the roles of miR-301a in malignant melanoma (MM) is unclear. The present study aims to investigate the functional roles of miR-301a in MM and its possible molecular mechanisms. Methods: Quantitative real-time PCR (qRT-PCR) assay was performed to detect the expression of miR-301a in MM tissues, and analyze its correlation with metastasis and prognosis of MM patients. In vitro, miR-301a was ectopically expressed using overexpression and knock-down strategies, and the effects of miR-301a expression on growth, apoptosis, migration, invasion and chemosensitivity of MM cells were further investigated. Furthermore, the potential and functional target gene was identified by luciferase reporter, qRT-PCR, Western blot assays. Results: We showed that the expression of miR-301a was significantly upregulated in MM tissues, and upregulation of miR-301a correlated with metastasis and poor prognosis of MM patients. Transfection of miR-301a/inhibitor significantly inhibited growth, colony formation, migration, invasion and enhanced apoptosis and chemosensitivity in MM cells, while transfection of miR-301a/mimic could induce the inverse effects on phenotypes of MM cells. Luciferase reporter, qRT-PCR and Western blot assays showed that phosphatase and tensin homolog (PTEN) was a direct and functional target of miR-301a. It was also observed that the Akt and FAK signaling pathways were involved in miR-301/PTEN-promoting MM progression. Conclusion: Taken together, our study suggests that miR-301a may be used as a potential therapeutic target in the treatment of human MM.

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“…Our data show that inhibition of Myc prevents activated T cells to secret IL-2, suggesting that there may be a regulatory loop between IL-2 and Myc. It has been reported that miR-451a regulates the growth of tumors such as acute lymphoblast leukemia, head and neck squamous cell carcinomas, and lung adenocarcinoma by directly targeting Myc (35)(36)(37). Here, we show that miR451a directly targets Myc in T cells in vitro, and their expressions are negatively correlated in CD4 ϩ T cells of DCM patients in vivo, probably suggesting a role of miR-451-Myc pathway in CD4 ϩ T cell abnormality in DCM conditions.…”

Section: Discussionmentioning

confidence: 99%

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

Zeng

Wang

et al. 2017

Journal of Biological Chemistry

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MicroRNA-451 induces epithelial–mesenchymal transition in docetaxel-resistant lung adenocarcinoma cells by targeting proto-oncogene c-Myc

Cited by 71 publications

References 47 publications

Profiles and Bioinformatics Analysis of Differentially Expressed Circrnas in Taxol-Resistant Non-Small Cell Lung Cancer Cells

Profiles and Bioinformatics Analysis of Differentially Expressed Circrnas in Taxol-Resistant Non-Small Cell Lung Cancer Cells

Expression of MicroRNA-301a and its Functional Roles in Malignant Melanoma

Down-regulation of microRNA-451a facilitates the activation and proliferation of CD4+ T cells by targeting Myc in patients with dilated cardiomyopathy

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