MicroRNA-454-3p inhibits cervical cancer cell invasion and migration by targeting c-Met

Guo, Yan; Tao, Min; Jiang, Min

doi:10.3892/etm.2018.5714

Cited by 12 publications

(13 citation statements)

References 19 publications

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“…MMP9 activity is reduced by miR-34a-5p, miR-34b-3p, miR-34c-5p, and miR-34c-3p. In SiHa and HeLa cells, MMP2 and MMP9 activity reduction by miR-183, miR-125a, miR-491-5p, and miR-454-3p was reported [72,73,74,75]; nevertheless, for miR-34 family members in cervical cell lines, there are no data. However, MMP2 and MMP9 are inhibited by miR-34a-5p in esophageal squamous cell carcinoma [48].…”

Section: Discussionmentioning

confidence: 99%

“…However, MMP2 and MMP9 are inhibited by miR-34a-5p in esophageal squamous cell carcinoma [48]. The differential effect between cervical cancer cell lines and esophageal squamous cell lines could be a reflection of the cellular context of the gene environment, highlighting that MMP2 and MMP9 are downregulated by several miRNAs, independent of UTR binding sites [73,74,75]. Additionally, MMP2 and MMP9 could be regulated indirectly via their activators (MT1-MMP and heregulin-B1, plasmin, U-Plasminogen Activator (uPA), MMP-3, MMP-2, and MMP-13) [76,77,78,79] and/or inhibitors (Tissue Inhibitor of Metalloproteinase (TIM-2 and TIM-1)) [79,80].…”

Section: Discussionmentioning

confidence: 99%

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5p and 3p Strands of miR-34 Family Members Have Differential Effects in Cell Proliferation, Migration, and Invasion in Cervical Cancer Cells

Córdova-Rivas

Fraire-Soto

Torres

et al. 2019

IJMS

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The micro RNA (miR)-34 family is composed of 5p and 3p strands of miR-34a, miR-34b, and miR-34c. The 5p strand’s expression and function is studied in cervical cancer. The 3p strand’s function and regulation remain to be elucidated. To study the function of the passenger strands of miR-34 family members, we overexpressed 5p and 3p strands using a synthetic miRNA in cervical cell lines. Cell proliferation was evaluated using crystal violet. Migration and invasion were tested using transwell assays, Western blot, and zymography. Possible specific targets and cell signaling were investigated for each strand. We found that miR-34a-5p inhibited proliferation, migration, and cell invasion accompanied by matrix metalloproteinase 9 (MMP9) activity and microtubule-associated protein 2 (MAP2) protein reduction. We also found that miR-34b-5p and miR-34c-5p inhibit proliferation and migration, but not invasion. In contrast, miR-34c-5p inhibits MMP9 activity and MAP2 protein, while miR-34b-5p has no effect on these genes. Furthermore, miR-34a-3p and miR-34b-3p inhibit proliferation and migration, but not invasion, despite the later reducing MMP2 activity, while miR-34c-3p inhibit proliferation, migration, and cell invasion accompanied by MMP9 activity and MAP2 protein inhibition. The difference in cellular processes, MMP2 and MMP9 activity, and MAP2 protein inhibition by miR-34 family members suggests the participation of other regulated genes. This study provides insights into the roles of passenger strands (strand*) of the miR-34 family in cervical cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

5p and 3p Strands of miR-34 Family Members Have Differential Effects in Cell Proliferation, Migration, and Invasion in Cervical Cancer Cells

Córdova-Rivas

Fraire-Soto

Torres

et al. 2019

IJMS

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“…Additionally, the protective roles of miR-132 [ 59 ] and miR-183 [ 60 ] in the invasion of CC are related to the decreased production of MMP2 and/or MMP9. miR-454-3p restrained CC cell migration and invasion partly due to targetting c-Met, correspondingly leading to the down-regulation of p-AKT, MMP-2, and MMP-9, the downstream proteins of c-Met [ 61 ]. miR-486-3p is a significantly down-regulated miRNA in CC and functions to repress CC cell metastasis via down-regulating ECM1 [ 62 ].…”

Section: Oncosuppressor Mirnas In the Metastasis Of CCmentioning

confidence: 99%

The role of miRNAs in the invasion and metastasis of cervical cancer

Wang

Chen

2019

Bioscience Reports

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Cervical cancer (CC) with early metastasis of the primary tumor results in poor prognosis and poor therapeutic outcomes. MicroRNAs (miRNAs) are small, noncoding RNA molecules that play a substantial role in regulating gene expression post-transcriptionally and influence the development and progression of tumors. Numerous studies have discovered that miRNAs play significant roles in the invasion and metastasis of CC by affecting specific pathways, including Notch, Wnt/β-catenin, and phosphoinositide-3 kinase (PI3K)-Akt pathways. miRNAs also effectively modulate the process of epithelial–mesenchymal transition. Many studies provide new insights into the role of miRNAs and the pathogenesis of metastatic CC. In this review, we will offer an overview and update of our present understanding of the potential roles of miRNAs in metastatic CC.

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“…On the other hand, the highest level of c-Met mRNA found in C33A cells may be due to the fact that the muted form of p53 in these cells does not inhibit the expression of c-Met. The level of c-Met transcript may be partially regulated by miR-23a-3p, miR-23c, miR-130a-5p (predicted by TargetScan), miR-34a 38 , miR-138 39 , miR-454-3p 40 , and miR-23b-3p. The difference in the expression of c-Met among C33A, www.nature.com/scientificreports www.nature.com/scientificreports/ CaSki and HaCaT could be explained by the abundance and activity of different miRNAs and other regulatory mechanisms of gene expression.…”

Section: Discussionmentioning

confidence: 99%

MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression

Campos-Viguri

Peralta‐Zaragoza

Jiménez‐Wences

et al. 2020

Sci Rep

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Malignant transformation and progression in cancer is associated with the altered expression of multiple miRNAs, which are considered as post-transcriptional regulators of genes participating in various cellular processes. Although, it has been proposed that miR-23b-3p acts as a tumor suppressor in cervical cancer (CC), not all the pathways through which it alters the cellular processes have been described. The present study examines whether miR-23b-3p directly represses the c-Met expression and that consequently modifies the proliferation, migration and invasion of C33A and CaSki cells. c-Met has five microRNA response elements (MREs) for miR-23b-3p in the 3′-UTR region. The ectopic overexpression of miR-23b-3p significantly reduces c-Met expression in C33A and CaSki cells. The overexpression of miR-23b-3p reduces proliferation, migration and invasion of CaSki cells and the proliferation and invasion in C33A cells. In CaSki cells, the activation of Gab1 and Fak, downstream of c-Met, is reduced in response to the overexpression of miR-23b-3p. Together, the results in the present study indicate that miR-23b-3p is a tumor suppressor that modulates the progression of CC via posttranscriptional regulation of the c-Met oncogene. Cervical cancer (CC) is the fourth most common malignancy in women around the world, with 569,847 newly diagnosed cases and 311,365 deaths registered in 2018 1. The late detection of CC increases the risk of metastasis and death, with a third of CC patients diagnosed in the advanced stages of the disease 2. This has driven the search for progression and prognosis markers which enable the improvement of treatment scheme selection. While persistent high-risk human papillomavirus (HR HPV) infection plays a central role in the genesis of CC 3 , other factors also contribute to the origin and progression of this malignancy. Some mutations, modifications in specific and global DNA methylation, changes to the expression profiles of chromatin-modifying enzymes and the aberrant expression of microRNAs (miRNAs) are events frequently detected in CC 4,5. Regulating gene

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MicroRNA-454-3p inhibits cervical cancer cell invasion and migration by targeting c-Met

Cited by 12 publications

References 19 publications

5p and 3p Strands of miR-34 Family Members Have Differential Effects in Cell Proliferation, Migration, and Invasion in Cervical Cancer Cells

5p and 3p Strands of miR-34 Family Members Have Differential Effects in Cell Proliferation, Migration, and Invasion in Cervical Cancer Cells

The role of miRNAs in the invasion and metastasis of cervical cancer

MiR-23b-3p reduces the proliferation, migration and invasion of cervical cancer cell lines via the reduction of c-Met expression

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