MicroRNA-466 and microRNA-200 increase endothelial permeability in hyperglycemia by targeting Claudin-5

Kujawa, Marisa; O’Meara, Megan M.; Li, Hainan; Xu, Liping; Venkata, Sai Pranathi Meda; Nguyen, Huong Thanh; Minjares, Morgan; Zhang, Kezhong; Wang, Jiemei

doi:10.1016/j.omtn.2022.07.002

Cited by 12 publications

(6 citation statements)

References 46 publications

(59 reference statements)

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“…Moreover, for the first time, we observed that the inhibition of miR‐200a/b/c‐3p moderated HG‐induced cell proliferation and DNA damage in HFF‐1 cells and that miR‐200a/b/c‐3p inhibitors could promote wound healing in DFU mice. The observation was in line with their role in the proliferation of HG‐treated endothelial cells and wound closure of the type 2 diabetes mouse model 29 . Collectively, our findings established the function of miR‐200 family members in enhancing the inhibition of cell proliferation, DNA damage, and wound healing delays of DFU in vitro or in vivo models.…”

Section: Discussionsupporting

confidence: 81%

GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR‐200/ataxia telangiectasia mutated axis in diabetic wound healing

Liang,

Lin,

Qiu

et al. 2024

The Kaohsiung J of Med Scie

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Diabetic foot ulcer (DFU) is a serious complication of diabetic patients which negatively affects their foot health. This study aimed to estimate the role and mechanism of the miR‐200 family in DNA damage of diabetic wound healing. Human foreskin fibroblasts (HFF‐1 cells) were stimulated with high glucose (HG). Db/db mice were utilized to conduct the DFU in vivo model. Cell viability was evaluated using 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2‐H‐tetrazolium bromide assays. Superoxide dismutase activity was determined using detection kits. Reactive oxygen species determination was conducted via dichlorodihydrofluorescein‐diacetate assays. Enzyme‐linked immunosorbent assay was used to evaluate 8‐oxo‐7,8‐dihydro‐2′deoxyguanosine levels. Genes and protein expression were analyzed by quantitative real‐time polymerase chain reaction, western blotting, or immunohistochemical analyses. Luciferase reporter gene and RNA immunoprecipitation assays determined the interaction with miR‐200a/b/c‐3p and GLI family zinc finger protein 2 (GLI2) or ataxia telangiectasia mutated (ATM) kinase. HG repressed cell proliferation and DNA damage repair, promoted miR‐200a/b/c‐3p expression, and suppressed ATM and GLI2. MiR‐200a/b/c‐3p inhibition ameliorated HG‐induced cell proliferation and DNA damage repair repression. MiR‐200a/b/c‐3p targeted ATM. Then, the silenced ATM reversed the miR‐200a/b/c‐3p inhibition‐mediated alleviative effects under HG. Next, GLI2 overexpression alleviated the HG‐induced cell proliferation and DNA damage repair inhibition via miR‐200a/b/c‐3p. MiR‐200a/b/c‐3p inhibition significantly promoted DNA damage repair and wound healing in DFU mice. GLI2 promoted cell proliferation and DNA damage repair by regulating the miR‐200/ATM axis to enhance diabetic wound healing in DFU.

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Section: Discussionsupporting

confidence: 81%

GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR‐200/ataxia telangiectasia mutated axis in diabetic wound healing

Liang,

Lin,

Qiu

et al. 2024

The Kaohsiung J of Med Scie

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“…The mechanism of this competition is also unknown but the binding site of PUMs and miRNA do not need to be in close proximity. miRNA-200 and − 466 were recently reported as a miRNA’s that can directly bind near to the rs10314 site in human CLDN-5 mRNA [ 30 ] but neither miRNAs are expressed in HEK-293 cells [ 22 ].…”

Section: Discussionmentioning

confidence: 99%

Pumilio-1 mediated translational control of claudin-5 at the blood-brain barrier

Hashimoto,

Greene,

Hanley

et al. 2024

Fluids Barriers CNS

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Claudin-5 is one of the most essential tight junction proteins at the blood-brain barrier. A single nucleotide polymorphism rs10314 is located in the 3’-untranslated region of claudin-5 and has been shown to be a risk factor for schizophrenia. Here, we show that the pumilio RNA-binding protein, pumilio-1, is responsible for rs10314-mediated claudin-5 regulation. The RNA sequence surrounding rs10314 is highly homologous to the canonical pumilio-binding sequence and claudin-5 mRNA with rs10314 produces 25% less protein due to its inability to bind to pumilio-1. Pumilio-1 formed cytosolic granules under stress conditions and claudin-5 mRNA appeared to preferentially accumulate in these granules. Added to this, we observed granular pumilio-1 in endothelial cells in human brain tissues from patients with psychiatric disorders or epilepsy with increased/accumulated claudin-5 mRNA levels, suggesting translational claudin-5 suppression may occur in a brain-region specific manner. These findings identify a key regulator of claudin-5 translational processing and how its dysregulation may be associated with neurological and neuropsychiatric disorders. Graphical Abstract

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“…Persistent hyperglycemia also results in increased vascular or lymphatic permeability. Several mechanisms, including transcriptional suppression of claudin-5 ( 39 ), activating canonical and rapid non-canonical NOTCH1 pathways in endothelial cells ( 40 ), and impaired activin receptor-like kinase 1 signaling ( 41 ) have been reported to be involved in this process. Furthermore, hyperglycemia was reported to accelerate pancreatic cancer metastasis via aggravating the hypoxia microenvironment by induction of hypoxia inducible factor 1 ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a nomogram to predict occult cervical metastasis in early oral squamous cell carcinoma

Xu¹,

Chen²,

Yin³

et al. 2023

Ann Transl Med

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Background: Lack of adequate objectivity and universality, available models are still difficult to be applied to clinical practice in predicting occult cervical metastasis of early oral squamous cell carcinoma (OSCC).Taking abnormal metabolic state into consideration, the current model is helpful to distinguish those patients with or without occult cervical metastasis.Methods: This study retrospectively analyzed 330 OSCC patients initially diagnosed cT1-2N0M0 stage and received neck dissection from January 2020 to July 2022. The occult cervical metastasis was identified by pathological examination.. After screening independent risk factors using logistic regression, patients were divided into training and validation cohorts at the ratio of 2:1 randomly, and a novel diagnostic model was constructed. Performances of this model were evaluated by the area under the curve (AUC), calibrating curve, decision curve analysis (DCA) and clinical impact curve (CIC).Results: Of the 330 included patients {age mean [standard deviation (SD)], 61.24 (12.99) years; 202 (61.2%) males}, 49 (14.8%) had occult nodal metastasis. Five variables, including body mass index (BMI) [high odds

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MicroRNA-466 and microRNA-200 increase endothelial permeability in hyperglycemia by targeting Claudin-5

Cited by 12 publications

References 46 publications

GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR‐200/ataxia telangiectasia mutated axis in diabetic wound healing

GLI family zinc finger protein 2 promotes skin fibroblast proliferation and DNA damage repair by targeting the miR‐200/ataxia telangiectasia mutated axis in diabetic wound healing

Pumilio-1 mediated translational control of claudin-5 at the blood-brain barrier

Development and validation of a nomogram to predict occult cervical metastasis in early oral squamous cell carcinoma

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