MicroRNA-488-3p Regulates Neuronal Cell Death in Cerebral Ischemic Stroke Through Vacuolar Protein Sorting 4B (VPS4B)

Zhou, Li; Yang, Wanxin; Yao, En‐Ping; Li, Haiyan; Wang, Jihui; Wang, Kun; Zhong, Xiaohua; Peng, Zhongxing; Huang, Xuming

doi:10.2147/ndt.s255666

Cited by 11 publications

(3 citation statements)

References 38 publications

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“… 57 The up-regulated expression of hsa-miR-488-3p inhibited the death of neuron cells and the ischemic cerebral infarction in IS mice. 58 Hsa-miR-488-3p is also involved in regulating the activation of astrocytes in IS. 59 In this study, we also found that hsa-miR-3150b-3p, hsa-miR-541-5p, hsa-miR-184 and hsa-miR-488-3p were abnormally expressed in IS.…”

Section: Discussionmentioning

confidence: 99%

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Zhang¹,

Han²,

Xu³

et al. 2023

IJGM

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Background:The specific molecular mechanistic link between atherosclerotic plaques and ischemic stroke (IS) is not clear. The aim of this study is to explore the potential molecular relationship between atherosclerotic plaques and IS. Methods: All data were downloaded from the Gene Expression Omnibus (GEO) database. Key hub differentially expressed mRNAs (DEmRNAs) related to atherosclerotic plaques and IS were identified by differential expression analysis and least absolute shrinkage and selection operator (LASSO) analysis. Subsequently, a diagnostic model was established based on the expression of key hub DEmRNAs and logistic regression. In order to understand the molecular mechanism of key hub DEmRNAs, the transcription factor (TF) regulatory network and mRNA-miRNA-lncRNA regulatory network were also constructed. In addition, functional enrichment analysis and single-sample Gene Set Enrichment Analysis (ssGSEA) analysis were also performed. Results: Four key hub DEmRNAs (ADCY3, CLDN7, PPM1B and RRAS2) were identified by differential expression analysis and LASSO analysis. Moreover, the diagnostic model based on four key hub DEmRNAs has excellent diagnostic accuracy. We also found that Type 1 T helper cell may be associated with IS caused by atherosclerosis based on ssGSEA analysis. In the mRNA-miRNA-lncRNA regulatory network, we found that multiple signaling axes such as RRAS2-hsa-miR-3150b-3p-ILF3-AS1, PPM1B-hsa-miR -541-5p-LINC00294, CLDN7-hsa-miR-184-LINC00467 and ADCY3-hsa-miR-488-3p-URB1-AS1 may play an important role in the progression of IS. In addition, some signaling pathways, including chemokine signaling pathway, MAPK signaling pathway and cAMP signaling pathway, may be involved in regulating IS. Conclusion:The identified key molecules, signaling pathways and immune cells may help to provide a theoretical basis for exploring the relationship between atherosclerotic plaque and the progression of IS.

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Section: Discussionmentioning

confidence: 99%

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Zhang¹,

Han²,

Xu³

et al. 2023

IJGM

View full text Add to dashboard Cite

show abstract

“…miR-488 is usually (but not always) downregulated in cancer, acute myocardial infarction, and other conditions [ 28 ]. In the brain, miR-488 plays an anti-apoptotic role and can limit neuronal apoptosis in epilepsy or ischemic stroke [ 48 , 49 ]. In the HSCR colon, however, miR-488 is upregulated, where it is suggested to target DCX to limit cell migration and proliferation [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System

Pai,

Sengupta,

Heuckeroth

2023

ncRNA

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The enteric nervous system (ENS) is an essential network of neurons and glia in the bowel wall. Defects in ENS development can result in Hirschsprung disease (HSCR), a life-threatening condition characterized by severe constipation, abdominal distention, bilious vomiting, and failure to thrive. A growing body of literature connects HSCR to alterations in miRNA expression, but there are limited data on the normal miRNA landscape in the developing ENS. We sequenced small RNAs (smRNA-seq) and messenger RNAs (mRNA-seq) from ENS precursor cells of mid-gestation Ednrb-EGFP mice and compared them to aggregated RNA from all other cells in the developing bowel. Our smRNA-seq results identified 73 miRNAs that were significantly enriched and highly expressed in the developing ENS, with miR-9, miR-27b, miR-124, miR-137, and miR-488 as our top 5 miRNAs that are conserved in humans. However, contrary to prior reports, our follow-up analyses of miR-137 showed that loss of Mir137 in Nestin-cre, Wnt1-cre, Sox10-cre, or Baf53b-cre lineage cells had no effect on mouse survival or ENS development. Our data provide important context for future studies of miRNAs in HSCR and other ENS diseases and highlight open questions about facility-specific factors in development.

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“…Most studies from various researchers have demonstrated unidirectional effectiveness regarding the neuroprotective role of miR-10b-3p [ 116 , 117 ], miR-124-3p [ 119 , 120 ], miR-141-3p [ 107 , 131 ], miR-153-3p [ 141 , 142 ], miR-182-5p [ 101 , 150 ], miR-20a-3p [ 162 , 163 ], miR-22-3p [ 171 , 172 ], miR-24-3p [ 175 , 176 ], miR-27a-3p [ 181 , 182 , 183 ], miR-342-5p [ 191 , 192 ], miR-455-3p [ 200 , 201 ], miR-488-3p [ 203 , 204 ], and miR-92b-3p [ 100 , 221 ] as well as the damaging role of miR-141-3p [ 107 , 131 ], miR-155-5p [ 111 , 112 ], and miR-182 [ 148 , 149 ]. Therefore, these specific microRNAs should be considered as priority targets for further translation into clinical practice.…”

Section: Regulation Of Signaling Pathways In Ischemia and Reperfusion...mentioning

confidence: 99%

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

Tregub,

Ibrahimli,

Averchuk

et al. 2023

IJMS

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In recent times, there has been a significant increase in researchers’ interest in the functions of microRNAs and the role of these molecules in the pathogenesis of many multifactorial diseases. This is related to the diagnostic and prognostic potential of microRNA expression levels as well as the prospects of using it in personalized targeted therapy. This review of the literature analyzes existing scientific data on the involvement of microRNAs in the molecular and cellular mechanisms underlying the development of pathologies such as Alzheimer’s disease, cerebral ischemia and reperfusion injury, and dysfunction of the blood–brain barrier.

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MicroRNA-488-3p Regulates Neuronal Cell Death in Cerebral Ischemic Stroke Through Vacuolar Protein Sorting 4B (VPS4B)

Cited by 11 publications

References 38 publications

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Uncovering Candidate mRNAs, Signaling Pathways and Immune Cells in Atherosclerotic Plaque and Ischemic Stroke

Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System

The Role of microRNAs in Epigenetic Regulation of Signaling Pathways in Neurological Pathologies

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