MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer

Lan, Gongbin; Liu, Yang; Xie, Xubiao; Peng, Longkai; Wang, Yi

doi:10.5114/aoms.2015.52359

Cited by 39 publications

(36 citation statements)

References 31 publications

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“…For example, miR-490-5p serves as a suppressor in renal cell carcinoma with a significant decrease in the expression level and its inhibitory effect can be reversed by exogenous PIK3CA [29]. Besides, researchers also found that the expression of miR-490-5p frequently decreased in the HCC tissue samples and cells as a novel tumor suppressor by directly targeting c-FOS [31]. Meanwhile, the anti-oncogenic role of miR-490-5p has been verified for its suppressive impacts on bladder cancer carcinogenesis [31,32].…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that miR-490-5p is dramatically downregulated in several forms of human cancer including renal cancer, colorectal cancer, and bladder cancer [29,30,31]. For example, miR-490-5p serves as a suppressor in renal cell carcinoma with a significant decrease in the expression level and its inhibitory effect can be reversed by exogenous PIK3CA [29].…”

Section: Discussionmentioning

confidence: 99%

“…Besides, researchers also found that the expression of miR-490-5p frequently decreased in the HCC tissue samples and cells as a novel tumor suppressor by directly targeting c-FOS [31]. Meanwhile, the anti-oncogenic role of miR-490-5p has been verified for its suppressive impacts on bladder cancer carcinogenesis [31,32]. In this study, we quantified the expression of miR-490-5p in HCC tissue samples and HCC-derived cells.…”

Section: Discussionmentioning

confidence: 99%

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MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells

Liu

et al. 2017

Pharmacology

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Objective: To verify that miR-490-5p could influence hepatocellular carcinoma (HCC) cells' proliferation, invasion, cycle, and apoptosis by targeting BUB1. Methods: Quantitative real time-PCR (QRT-PCR) was used to determine the miR-490-5p expression. Immunohistochemistry, qRT-PCR, and Western blot were employed to detect BUB1 and transforming growth factor-beta (TGFβ/Smad) signaling-related proteins expression in hepatic tissues and cells. The luciferase assay was used to confirm the targeting relationship between miR-490-5p and BUB1. The Cell Counting Kit-8, colony formation, Transwell invasion, scratch healing assays, and flow cytometry analysis were conducted to evaluate HCC cells proliferation, invasion, migration, and apoptosis alteration after transfection. Results: In HCC tissues and cells, lower expression of miR-490-5p was detected, while BUB1 was overexpressed than controls. The upregulation of miR-490-5p inhibited BUB1 expression and the overexpression of miR-490-5p or the under-expression of BUB1 inhibited HCC cells proliferation, migration, invasion, and increased the apoptosis rate. Conclusion: MiR-490-5p could regulate TGFβ/Smad signaling pathways by inhibiting BUB1, which could then inhibit HCC cells proliferation, invasion, and migration as well as decrease cell viability and increase apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells

Liu

et al. 2017

Pharmacology

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“…Real-time PCR revealed that miR-490-5p expression was significantly down-regulated in human bladder cancer tissues and cells, compared to normal adjacent bladder tissues and non-malignant bladder cell lines [30][31][32]. There was also an inverse correlation between the level of miR-490-5p and the pathological grade of bladder cancer [30].…”

Section: Discussionmentioning

confidence: 96%

Comprehensive investigation of aberrant microRNA profiling in bladder cancer tissues

Wei

et al. 2016

Tumor Biol.

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There has been accumulative evidence that microRNAs (miRNAs) play essential roles in the tumorigenesis and progression of bladder cancer. However, individual studies and small sample size caused discrepant outcomes. Thus, the current study focused on a comprehensive profiling of all differentially expressed miRNAs in a total of 519 bladder cancer tissue samples, based on miRNA microarray data. Altogether, 11 prioritized miRNAs stated by 21 published microarray datasets, including five down-regulated (miR-133a-3p, miR-1-3p, miR-99a-5p, miR-490-5p, and miR-133b) and six up-regulated candidate miRNAs (miR-182-5p, miR-935, miR-518e-3p, miR-573, miR-100-3p, and miR-3171) were analyzed with vote-counting strategy and a Robust Rank Aggregation method. Subsequently, miRNA in silico target prediction and potential pathway enrichment analysis were performed to investigate the prospective molecular mechanism of miRNAs in the tumorigenesis of bladder cancer. We found that most of the relative pathways of the aberrantly expressed miRNAs found in the current study were closely correlated with different biological processes, cellular components, molecular functions, cancer pathogeneses, and some cell signalings, such as Wnt signaling, insulin/IGF, PI3 kinase, and FGF signaling pathways. Hence, a comprehensive overview on the miRNA expression pattern in bladder cancer tissues was gained by the current study. These miRNAs might be involved in the tumorigenesis and deterioration of bladder cancer.

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“…MicroRNAs are a class of small non-coding RNAs, which possess various functions involving the regulation of apoptosis, proliferation, and autophagy [3][4][5][6]. MiR-26b (gene ID: 31612) belongs to the miR-26 fam-ily.…”

Section: Introductionmentioning

confidence: 99%

Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer

Zhao

Chen

et al. 2019

aoms

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A b s t r a c tIntroduction: Gastric cancer is one of the most common cancers of the digestive system and is associated with high morbidity and mortality. The aim of this study was to investigate whether miR-26b is involved in the proliferation and resistance to paclitaxel chemotherapy in gastric cancer cells. Material and methods: The expression of miR-26b in gastric cancer cell lines was determined by quantitative real-time PCR. Bioinformatics software was used to predict potential target genes of miR-26b. Luciferase assay was used to verify the interactions between target genes and miR-26b. CDC6 protein expression was measured by Western blot. The proliferation and chemotherapy resistance were analyzed by MTT assay. Cell invasion was evaluated by Transwell assay. Results: MiR-26b was down-regulated in gastric cancer cell lines compared to normal control cells, and its expression in drug resistance cells was even lower (p < 0.05). CDC6 was identified as a potential target gene of miR-26b by using bioinformatics analysis software. The expression of CDC6 was inhibited by miR-26b both at RNA level, which was determined by luciferase assay, and at protein level, which was determined by Western blot (p < 0.05). Silencing CDC6 inhibited cell proliferation, invasion, and promoted apoptosis of gastric cancer cell lines, BGC823 and SGC7901 (p < 0.05). Moreover, CDC6 knockdown inhibited chemotherapy resistance to paclitaxel, IC50 to paclitaxel decreased from 153.17 ±0.49 μg/l to 39.81 ±0.28 μg/l (p < 0.05). Conclusions: miR-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in the gastric cancer cell line SGC7901.

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MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer

Cited by 39 publications

References 31 publications

MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells

MiR-490-5p Suppresses Cell Proliferation and Invasion by Targeting BUB1 in Hepatocellular Carcinoma Cells

Comprehensive investigation of aberrant microRNA profiling in bladder cancer tissues

Mir-26b inhibits growth and resistance to paclitaxel chemotherapy by silencing the CDC6 gene in gastric cancer

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