MicroRNA-490-5P Targets CCND1 to Suppress Cellular Proliferation in Glioma Cells and Tissue Through Cell Cycle Arrest

Zhao, Long; Tang, Xiaoping; Luo, Renguo; Duan, Jie; Wang, Yuanchuan; Yang, Baofeng

doi:10.2174/1567202615666180813130143

Cited by 15 publications

(12 citation statements)

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“…[16] In addition, the epigenetic negative regulation of CCND1 by microRNA-490 is the key to glioma, and it provides a new perspective for the diagnosis, treatment, prognosis, and further transformation of glioma. [17] In the present study, BSP showed no significant difference in methylation status of CCND1 promoter among cervical adenocarcinoma, cervical squamous cell carcinoma, and normal cervical tissues, being inconsistent with the results by Illumina (850K) or qRT-PCR. However, the methylation of CCND1 gene cannot be excluded from significant indicators of cervical adenocarcinoma, considering the small number of BSP samples and detection sites.…”

Section: Discussioncontrasting

confidence: 94%

“…[16] It is proposed that the methylation status of paired box1 and SOX1 may be a new molecular marker for colorectal cancer screening. [17] There was a significant correlation between the down-regulation of SOX1 expression and the methylation of SOX1 promoter in primary hepatocellular carcinoma, and the methylation rate is 57.30%, being significantly higher than that in chronic hepatitis and cirrhosis. It is also discovered that DNA methylation levels of junctional adhesion moleculeA 3, SOX1, Slit guidance ligand 2, TTT, and C13ORF18 genes are significantly correlated with the prognosis of cervical intraepithelial neoplasia and can be used as predictors of cervical intraepithelial neoplasia prognosis.…”

Section: Discussionmentioning

confidence: 99%

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Tumor-suppressor gene SOX1 is a methylation-specific expression gene in cervical adenocarcinoma

Yuan

Yao²,

Abulizi³

2019

Medicine

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The present study is to analyze the difference of gene methylation in early cervical adenocarcinoma and to find molecular markers for predicting the occurrence and development of cervical adenocarcinoma.A total of 15 cases of primary cervical adenocarcinoma and 10 cases of primary cervical squamous cell carcinoma at stages IB1 or IIA1 were included in the study. Infinium MethylationEPIC BeadChip (850K) was used to screen specifically expressed genes in cervical adenocarcinoma tissues. Bisulfite sequencing polymerase chain reaction (BSP) and quantitative real-time polymerase chain reaction (qRT-PCR) were used to verify the methylation levels in cervical adenocarcinoma, cervical squamous cell carcinoma, and normal cervical tissues.Sex determining region Y-box 1 (SOX1) and cyclin D1 (CCND1) genes participated in multiple signaling pathways, being the central nodes of gene regulatory networks. SOX1 gene, but not CCND1 gene, was a specifically methylated gene in cervical adenocarcinoma according to BSP. According to qRT-PCR, methylation level of SOX1 in cervical adenocarcinoma tissues is significantly different from that in cervical squamous cell carcinoma tissues or normal cervical tissues, and the methylation level of CCND1 in cervical adenocarcinoma tissues or cervical squamous cell carcinoma tissues is significantly different from that in normal cervical tissues.The present study demonstrates that tumor-suppressor gene SOX1 is a methylation-specific expression gene of cervical adenocarcinoma and is expected to become a specific molecular marker for the diagnosis of cervical adenocarcinoma. However, CCND1 gene was not proven to be a specific methylation expression gene in cervical adenocarcinoma in the present study.

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Section: Discussioncontrasting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Tumor-suppressor gene SOX1 is a methylation-specific expression gene in cervical adenocarcinoma

Yuan

Yao²,

Abulizi³

2019

Medicine

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“…11 Low expression of miR-497-5p enhanced growth inhibition and apoptosis of glioma cells induced by TMZ, 12 however, Zhao et al demonstrated that miR-497-5p expression decreased glioma cell growth and invasion both in vitro and in vivo through Wnt3a/c-Jun feedback regulation. 13 Different from glioma, the function of miR-497-5p in other tumors is growth inhibition. For example, miR-497-5p can target YAP1 to inhibit the proliferation, invasion and migration of thyroid papillary carcinoma and non-small cell lung cancer, 14,15 and induce cycle arrest and apoptosis of breast cancer, gastric cancer, lung cancer and cervical cancer by regulating the expression of Bcl-w, Bcl-2, CDK6 or Cyclin E1.…”

Section: Introductionmentioning

confidence: 99%

<p>MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4</p>

Chen

Wang

et al. 2019

OTT

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miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated. Methods: Bioinformatics was used to analyze the target genes of miR-497-5p. qRT-PCR and Western blot were used to analyze mRNA and protein expression, respectively. Dualluciferase reporter assay was used to analyze the direct binding between miR-497-5p and 3ʹ-untranslated region of CBX4. Cell viability was measured with MTT assay. Flow cytometry was performed to detect cell cycle distribution. Results: Here, using bioinformatics methods we firstly found that miR-497-5p regulated cervical carcinoma proliferation by targeting polycomb chromobox4 (CBX4). Expression of miR-497-5p in cervical carcinoma tissues was negatively correlated with CBX4. A binding region of miR-497-5p in 3ʹ-untranslated region of CBX4 was predicted. Further experiments confirmed that miR-497-5p directly targeted CBX4. Besides, RNA interference of CBX4 inhibited cervical cancer cell proliferation, arrested cells at S phase and reduced the expression of CDK2 and Cyclin A2 proteins. The use of miR-497-5p inhibitor compromised CBX4 interference RNAs induced cycle arrest of cervical cancer cells. Cells co-transfected with miR-497-5p inhibitors and CBX4 interference RNAs had a higher proliferation rate than CBX4 inference RNA-transfected cells. Conclusion: All together, the present study demonstrates that miR-497-5p inhibits cervical cancer cells proliferation by directly targeting CBX4.

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“…MiR‐490 has a negative correlation with CCAT1 in NSCLC tissues, which is consistent with the findings of Zhao et al . in their study on glioma 28 . Overexpression of miR‐490 impaired H1299 cell viability and migration.…”

Section: Discussionmentioning

confidence: 92%

Long non‐coding RNA CCAT1 sponges miR‐490 to enhance cell proliferation and migration of non‐small cell lung cancer

et al. 2020

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Background Non‐small cell lung cancer (NSCLC) accounts for 85% of lung cancer which is the most frequently diagnosed malignancy in China. Colon cancer associated transcript 1 (CCAT1) acts as an oncogene in enhancing tumor progression. However, the effects of CCAT1 in NSCLC remain unclear. The purpose of this study was to explore the role of CCAT1 in NSCLC. Methods Wound healing and transwell assays were performed to measure cell migration. RT‐qPCR was employed to calculate the mRNA level of CCAT1 and miR‐490. Results High expression of CCAT1 was observed in NSCLC tissues and cells, with low expression of miR‐490. CCAT1 promoted the proliferation and metastasis of H1299 and A549 cells, while miR‐490 had the opposite effect. CCAT1 could specifically bind to miR‐490 and regulate its expression. MiR‐490 partially reversed the inhibitory effect of CCAT1 on cell proliferation and metastasis. Conclusions The CCAT1/miR‐490 molecular axis has been shown to be important for the treatment of NSCLC.

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MicroRNA-490-5P Targets CCND1 to Suppress Cellular Proliferation in Glioma Cells and Tissue Through Cell Cycle Arrest

Abstract: Overall, these results showed that epigenetic regulation of CCND1 via miR-490 was essential to glioma and provide a new insight into glioma diagnosis, treatment, prognosis and further translational investigations.

Cited by 15 publications

References 0 publications

Tumor-suppressor gene SOX1 is a methylation-specific expression gene in cervical adenocarcinoma

Tumor-suppressor gene SOX1 is a methylation-specific expression gene in cervical adenocarcinoma

<p>MicroRNA-497-5p Induces Cell Cycle Arrest Of Cervical Cancer Cells In S Phase By Targeting CBX4</p>

Long non‐coding RNA CCAT1 sponges miR‐490 to enhance cell proliferation and migration of non‐small cell lung cancer

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