MicroRNA‑494 inhibits apoptosis of murine vascular smooth muscle cells in�vitro

Abstract

Apoptosis of vascular smooth muscle cells (VSMCs) is a process that regulates vessel remodeling in various cardiovascular diseases. The specific mechanisms that control VSMC apoptosis remain unclear. The present study aimed to investigate whether microRNA-494 (miR-494) is involved in regulating VSMC apoptosis and its underlying mechanisms. Cell death ELISA and terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assays were used to detect apoptosis of murine VSMCs following stimulation with tumor necrosis factor-α (TNF-α). The results indicated that TNF-α upregulated VSMC apoptosis in a dose-dependent manner. Microarray analysis was used to evaluate the expression profile of microRNAs following TNF-α stimulation in murine VSMCs. The expression of miR-494 was downregulated, whereas B-cell lymphoma-2-like 11 (BCL2L11) protein expression levels were upregulated in VSMCs following treatment with TNF-α. Luciferase reporter assays confirmed that BCL2L11 was a direct target of miR-494. Transfection with miR-494 mimics decreased VSMC apoptosis and downregulated BCL2L11 protein levels. Conversely, transfection with miR-494 inhibitors increased cell apoptosis and upregulated BCL2L11 protein levels, suggesting that miR-494 may function as an essential regulator of BCL2L11. The increase in apoptosis caused by miR-494 inhibitors was abolished in cells co-transfected with BCL2L11-targeting small interfering RNA. The findings of the present study revealed that miR-494 inhibited TNF-α-induced VSMC apoptosis by downregulating the expression of BCL2L11.

Key miRNAs associated with memory and learning disorder upon exposure to sevoflurane determined by RNA sequencing

“…Neural stem cells differentiated from ESCs are suggested to be involved in cognition impairment-related diseases in humans (41,42). miR-467a-3p has also previously been found to be involved in the apoptosis of vascular smooth muscle cells (43). The present study showed that miR-467a-3p was differentially expressed in the hippocampus tissues of mice exposed to sevoflurane, which indicated that the neural differentiation and proliferation were dysregulated upon sevoflurane exposure.…”

HIF1A/miR‐20a‐5p/TGFβ1 axis modulates adipose‐derived stem cells in a paracrine manner to affect the angiogenesis of human dermal microvascular endothelial cells

“…In the present study, hypoxia exposure induces significant increases in In addition to growth factors, miRNAs also exert a key effect on angiogenesis via modulating the proliferation, differentiation, apoptosis, migration, and vascularization of angiogenesis-associated cells that play an essential role in numerous physiological and pathological processes (Sun, Li, Lei, & Li, 2018). miRNAs affect the regulation of post-transcriptional genes via binding to mRNAs in their 3′-UTRs, thus inducing translation degradation or obstruction within various biological processes (Cui et al, 2019;Karreth et al, 2011;Liu et al, 2019;Lu et al, 2018;Lucas et al, 2017;Siomi & Siomi, 2010;Thamotharan et al, 2017 -17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b, and miR-92a, demonstrate a variety of biological effects on angiogenesis (Dews et al, 2006;Landskroner-Eiger et al, 2015). miR-92a and miR-20a can target vascular endothelial growth factor A (VEGFA) and integrin subunit alpha 5 to suppress angiogenesis (Landskroner-Eiger et al, 2015).…”

“…In addition to growth factors, miRNAs also exert a key effect on angiogenesis via modulating the proliferation, differentiation, apoptosis, migration, and vascularization of angiogenesis‐associated cells that play an essential role in numerous physiological and pathological processes (Sun, Li, Lei, & Li, ). miRNAs affect the regulation of post‐transcriptional genes via binding to mRNAs in their 3′‐UTRs, thus inducing translation degradation or obstruction within various biological processes (Cui et al, ; Karreth et al, ; Liu et al, ; Lu et al, ; Lucas et al, ; Siomi & Siomi, ; Thamotharan et al, ). Interestingly, several miRNAs have been reported to exert a dual function on angiogenesis, depending on the cell type and their downstream targets.…”

Discovery of mmu-lncRNA129814/hsa-lncRNA582795 as a potential biomarker and intervention target for ischemia reperfusion injury-induced AKI

“…Some studies demonstrated that miRNA-494 mediated human nucleus pulposus and liver cell apoptosis [34,35], by the contrast, other studies found that it had an opposite effect on cardiomyocyte and vascular smooth muscle cell apoptosis [36,37]. The effect of miRNA-494-3p on apoptosis was similar with that of miRNA-494 [38][39][40].…”