MicroRNA-506 suppresses tumor proliferation and metastasis in colon cancer by directly targeting the oncogene EZH2

Zhang, Yi; Lin, Changwei; Liao, Guoqing; Liu, Sheng; Ding, Jie; Tang, Fang; Wang, Zhenran; Liang, Xingsi; Li, Bo; Wei, Yangchao; Huang, Qi; Li, Xuan; Tang, Bo

doi:10.18632/oncotarget.5309

Cited by 67 publications

(66 citation statements)

References 40 publications

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“…Furthermore, prior studies have also investigated how SFRP1 deficiency is linked with malignancies, including kidney cancer, colorectal cancer and breast cancer [25][26][27]. In our study, the SFRP1 mRNA and protein expression levels were significantly lower in bladder cancer tissues and cells compared to those in non-cancerous tissues and normal cells, which is consistent with the study of Buim [28]. During the G2/M phase of the vector transfection of SFRP1 overexpression in J82 cells, the proliferation, invasion, and migration rates decreased significantly whereas the number of cell apoptosis substantially increased.…”

Section: Discussionsupporting

confidence: 91%

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MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

Shang

Yang

Shen

et al. 2017

Cell Physiol Biochem

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Key WordsMiR-1-3p • SFRP1 • bladder cancer Abstract Background: Bladder cancer is of compelling morbidity and mortality due to its high recurrence rate. Little development has been made in the last decades in the therapy methods. Thus, the mechanism of its growth and invasiveness involving novel molecular targets are needed. Objective: Our research objective is to confirm the hypothesis that miR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells. Methods: The expression levels of miR-1-3p and SFRP1 were evaluated using RT-qPCR in bladder cancer tissues and cells as well as in normal tissues and cells. J82 cell lines were selected as experiment subjects due to their low expression levels of miR-1-3p. Plasmids carrying miR-1-3p mimics, miR-1-3p inhibitors and SFRP1 were transfected into the J82 cell lines. Subsequently, the protein expression of SFRP1 was detected using Western Blot analysis, and cell proliferation, apoptosis, invasion and migration ability was measured using MTT, the flow cytometry, the Transwell test and wound healing assays, respectively. Results: Bladder cancer tissues and cells exhibited significant decrease in the expression of miR-1-3p and SFRP1 compared to normal tissues and cells, and human bladder cancer cell line J82 exhibited the most significant decrease in these expressions (P < 0.05). MiR-1-3p up-regulates SFRP1 expression in bladder cancer cells, and the over-expression of miR-1-3p can suppress the proliferation, invasion and migration ability of bladder cancer cells. This mechanism is similar to the effect of SFRP1 over-expression on bladder cancer cells. Conclusion: MiR-1-3p suppresses the proliferation, invasion and migration of bladder cancer cells by up-regulating SFRP1 expression.

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Section: Discussionsupporting

confidence: 91%

“…For example, miR-27a has been identified to inhibit SFRP1 expression in glioma [13,28]. However, no literature has reported the relationship between miR-1-3p and SFRP1.…”

Section: Discussionmentioning

confidence: 99%

MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

Shang

Yang

Shen

et al. 2017

Cell Physiol Biochem

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“…Although many therapeutic modalities including surgery, resection and chemotherapy have been established, the treatment of colon cancer is still far from ideal [2, 3]. Therefore, the development of novel, less toxic therapeutic agents is imperative to reducing the high mortality and morbidity rates associated with colon cancer [4–6]. …”

Section: Introductionmentioning

confidence: 99%

A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy

et al. 2016

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Interleukin-15 has been implicated as a promising cytokine for cancer immunotherapy, while folate receptor α (FRα) has been shown to be a potentially useful target for colon cancer therapy. Herein, we developed F-PLP/pIL15, a FRα-targeted lipoplex loading recombinant interleukin-15 plasmid (pIL15) and studied its antitumor effects in vivo using a CT26 colon cancer mouse model. Compared with control (normal saline) treatment, F-PLP/pIL15 significantly suppressed tumor growth in regard to tumor weight (P < 0.001) and reduced tumor nodule formation (P < 0.001). Moreover, when compared to other lipoplex-treated mice, F-PLP/pIL15-treated mice showed higher levels of IL15 secreted in the serum (P < 0.001) and ascites (P < 0.01). These results suggested that the targeted delivery of IL15 gene might be associated with its in vivo antitumor effects, which include inducing tumor cell apoptosis, inhibiting tumor proliferation and promoting the activation of immune cells such as T cells and natural killer cells. Furthermore, hematoxylin and eosin staining of vital organs following F-PLP/pIL15 treatment showed no detectable toxicity, thus indicating that intraperitoneal administration may be a viable route of delivery. Overall, these results suggest that F-PLP/pIL15 may serve as a potential targeting preparation for colon cancer therapy.

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“…miR-506 also suppressed the proliferation and invasion of gastric cancer cells by targeting Yap1 (39). Furthermore, miR-506 served a tumorsuppressive function in hepatocellular carcinoma (40), colon cancer (28), and oral squamous cell carcinoma (41) by targeting various oncogenes. In the present study, we demonstrated the critical role of miR-506 in cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

“…Bioinformatic research revealed that FOXQ1 has a putative binding site for miR-506 (Fig. 4A), a well-recognized tumor-suppressor miRNA (28,29). A dual-luciferase reporter assay was performed to verify whether or not FOXQ1 is a target gene of miR-506.…”

Section: Foxq1 Is a Potential Target Of Mir-506mentioning

confidence: 99%

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

Zhang

Yan

et al. 2016

Oncology Reports

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Abstract. MicroRNAs (miRNAs) play a pivotal role in cancer progression and development, representing novel therapeutic tools for cancer therapy. Forkhead box Q1 (FOXQ1) functions as an oncogene in various cancer types. However, the functional significance of FOXQ1 in cervical cancer remains unknown. In this study, we investigated the biological function of FOXQ1 in cervical cancer and tested whether or not FOXQ1 can be targeted and regulated by specific miRNAs. We found that FOXQ1 was highly expressed in cervical cancer cell lines. Knockdown of FOXQ1 by small interfering RNA (siRNA) significantly suppressed the proliferation and epithelial-mesenchymal transition (EMT) of cervical cancer cells. FOXQ1 was predicted as a target gene of microRNA-506 (miR-506), and this prediction was validated by dual-luciferase reporter assay. Quantitative real-time PCR and western blot analyses demonstrated that mRNA and protein expression was negatively regulated by miR-506. The expression of miR-506 was downregulated in cervical cancer tissues, and miR-506 expression was inversely correlated with FOXQ1 expression in cervical cancer. The overexpression of miR-506 dramatically suppressed the proliferation and EMT of cervical cancer cells that mimicked the suppression of FOXO1 siRNA. Furthermore, the restoration of FOXQ1 expression significantly reversed the inhibitory effect of miR-506. Overall, our study demonstrated that miR-506 inhibited the proliferation and EMT of cervical cancer cells by targeting FOXQ1 and provided evidence that the miR-506/FOXQ1 axis plays an important role in the pathogenesis of cervical cancer, representing potential molecular targets for the development of anticancer agents for cervical cancer treatment.

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MicroRNA-506 suppresses tumor proliferation and metastasis in colon cancer by directly targeting the oncogene EZH2

Cited by 67 publications

References 40 publications

MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

MiR-1-3p Suppresses the Proliferation, Invasion and Migration of Bladder Cancer Cells by Up-Regulating SFRP1 Expression

A folate receptor-targeted lipoplex delivering interleukin-15 gene for colon cancer immunotherapy

Suppression of forkhead box Q1 by microRNA-506 represses the proliferation and epithelial-mesenchymal transition of cervical cancer cells

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