MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma

Su, Zhengming; Chen, Duqun; Zhang, Enpu; Li, Yifan; Yu, Zuhu; Shi, Min; Jiang, Ziyu; Ni, Liangchao; Yang, Shangqi; Gui, Yaoting; Ye, Jiongxian; Lai, Yongqing

doi:10.3892/mmr.2015.3498

Cited by 29 publications

(32 citation statements)

References 23 publications

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“…Additionally, functional analyses demonstrated that hsa-mir-509–3p binds and negatively regulates the transcription of CD44 more strongly in C allele carriers than in T allele carriers13151617. These results support previous studies that have implicated hsa-mir-509–3p as a tumor suppressor gene in the development of human cancer293031.…”

Section: Discussionsupporting

confidence: 86%

Associations of five polymorphisms in the CD44 gene with cancer susceptibility in Asians

Wang

Chen

et al. 2016

Sci Rep

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CD44 polymorphisms have been previously associated with cancer risk. However, the results between independent studies were inconsistent. Here, a meta-analysis was performed to systematically evaluate associations between CD44 polymorphisms and cancer susceptibility. A comprehensive literature search conducted in PubMed, Embase, and Web of Science databases through August 10, 2016 yielded 11 eligible publications consisting of 5,788 cancer patients and 5,852 controls. Overall, odds ratios (OR) calculated with 95% confidence intervals (CI) identified a significant association between CD44 polymorphism rs13347 and cancer susceptibility under all genetic models. Additionally, the minor allele of polymorphism rs11821102 was associated with a decreased susceptibility to cancer in allele contrast, dominant, and heterozygous models, while no significant association was identified for polymorphisms rs10836347, rs713330, or rs1425802. Subgroup analysis by ethnicity revealed rs13347 was significantly associated with cancer susceptibility for Chinese but not for Indians. Linkage disequilibrium (LD) between different polymorphisms varied across diverse ethnic populations. In conclusion, the results indicate that CD44 polymorphism rs13347 acts as a risk factor for cancer, especially in Chinese, while the minor allele of polymorphism rs11821102 may be associated with a decreased susceptibility to cancer. Nevertheless, further studies on a larger population covering different ethnicities are warranted.

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Section: Discussionsupporting

confidence: 86%

Associations of five polymorphisms in the CD44 gene with cancer susceptibility in Asians

Wang

Chen

et al. 2016

Sci Rep

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“…Previous studies have revealed that miRNAs have unique expression profiles in different types of cancer or diseases at different stages, which indicates that miRNAs could be used as biomarkers for diagnosis and serve a significant role in the occurrence and development of tumors (21,22). In RCC, certain miRNAs have been described as either oncogenes or tumor suppressors based on the function and expression level of miRNAs.…”

Section: Discussionmentioning

confidence: 99%

miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma

Jin

Zeng

et al. 2017

Oncol Lett

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Abstract. Renal cell carcinoma (RCC) is the most common type of kidney cancer, and the prognosis of metastatic RCC remains poor with a high rate of recurrence and mortality. A previous study has revealed that microRNA (miRNA), which negatively regulates protein expression, serves a role of oncogene or tumor suppressor. The aim of the present study was to investigate the expression and function of miR-514a-3p in RCC. To detect the expression of miR-514a-3p in 32 paired RCC tissues, quantitative polymerase chain reaction (qPCR) was performed. The function of miR-514a-3p in the proliferation, mobility and apoptosis of RCC cells (786-O and ACHN) was assessed by MTT, CCK-8, cell scratch, Transwell, Hoechst 33342 staining and flow cytometry assay. The results of qPCR revealed that miR-514a-3p was significantly downregulated in RCC tissues compared with adjacent normal tissues. Upregulation of miR-514a-3p by transfection of mimics suppressed RCC cell proliferation, migration and invasion, and induced cell apoptosis. The results revealed that miR-514a-3p was significantly downregulated in RCC and may serve a role as tumor suppressor in RCC. Further studies are required, focusing on the possibility of using miR-514a-3p as a biomarker for RCC as well as the pathway of miR-514a-3p in RCC.

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“…miR-509-3p, a significant regulator of the MAP3K8 oncogene was shown to be down regulated in tumor cells [71]. Furthermore, downregulation of miR-509-3p was shown to be a tumor suppressor in tumor cells and associated with tumor cell invasion and migration [72].…”

Section: Discussionmentioning

confidence: 99%

SPARC overexpression alters microRNA expression profiles involved in tumor progression

2017

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Medulloblastoma is the most common malignant brain tumor in children. SPARC (secreted protein acidic and rich in cysteine), a multicellular non-structural glycoprotein is known to be involved in multiple processes in various cancers. Previously, we reported that SPARC expression significantly impairs medulloblastoma tumor growth in vitro and in vivo and also alters chemo sensitivity. MicroRNAs are a class of post-transcriptional gene regulators with critical functions in tumor progression. In addition, microRNA (miRNA) expression changes are also involved in chemo-resistance. Herein, we assessed microRNA (miRNA) profiling to identify the functional network and biological pathways altered in SPARC-overexpressed medulloblastoma cells. A total of 27 differentially expressed miRNAs were identified between the control and SPARC-overexpressed samples. Potential messenger RNA (mRNA) targets of the differentially expressed miRNA were identified using Ingenuity Pathway Analysis (IPA). Network-based functional analyses were performed on the available human protein interaction and miRNA-gene association data to highlight versatile miRNAs among the significantly deregulated miRNAs using the IPA, and the biological pathway analysis using the PANTHER web-based tool. We have identified six miRNAs (miR-125b1*, miR-146a-5p, miR-181a-5p, miR-204-5p, miR-219-5p and miR-509-3p) that are associated with SPARC sensitivity by comparison of miRNA expression patterns from the SPARC treated cells with the control cells. Furthermore, pathway enrichment analysis outline that these six microRNAs mainly belong to biological processes related to cancer related signaling pathways. Collectively, these studies have the potential to indicate novel biomarkers for treatment response and can also be applied to develop novel therapeutic treatment for medulloblastoma.

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MicroRNA-509-3p inhibits cancer cell proliferation and migration by targeting the mitogen-activated protein kinase kinase kinase 8 oncogene in renal cell carcinoma

Cited by 29 publications

References 23 publications

Associations of five polymorphisms in the CD44 gene with cancer susceptibility in Asians

Associations of five polymorphisms in the CD44 gene with cancer susceptibility in Asians

miR‑514a‑3p functions as a tumor suppressor in renal cell carcinoma

SPARC overexpression alters microRNA expression profiles involved in tumor progression

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