MicroRNA-520e suppresses growth of hepatoma cells by targeting the NF-κB-inducing kinase (NIK)

Zhang, S; Shan, Changliang; Kong, Guangyao; Du, Yumei; Ye, L; Zhang, X

doi:10.1038/onc.2011.523

Cited by 105 publications

(88 citation statements)

References 45 publications

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“…The expression of miR-520e showed a tendency for increased values in the urine of the invasive tumour group compared with the controls. The findings of Zhang et al (31) suggest that miR-520e may be a tumour-suppressor miRNA in hepatocellular carcinomas since NF-κB-inducing kinase is one of the direct target genes of miR-520e. Further studies with a large sample size will confirm this tendency.…”

Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

et al. 2013

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Abstract. Since differential expression of microRNAs (miRNAs) has been found to be highly associated with several types of cancer, the goal of the present study was to identify an miRNA fingerprint as a non-invasive diagnostic tool to detect urinary bladder cancer using the easily accessible samples of whole blood and urine. Blood and urine samples from 4 controls and from patients suffering from superficial and invasive bladder cancer were analyzed using miRNA microarray consisting of 754 human miRNAs from the Sanger database v14. Using RT-qPCR technique, 6 of the differentially expressed miRNAs were validated in the controls (20 blood, 19 urine samples) and patients with superficial (18 blood, 16 urine samples) or invasive (20 blood and urine samples each) tumours. Three blood miRNAs (miR-26b-5p, miR-144-5p, miR-374-5p) were found to be significantly upregulated in invasive bladder tumour patients (P<0.05) when compared to the control group. The expression of 2 miRNAs (miR-618, miR-1255b-5p) in the urine of patients with invasive tumours was significantly (P<0.05) increased in comparison to the control group. Blood miR-26b-5p detected the presence of invasive bladder tumours with 94% specificity and 65% sensitivity. The urine miR-1255b-5p reached 68% specificity and 85% sensitivity in the diagnosis of invasive tumours. This pilot study represents the first characterization of an miRNA profile for urinary bladder tumours in whole blood samples. In addition, it was shown that invasive bladder tumours could be identified by differentially expressed urine miRNAs. Further studies are needed to test the clinical usefulness for bladder cancer detection and surveillance.

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Section: Discussionmentioning

confidence: 99%

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

et al. 2013

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“…For mature miR-506 detection, total RNA was polyadenylated by poly(A) polymerase (Ambion, Austin, TX, USA) as described previously [8] . Reverse transcription was performed using poly(A)-tailed total RNA and reverse transcription primer with ImPro-II Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer's instructions.…”

Section: Cell Transfectionmentioning

confidence: 99%

“…Many studies have emphasized the causal links between miRNA deregulation and cancer development. Our laboratory has reported that miR-520e (or miR-205) suppresses the growth of hepatoma cells through the targeting of NF-kB-inducing kinase (NIK) mRNA (or hepatitis B virus X protein mRNA) [8,9] . Recently, it has been reported that miR-506 inhibits epithelial mesenchymal transition (EMT) in ovarian cancer and breast cancer [10,11] .…”

Section: Introductionmentioning

confidence: 99%

MiR-506 suppresses proliferation of hepatoma cells through targeting YAP mRNA 3′UTR

et al. 2014

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Aim: MiR-506 is a miRNA involved in carcinogenesis of several kinds of cancer. In this study, we explored whether miR-506 played a critical role in hepatocellular carcinoma (HCC). Methods: Twenty HCC and adjacent normal liver tissue samples were collected. Human hepatoma cell lines HepG2 and H7402 were used for in vitro studies. The expression of miR-506 and transcriptional co-activator YAP was examined using qRT-PCR. Western blot analysis was used to measure the expression of YAP and its target genes. Luciferase reporter gene assay was used to identify YAP as a target gene of miR-506. MTT and EdU assays were carried out for functional analysis. Results: The expression of miR-506 was significantly lower in HCC than in adjacent normal liver tissues. Bioinformatics analysis revealed that YAP mRNA might be one of the targets of miR-506, and miR-506 in HCC tissues was found to be negatively correlated with YAP (r=-0.605). In both HepG2 and H7402 cells, miR-506 dose-dependently down-regulated YAP and its target genes c-Myc and the connective tissue growth factor (CTGF). Luciferase reporter gene assays demonstrated that miR-506 targeted the wild type 3′UTR of YAP mRNA, but not a 3′UTR with a mutant seed site. Furthermore, miR-506 significantly inhibited the proliferation of HepG2 and H7402 cells, while anti-miR-506 enhanced the cell proliferation, which was blocked by YAP siRNA. Conclusion: MiR-506 suppresses the proliferation of hepatoma cells by targeting YAP mRNA.

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“…Previous studies have demonstrated that dysfunction of miRNAs contributes to breast cancer development (11,14). Zhang et al reported that microRNA-520e (miR-520e) was associated with hepatocarcinogenesis (19); however, the biological significance of miR-520e in breast cancer remains largely unknown. The present study demonstrated that miR-520e is upregulated in breast cancer tissues and promotes proliferation, invasion and migration, and inhibits the apoptosis of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

Long

et al. 2016

Oncology Letters

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Abstract. Previous studies have indicated that the deregulation of microRNAs contributes to tumorigenesis. Misregulation of microRNA-520e (miR-520e) has been observed in various types of cancer. However, the expression profile and biological function of miR-520e in breast cancer remains largely unknown. The present study demonstrated that miR-520e expression was significantly increased in breast cancer tissues compared with adjacent non-cancerous breast tissues in 21 patients, as revealed by reverse transcription-quantitative polymerase chain reaction. Furthermore, the proliferation capacity of breast cancer cells was markedly enhanced by the introduction of miR-520e in vitro using a cell counting kit-8 assay. The present study also revealed that the overexpression of miR-520e could suppress breast cancer cell apoptosis, revealed using Annexin V/propidium iodide double staining and flow cytometry analysis. In addition, the ectopic expression of miR-520e promoted the migration of breast cancer cells in vitro, as demonstrated by a Transwell assay. Overall, the findings of the present study highlight an important role for miR-520e in breast cancer development and in the molecular etiology of breast cancer, which indicates the potential application of miR-520e in cancer therapy. IntroductionPrevious studies have demonstrated that alterations in protein-coding genes are critical for the development of human cancer (1,2). Other studies have indicated that deregulation of non-coding genes, particularly microRNAs (miRNAs), also contribute to tumorigenesis (3-6).miRNAs belong to a class of phylogenetically conserved non-coding RNAs that regulate an abundance of cellular processes by binding to specific target messenger RNAs (7). Numerous studies indicate that miRNAs are important in various biological processes, including the cell cycle, development, cell proliferation, differentiation and apoptosis (8-12). More than half of the miRNA genes are located in chromosomes that have been observed to become amplified, deleted or translocated during the development of cancer (13). In addition, deregulation of specific miRNAs occurs in certain cancer types (14,15), and this has been suggested to be associated with the prognosis of cancer patients (16). Furthermore, a previous study proposed that miRNAs may act as oncogenes or tumor suppressor genes (6). These findings highlight the importance of miRNAs in cancer development and provide insight into the molecular mechanisms that cause tumorigenesis.Globally, breast cancer is the most common cause of cancer-associated mortality in women, and accounted for ~1.38 million novel cases and 458,000 mortalities in 2008 (17). As with other solid tumors, multiple genetic and epigenetic alterations in protein-coding genes have been observed in breast cancer (18). However, previous findings alone cannot explain the complexity of breast cancer. Previous studies have demonstrated that dysfunction of miRNAs contributes to breast cancer development (11,14). Zhang et al reported that microRNA-520e...

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MicroRNA-520e suppresses growth of hepatoma cells by targeting the NF-κB-inducing kinase (NIK)

Cited by 105 publications

References 45 publications

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

Identification of microRNAs in blood and urine as tumour markers for the detection of urinary bladder cancer

MiR-506 suppresses proliferation of hepatoma cells through targeting YAP mRNA 3′UTR

miR-520e regulates cell proliferation, apoptosis and migration in breast cancer

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