MicroRNA-573 inhibits cell proliferation, migration and invasion and is downregulated by PICSAR in cutaneous squamous cell carcinoma

Abstract

The incidence of cutaneous squamous cell carcinoma (cSCC) has been increasing in recent years. Meanwhile, microRNAs (miRNAs) have been found to play vital roles in various cancers, including cSCC. This study aimed to investigate the expression of microRNA-573 (miR-573) in cSCC, its relationship with long non-coding RNA PICSAR and analyze its biological role. The relationship between PICSAR and miR-573 was confirmed by dual-luciferase reporter assay and Pearson’s correlation coefficient analysis. The levels of PICSAR and miR-573 were measured using quantitative Real-Time PCR. Cell Counting Kit-8 assay was used to evaluate the cSCC cell proliferation ability. The migration and invasion abilities of cSCC cells were evaluated by Transwell assay. PICSAR expression was increased and miR-573 was decreased in tumor tissues and cSCC cell lines. PICSAR and miR-573 can bind directly, and miR-573 expression was downregulated by PICSAR in cSCC. Overexpression of miR-573 significantly inhibited the proliferation, migration and invasion abilities of A431 and SCC13 cells. Additionally, miR-573 overexpression reversed the promotion effects of PICSAR overexpression on cSCC cell proliferation, migration and invasion abilities. In conclusion, our findings indicated that miR-573 expression was decreased in tumor tissues and cSCC cells and was downregulated by PICSAR in cSCC. Additionally, miR-573 overexpression inhibited cSCC cell proliferation, migration and invasion, and reversed the promotion effects of PICSAR overexpression on cSCC cell biological functions. Thus, miR-573 might function as a tumor suppressor and might be involved in the regulatory effects of PICSAR on tumorigenesis in cSCC.

Transcriptome Analysis and the Prognostic Role of NUDC in Diffuse and Intestinal Gastric Cancer

“…14 NUDC has been reported to be increased in acute myeloid leukemia, acute lymphocytic leukemia, chronic myelogenous leukemia, lung cancer, melanoma, and prostate cancer. 15,17,25,26 In addition, NUDC is reported to increase the proliferation, migration and invasion of renal carcinoma cells. 17 In the TCGA database-associated stomach cancer dataset (TCGA-STAD), the mutation rate of NUDC was 3% (3/440), and the copy number variation (CNV) gain and loss rates were 1.16% (5/432) and 12.73% (55/432), respectively.…”

“…15,17,25,26 In addition, NUDC is reported to increase the proliferation, migration and invasion of renal carcinoma cells. 17 In the TCGA database-associated stomach cancer dataset (TCGA-STAD), the mutation rate of NUDC was 3% (3/440), and the copy number variation (CNV) gain and loss rates were 1.16% (5/432) and 12.73% (55/432), respectively. We found an NUDC variant in 2 of 3 samples of SRC-GC but no variants in 5 WD-GC samples.…”

“…12,13 NUDC overexpression has been reported in several cancers, but there has been little research on the influence of NUDC on stomach cancer. [14][15][16][17][18] We conducted a survival analysis and investigated the role of NUDC in gastric cancer patients.…”

Corilagin Induces ROS-mediated Apoptosis and Triggers Cell-Cycle Arrest at G0/G1 Stage in Osteosarcoma Cells

“…However, 66% of bone tumors were cancerous, 15% were benign, and 19% were not cancerous at all (Li et al, 2015). The most frequent benign tumor was a giant cell tumor, while the most frequent malignant tumor was OS, which is described by immature osteoid development and atypical osteoblastic disparity (Tang et al, 2015). OS displays bimodal age dissemination with a high peak in young adults and a minor peak in the elderly (Tang et al, 2008).…”

Retraction: MicroRNA-573 functions as a tumor suppressor and is downregulated by PICSAR in cutaneous squamous cell carcinoma

“…Retraction: MicroRNA-573 functions as a tumor suppressor and is downregulated by PICSAR in cutaneous squamous cell carcinoma [ 1 ]. This article has been withdrawn at the request of the authors.…”