MicroRNA‐598 acts as an inhibitor in retinoblastoma through targeting E2F1 and regulating AKT pathway

Abstract

Recently, microRNAs (miRNAs) receive more attention due to their role in the pathogenesis of malignancies. Retinoblastoma (RB) is the most serious and harmful malignant tumor in infants and young children with eye diseases, which often endangers the lives of children. This study was designed to determine how miR-598 is involved in RB progression. In this study, quantitative reverse transcription-polymerase chain reaction, Western blot, dual-luciferase reporter, Cell Counting Kit-8, and Transwell assays were adopted to detect miR-598 expression and function in RB. The decreased expression of miR-598 was identified in RB. Overexpression of miR-598 suppressed the viability and metastasis of RB cells. Further, E2F transcription factor 1 (E2F1) is verified as a direct target of miR-598. Furthermore, E2F1 recovered miR-598-mediated-inhibition of cell viability and metastasis in RB. In addition, miR-598 was found to promote cell apoptosis and inactivate the protein kinase B (AKT) pathway in RB. miR-598 suppressed RB cell viability and metastasis through inhibiting E2F1 and inactivating AKT pathway, which may provide a new perspective for RB treatment.

MicroRNA copy number alterations in the malignant transformation of pleomorphic adenoma to carcinoma ex pleomorphic adenoma

“…MiR‐320a, miR‐383, and miR‐598 have been described as having a tumor suppressor role, being negatively regulated in several malignant tumors. In tumors of the lung, stomach, liver, colorectal, pancreas, bone marrow, prostate, esophagus, testis, bones, ovarium, breast, pancreas, central nervous system, and head and neck, overexpression of these miRNAs led to several suppressive features including inhibition of proliferation, migration, invasion, and metastasis; induced apoptosis; improved prognosis and sensitivity to chemotherapy 37–66 . In our study, we observed an increase CN for the genes miR‐320a, miR‐383, and miR‐598 in PA, while residual PA and CXPA displayed a decrease in CN, showing a potential tumor suppressor function, consistent with existing literature findings.…”

miR-1258 Attenuates Tumorigenesis Through Targeting E2F1 to Inhibit PCNA and MMP2 Transcription in Glioblastoma

“…Abnormal overexpression of E2F1 has also been reported in glioma (16,38). Interestingly, there has been increasing attention that miRNAs post-transcriptionally regulate E2F1 expression, including miR-205-5p, miR-342-3p, miR-93, and miR-598 (39)(40)(41). To better comprehend the tumor-suppressive function of miR-1258 mechanistically, we identified E2F1 as one of the direct and functional targets for miR-1258 in GBM.…”

Analysis of Human microRNA Expression Profiling During Diquat-Induced Renal Proximal Tubular Epithelial Cell Injury

“…Existing studies have found that miRNAs play a significant role in various biological processes, including cell apoptosis. 29,30 Furthermore, miRNAs have diagnostic and therapeutic value in many fields, such as tumor suppression, inflammation, 30,31 For example, in prostate cancer cells, miR-1972 targeted the miRNAs LIM and SH3 protein 1 (LASP1) and negatively regulated its expression, while upregulating miR-1972 inhibited cell proliferation, migration, invasion, and tumor formation and enhanced apoptosis. 32 In stroke-induced immunodeficiency syndrome, the increased expression of miR-4443 induced monocyte dysfunction by targeting tumor necrosis factor receptor-associated factor 4 (TRAF4), which may function as a crucial mediator, but in Graves' disease, this process induces CD4+ T cell dysfunction by targeting TRAF4.…”