MicroRNA‑623 inhibits tumor progression and is a predictor of poor prognosis of breast cancer

Abstract

Dysregulated microRNAs (miRNAs) serve vital roles in the progression and prognosis of breast cancer. miR-623 has been reported to influence the progression of numerous other cancers, such as lung adenocarcinoma and hepatocellular carcinoma, however, its role in breast cancer remains unclear. In the present study, the mRNA expression of miR-623 was studied in 121 pairs of breast cancer and adjacent normal tissues and cultured cell lines by reverse-transcription quantitative PCR. The association between miR-623 expression and clinical characteristics or the overall survival rate of patients was investigated by the χ 2 test or Cox regression analysis, respectively. The role of miR-623 in cell proliferation, migration and invasion of breast cancer cells was evaluated by cell transfection to regulate miR-623 expression and the CCK8 and Transwell assays, respectively. miR-623 was downregulated in breast cancer tissues and cell lines compared with normal tissues and breast epithelial cell lines. The χ 2 test demonstrated that the downregulation of miR-623 was associated with the tumor node metastasis (TNM) stage of patients with breast cancer. miR-623 and TNM stage were considered as two independent prognostic factors for breast cancer. Additionally, cell proliferation, migration, and invasion of breast cancer cells were promoted by the downregulation of miR-623, while upregulation of miR-623 led to inhibition of the aforementioned processes. Downregulation of miR-623 in breast cancer is associated with the development of breast cancer and indicates a poor prognosis of patients. The downregulation of miR-623 promotes cell proliferation, migration and invasion of breast cancer. The findings of the present study indicate that miR-623 functions as a prognosis biomarker and a tumor suppressor in breast cancer, which provides a potential therapeutic target for patients with breast cancer.

miR-623 Targets Metalloproteinase-1 and Attenuates Extravasation of Brain Metastatic Triple-Negative Breast Cancer Cells

“…41 miR-623 was also shown to suppress invasion of BC in vitro. 42 In this study, we demonstrated that BM TNBC cells express lower levels of miR-623 compared to their parental cells. Silencing miR-623 in TNBC cells with low BM propensity induces MMP1 expression.…”

Construction of a miRNA-mRNA Network Related to Exosomes in Colon Cancer

“…Studies have shown that the role of hsa-miR-623 as a tumor suppressor has been confirmed, such as targeting the effect of XRCC5 to inhibit the proliferation and metastasis of cancer cells such as breast cancer and liver cancer [ 49 ], inhibit the proliferation of gastric cancer cells, and increase the drug sensitivity of 5-fluorouracil by targeting cyclin D1 [ 50 ]. At the same time, hsa-miR-623 may be used as a predictor of the poor prognosis of breast cancer [ 51 ]. What attracts our particular attention is that the interaction between hsa-miR-623 and colon cancer has never been reported and the exosomes, as the source of hsa-miR-623, have never been studied.…”

HIF‐1α‐Mediated miR‐623 Regulates Apoptosis and Inflammatory Responses of Nucleus Pulposus Induced by Oxidative Stress via Targeting TXNIP

“…All of which indicated that these NP tissues could be regarded as the normal controls. However, it is still necessary to determine that miR-623 expression in NP tissues were not affected by the spinal tumor microenvironment, as it has been reported that miR-623 is a tumor suppressor and its expression may change in tumor patients [ 28 – 30 ]. Finally, although the essential role of the HIF-1 α -miR-623-TXNIP pathway was observed in regulating apoptosis and inflammatory responses induced by oxidative stress in NP cells, it must be noted that the findings were collected from the experiment results within normal NP cells.…”