MicroRNA-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3 Signaling

Li, Dong; Chen, Shuaiyin; Zhang, Weiguo; Zhang, Chao; Sun, Tiantian; Du, Yue; Ding, Ronghua; Gao, Yanlei; Jin, Yuefei; Duan, Guangcai

doi:10.1038/s41423-020-0453-4

Cited by 10 publications

(8 citation statements)

References 15 publications

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“…More recently, Duan et al showed the level of miR-628-5p was increased after EV71 infection. miR-628-5p suppressed TRAF3, which mediates IRF3 and NF-κB activation, to further affect IFNβ production during EV71 infection 73 . Taken together, IFN induction is regulated in a virus- and cell type-specific manner and IFNβ expression in EV71 infection is complicated and tightly regulated.…”

Section: Discussionmentioning

confidence: 99%

IKKε isoform switching governs the immune response against EV71 infection

et al. 2021

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The reciprocal interactions between pathogens and hosts are complicated and profound. A comprehensive understanding of these interactions is essential for developing effective therapies against infectious diseases. Interferon responses induced upon virus infection are critical for establishing host antiviral innate immunity. Here, we provide a molecular mechanism wherein isoform switching of the host IKKε gene, an interferon-associated molecule, leads to alterations in IFN production during EV71 infection. We found that IKKε isoform 2 (IKKε v2) is upregulated while IKKε v1 is downregulated in EV71 infection. IKKε v2 interacts with IRF7 and promotes IRF7 activation through phosphorylation and translocation of IRF7 in the presence of ubiquitin, by which the expression of IFNβ and ISGs is elicited and virus propagation is attenuated. We also identified that IKKε v2 is activated via K63-linked ubiquitination. Our results suggest that host cells induce IKKε isoform switching and result in IFN production against EV71 infection. This finding highlights a gene regulatory mechanism in pathogen-host interactions and provides a potential strategy for establishing host first-line defense against pathogens.

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Section: Discussionmentioning

confidence: 99%

IKKε isoform switching governs the immune response against EV71 infection

et al. 2021

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“…However, this small RNA has been implicated in several functions associated with BPD, including mitochondrial metabolism, autophagy, and oxidative stress responses in a variety of tissues [ 58 , 59 ]. On the other hand, miR-628-5p has been implicated in developmental functions such as embryonal implantation, as well as mechanisms of immune modulation, viral infection, and cell proliferation [ 60 , 61 , 62 ]. One study identified the fibroblast growth factor receptor 2 (FGFR2), the receptor for FGF10, as a target of miR-628-5p in ovarian cancer cells [ 63 ].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants

et al. 2021

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Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth, arrested fetal lung development, and inflammation. The incidence of BPD remains on the rise as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of BPD severity. We collected tracheal aspirates (TAs) from preterm babies with mild/moderate (n = 8) and severe (n = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, false discovery rate (FDR) < 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD group when compared to the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extremely premature infants contain miRNA signatures associated with severe BPD. These may serve as potential biomarkers of disease severity in infants with BPD.

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“…However, this small RNA has been implicated in several functions associated with BPD, including mitochondrial metabolism, autophagy, and oxidative stress responses in a variety of tissues [49,50]. On the other hand, miR-628-5p has been implicated in developmental functions such as embryonal implantation, as well as mechanisms of immune modulation, viral infection, and cell proliferation [51][52][53]. One study identified the fibroblast growth factor receptor 2 (FGFR2), the receptor for FGF10, as a target of miR-628-5p in ovarian cancer cells [54].…”

Section: Discussionmentioning

confidence: 99%

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

Siddaiah¹,

Oji‐Mmuo²,

Montes³

et al. 2021

Preprint

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Bronchopulmonary dysplasia (BPD) is a form of chronic lung disease that develops in neonates as a consequence of preterm birth and arrested fetal lung development. The incidence of BPD remains on the rise, as a result of increasing survival of extremely preterm infants. Severe BPD contributes to significant health care costs and is associated with prolonged hospitalizations, respiratory infections, and neurodevelopmental deficits. In this study, we aimed to detect novel biomarkers of severe BPD. We collected tracheal aspirates (TA) from preterm babies with mild/moderate (n = 8) and severe (n = 17) BPD, and we profiled the expression of 1048 miRNAs using a PCR array. Associations with biological pathways were determined with the Ingenuity Pathway Analysis (IPA) software. We found 31 miRNAs differentially expressed between the two disease groups (2-fold change, FDR &lt; 0.05). Of these, 4 miRNAs displayed significantly higher expression levels, and 27 miRNAs had significantly lower expression levels in the severe BPD vs. the mild/moderate BPD group. IPA identified cell signaling and inflammation pathways associated with miRNA signatures. We conclude that TAs of extreme premature infants contain miRNA signatures associated with severe BPD. These signatures may serve as biomarkers of disease severity in infants with BPD.

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MicroRNA-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3 Signaling

Cited by 10 publications

References 15 publications

IKKε isoform switching governs the immune response against EV71 infection

IKKε isoform switching governs the immune response against EV71 infection

MicroRNA Signatures Associated with Bronchopulmonary Dysplasia Severity in Tracheal Aspirates of Preterm Infants

Tracheal Aspirate miRNA Signatures in Preterm Infants with Severe Bronchopulmonary Dysplasia

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