MicroRNA‑675‑3p promotes esophageal squamous cell cancer cell migration and invasion

Abstract

Esophageal cancer ranks fourth in cancer-associated mortality in China and the incidence of esophageal adenocarcinoma has risen dramatically over the past two decades. MicroRNA (miRNA/miR) serves a pivotal role in human cancer cell growth, invasion and migration. MiR-675-3p is highly expressed in esophageal squamous cell cancer (ESCC) tissues, and may have an influence on ESCC cell migration and invasion. ESCC tumor tissue samples from 35 patients were profiled. MiR-675-3p expression was confirmed by reverse transcription-quantitative polymerase chain reaction. Manipulation of miR-675-3p via knockdown was carried out with subsequent evaluation of effects on cell proliferation, invasion, migration, and use of western blotting and ELISA assays. MiR-675-3p was overexpressed in ESCC tissues compared with normal tissues, and had higher expression levels in ESCC cells compared with the healthy esophageal epithelial cell line. The results revealed a predominant upregulation of cell migration and invasion ability. MiR-675-3p inhibitor inhibited ESCC cell proliferation, migration and invasion ability. It was also demonstrated that downregulation of miR-675-3p decreased the levels of matrix metalloproteinase (MMP) 2 and 9 and increased the level of E-cadherin. In addition, the effects of miR-675-3p inhibitor on ESCC cell lines were eliminated by con-transfection with miR-675-3p inhibitor and miR-675-3p mimic. In conclusion, the results indicated that miR-675-3p may be involved in the progression of ESCC through regulating ESCC cell migration and invasion capacity via modulating epithelial mesenchymal transition markers (MMP2, MMP 9 and E-cadherin).

ZFPM2-AS1 facilitates cell growth in esophageal squamous cell carcinoma via up-regulating TRAF4

“…MiRNAs could emerge as significant regulators in tumor initiation and progression [25]. Extensive reports have unveiled a series of dysregulated miRNAs in ESCC [26,27]. MiR-3612 is a novel miRNA that has not been studied in cancers.…”

Emerging Role of Non-Coding RNAs in Esophageal Squamous Cell Carcinoma

“…For example, miR-146a, miR-133b, miR-106b-3p, miR-219-5p, miR-206, miR-384, miR-455-5p, miR-128, miR-145-3p/5p, miR-10b-3p, miR-874-3p, miR-10a, miR-365, miR-301a, miR-6775-3p, miR-139-5p, miR-516b, miR-449a-5p, miR-125b, miR-433-3p, miR-370, miR-133b, miR-30a-3p/5p, miR-34a, miR-196a, and miR-125b-5p have been shown to act as tumor suppressors by inhibiting ESCC cell proliferation, promoting apoptosis by directly targeting oncogenes, or antagonizing pro-cancer signaling pathways [29,33,35,[40][41][42][43][44][45][46][47][48][49][50][51][52][54][55][56][57][58][60][61][62][63]110,133]. In contrast, pro-oncogenes such as miR-141, miR-21, miR-10b-3p, miR-424, miR-675-3p, miR-543, miR-135, miR-23b-3p, miR-502, miR-21-5p, and miR-548k have been reported to play contrasting roles in promoting cell proliferation or suppressing apoptosis in ESCC [47,[64][65][66][67][68][69][70][71][72][73]. Overall, the roles of miRNAs in cell proliferation and apoptosis can be crucial in ESCC pathogenesis.…”

<p>SNHG8 is upregulated in esophageal squamous cell carcinoma and directly sponges microRNA-411 to increase oncogenicity by upregulating KPNA2</p>

“…20 Extensive studies have shown that a plethora of miRNAs are dysregulated in ESCC, and the deregulated miRNAs are implicated in the pathogenesis and progression of ESCC by modulating multiple pathological processes. [21][22][23] Therefore, in-depth research into the regulatory roles of miRNAs in ESCC may provide novel opportunities for identifying effective techniques for the diagnosis and therapy of patients with ESCC.…”