MicroRNA‑6852 suppresses glioma A172 cell proliferation and invasion by targeting LEF1

Abstract

microRNA (miR)-6852 has been demonstrated to suppress the progression of gastric, colorectal and cervical cancer. The mechanism by which miR-6852 regulates glioma cells is yet to be elucidated. In the present study, reverse transcription-quantitative PCR analysis was used and the results demonstrated that miR-6852 expression was reduced in glioma tissues and cells. Cell counting kit-8 and transwell assay analysis indicated that proliferation, migration and invasion of A172 cells in the miR-6852 mimic group were lower than in the miR-NC group. Compared with the Inh-NC group, A172 cells of the Inh-miR-6852 group exhibited higher proliferation, migration and invasion. Additionally, the results indicated that lymphoid enhancer binding factor 1 (LEF1) was directly inhibited by miR-6852 and LEF1 expression was negatively correlated with miR-6852 expression in glioma tissues. Furthermore, the restoration of LEF1 reversed the effects of the miR-6852 mimics. The present findings suggested that miR-6852 inhibited glioma cells proliferation, migration and invasion by targeting the suppression of LEF1. Recently, miR-6852 has been demonstrated to be associated with the development of tumors, including those in gastric, colorectal and cervical cancer (9-11). The association of miR-6852 with glioma remains undetermined. Therefore, the present study aimed to assess miR-6852 expression in glioma, and further determine the effect of miR-6852 in the regulation of glioma progression. Materials and methodsGlioma tissue collection. A total of 32 pairs of glioma tissues (14 males and 18 females; age range, 31-56 years old; median age, 47 years old) and corresponding normal tissues were collected during biopsy from Affiliated Hospital of Hebei University (Hebei, China) between October 2014 and October 2016 for the current study. Normal tissues were derived from the temporal lobes and saddle area 2 cm away from tumor tissues. All glioma tissues were obtained from patients upon first diagnosis of glioma. Among these patients, 18 cases were high-grade and 14 cases were low-grade. All patients did not recieve radiotherapy or chemotherapy prior to surgery. Patients

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“…Wang et al found that the expression of LEF1 was negatively correlated with the expression of miR-6852. miR-6852 inhibited proliferation, migration, and invasion of glioma cells by inhibiting LEF1 (45). In our study, LEF1 was found to predict gastric cancer jointly with CDH3 and MMP7, which may be used as a diagnostic marker for gastric cancer in the future.…”

miR‑665 is downregulated in glioma and inhibits tumor cell proliferation, migration and invasion by targeting high mobility group box 1

“…Recent studies have shown that miRNA can exert an oncogenic or suppressor role in human cancer (36,37). For instance, miR-6852 expression is downregulated in glioma and suppresses cell proliferation, migration and invasion by targeting lymphoid enhancer-binding factor 1 (38). miR-454-3p exerts tumor-suppressive functions in glioblastoma, inhibiting cell proliferation and inducing apoptosis by targeting nuclear factor of activated T-cells, cytoplasmic 2 (39).…”

Screening and Identification of Key Biomarkers in Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

“…LEF1 encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1 [40]. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity.…”