microRNA‐802 inhibits cell proliferation and induces apoptosis in human cervical cancer by targeting serine/arginine‐rich splicing factor 9

Abstract

microRNAs (miRNAs) play crucial roles in cancer development and progression by targeting mRNAs for degradation and/or translational repression. micro-RNA-802 (miR-802) has been reported as a tumor suppressor and its deregulation is observed in various human cancers. However, the prognostic value of miR-802 and its underlying mechanisms involved in human cervical cancer are poorly investigated. The purposes of this study were to explore the role of miR-802 in cervical cancer and to clarify the regulation of serine/ arginine-rich splicing factor 9 (SRSF9) by miR-802. Here, we found that miR-802 was downregulated in both cervical cancer tissues and cell lines.Transfection of a miR-802 mimic into cervical cancer cells inhibited their proliferation and colony formation, and promoted cell cycle arrest at the G0/G1 phase and cell apoptosis. In addition, we found that miR-802 could directly target the 3′-untranslated region of SRSF9 and suppress SRSF9 expression. Rescue experiments revealed that overexpression of SRSF9 partially reversed the inhibition effect of miR-802 in cervical cancer cells. Overall, these findings demonstrate that miR-802 functions as a tumor suppressor in cervical cancer by targeting SRSF9, suggesting that miR-802 might serve as a potential therapeutic target in cervical cancer. K E Y W O R D S apoptosis, cervical cancer, microRNA-802, proliferation, serine/arginine-rich splicing factor 9How to cite this article: Zhang Q, Lv R, Guo W, Li X. microRNA-802 inhibits cell proliferation and induces apoptosis in human cervical cancer by targeting serine/arginine-rich splicing factor 9.

ZNF521 which is downregulated by miR-802 suppresses malignant progression of Hepatocellular Carcinoma through regulating Runx2 expression

“…miR-802 inhibits cell proliferation and induces apoptosis in human laryngeal cancer by targeting Camp-regulated phosphoprotein 19 [32]. These data further confirm that miR-802 exerted its effects was cell-specific [33,34]. Here, we demonstrated that miR-802 promoted HCC growth.…”

LncRNA IGFL2‐AS1 functions as a ceRNA in regulating ARPP19 through competitive binding to miR‐802 in gastric cancer

“…Zhang et al 34 reported that miR-802 promoted cell proliferation and inhibited cell apoptosis in human cervical cancer. 33 MiR-802 also suppressed non-small cell lung cancer development by regulating FGFR1. 34 Consistently, our findings suggested that overexpression of miR-802 suppressed cell growth, migration, and invasion in GC cells.…”

“…MiR‐802 has been demonstrated to be a tumor suppressor in multiple cancers. Zhang et al 34 reported that miR‐802 promoted cell proliferation and inhibited cell apoptosis in human cervical cancer 33 . MiR‐802 also suppressed non–small cell lung cancer development by regulating FGFR1 34 .…”

<p>MicroRNA-802 Suppresses Tumorigenesis of Colorectal Cancer via Regulating UBN2</p>

“…To date, large amount of evidence has showed that aberrant miR-802 expression is involved in the progression of multiple solid cancers. [11][12][13][14][15][16][17][18][19][20] Noteworthily, the biological role of miR-802 in the tumorigenesis of human cancer mainly depends on cancer type. Numerous studies reported that miR-802 functions as a tumor suppressor in most cancers, including glioblastoma, 12 tongue squamous cell carcinoma, 15 laryngeal, 17 breast, 18 gastric, 20 prostate, 13 ovarian, 16 and cervical cancer.…”

“…Numerous studies reported that miR-802 functions as a tumor suppressor in most cancers, including glioblastoma, 12 tongue squamous cell carcinoma, 15 laryngeal, 17 breast, 18 gastric, 20 prostate, 13 ovarian, 16 and cervical cancer. 19 Nevertheless, miR-802 was demonstrated to function as an onco-miRNA in lung carcinoma 14 and osteosarcoma. 11 Wang et al reported that miR-802 enhanced tumor cells proliferation via suppressing Menin in lung carcinoma; 14 Cao et al showed that miR-802 could promote the proliferation of osteosarcoma cells through regulating p27.…”