MicroRNA-873 (MiRNA-873) Inhibits Glioblastoma Tumorigenesis and Metastasis by Suppressing the Expression of IGF2BP1

Wang, Renjie; Li, Jianwei; Bao, Bin; Wu, Huancheng; Du, Zhenhua; Su, Jingliang; Zhang, Minghua; Liang, Haiqian

doi:10.1074/jbc.m114.624700

Cited by 90 publications

(73 citation statements)

References 37 publications

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“…However, it plays different roles in different tumors. miRNA-873 promotes the proliferation and migration of tumor cells by repressing Src in lung adenocarcinoma [14] but acts as a tumor suppressor in glioblastoma [13] and ovarian cancer [16]. However, little is known about the role of miRNA-873 in HCC.…”

Section: Discussionmentioning

confidence: 99%

“…miRNA-873 inhibits ER (estrogen receptor)-α activity and cell growth by targeting CDK3, mediating tamoxifen resistance [15]. miRNA-873 is downregulated in glioblastoma, inhibiting cell proliferation, migration and invasion and inducing apoptosis through suppressing the expression of IGF2BP1 [13]. However, it is upregulated in lung adenocarcinoma, acting as an oncogene.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, many miRNAs have been recognized to promote progression of HCC through enhancing oncogene expression or inhibiting tumor suppressor genes [10-12]. miRNA-873 has been reported to be frequently and aberrantly expressed in many cancers, such as glioblastoma [13], lung adenocarcinoma [14], breast cancer [15] and ovarian cancer [16]. miRNA-873 inhibits ER (estrogen receptor)-α activity and cell growth by targeting CDK3, mediating tamoxifen resistance [15].…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA-873 Promotes Cell Proliferation, Migration, and Invasion by Directly Targeting TSLC1 in Hepatocellular Carcinoma

Han¹,

Zhang²,

Ni³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and has the third highest mortality rate among all cancers. MicroRNAs are a class of endogenous, single-stranded short noncoding RNAs. The purpose of this study was to study the role of microRNA-873 in HCC. Methods: The expression of miRNA-873 and tumor suppressor in lung cancer 1 (TSLC1) in HCC tissues and cell lines was detected by real-time quantitative RT-PCR (RT-qPCR) or western blot. A CCK-8 assay was used to examine cell proliferation; flow cytometry was used to assess the cell cycle; the Transwell migration assay was used to test for metastasis. Luciferase assays were performed to assess whether TSLC1 was a novel target of miRNA-873. Results: We showed that miRNA-873 was upregulated in HCC tissues and cell lines compared with the normal control. Knockdown of miRNA-873 inhibited the growth and metastasis of HepG2 and accelerated G1 phase arrest, while overexpression of miRNA-873 had the opposite effect. The dual-luciferase reporter assays revealed that TSLC1 was a novel target of miRNA-873. Further study showed that TSLC1 was decreased in HCC tissues and cell lines. There was a negative correlation between the expression levels of TSLC1 and miRNA-873. The effect of miRNA-873 overexpression was neutralized by TSLC1. We also found that miRNA-873 activated the PI3K/AKT/mTOR signaling pathway and promoted HCC. Conclusions: Our data demonstrated that miRNA-873 promoted HCC progression by targeting TSLC1 and provided a new target for the therapy of HCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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MicroRNA-873 Promotes Cell Proliferation, Migration, and Invasion by Directly Targeting TSLC1 in Hepatocellular Carcinoma

Han¹,

Zhang²,

Ni³

et al. 2018

Cell Physiol Biochem

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“…Also an oncofetal protein, IGF2BP1 is commonly detected in several tumors. Its effects upon growth are variable with speculation of both oncogenic as well as tumorsuppressive functionality (31,(44)(45)(46) based upon the cellular context of its expression. As shown here, overexpression of IGF2BP1 in the committed erythroblasts did not prevent their maturation or enucleation in ex vivo cultures.…”

Section: Bcl11a Is Posttranscriptionally Regulated In Igf2bp1 Overexpmentioning

confidence: 99%

IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts

Vasconcellos

Tumburu

Byrnes

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Here we investigated in primary human erythroid tissues a downstream element of the heterochronic let-7 miRNA pathway, the insulinlike growth factor 2 mRNA-binding protein 1 (IGF2BP1), for its potential to affect the hemoglobin profiles in human erythroblasts. Comparison of adult bone marrow to fetal liver lysates demonstrated developmental silencing in IGF2BP1. Erythroid-specific overexpression of IGF2BP1 caused a nearly complete and pancellular reversal of the adult pattern of hemoglobin expression toward a more fetal-like phenotype. The reprogramming of hemoglobin expression was achieved at the transcriptional level by increased gamma-globin combined with decreased beta-globin transcripts resulting in gamma-globin rising to 90% of total beta-like mRNA. Delta-globin mRNA was reduced to barely detectable levels. Alpha-globin levels were not significantly changed. Fetal hemoglobin achieved levels of 68.6 ± 3.9% in the IGF2BP1 overexpression samples compared with 5.0 ± 1.8% in donor matched transduction controls. In part, these changes were mediated by reduced protein expression of the transcription factor BCL11A. mRNA stability and polysome studies suggest IGF2BP1 mediates posttranscriptional loss of BCL11A. These results suggest a mechanism for chronoregulation of fetal and adult hemoglobin expression in humans.IGF2BP1 | IMP1 | let-7 targets | fetal hemoglobin | ontogeny

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“…In a recent study, miR-873 was shown to be downregulated in breast tumors and to play a tumor suppressor role by targeting cyclin-dependent kinase 3, leading to the inhibition of ER-α activity and the suppression of breast cancer cell proliferation and tumor growth [31]. Insulin-like growth factor 2 mRNA binding protein 1 (IGFBP1) was identified as a target of miR-873 in glioblastoma [32]. IGFBP1, which is produced at high levels in the human endometrium, has been suggested as a marker of EP [33, 34].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-873 is a Potential Serum Biomarker for the Detection of Ectopic Pregnancy

Yan²,

Xu³

et al. 2017

Cell Physiol Biochem

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Background: Ectopic pregnancy (EP) refers to the implantation of the zygote outside the uterine cavity. In clinical practice, the diagnosis of EP relies on a combination of ultrasound findings and serum human chorionic gonadotrophin (hCG) measurements. However, the need for serial hCG measurements increases the risk of tubal rupture and death, underscoring the need to identify biomarkers for the early detection of EP. Methods: The serum concentrations of 21 microRNAs (miRNAs) associated with pregnancy or with known placental expression, as well as serum hCG and progesterone levels were analyzed 36 patients with viable intrauterine pregnancy (VIP), 30 patients with spontaneous abortion (SA), and 34 patients with EP using specific assay kits and reverse transcription PCR. The diagnostic performance of the different serum markers for detecting EP was analyzed by ROC curve analysis. Results: Five miRNAs were differentially expressed between the three groups, of which miR-873 and miR-223 were significantly lower in EP than in VIP and SA patients and did not change significantly according to gestational age, and miR-323 was significantly higher in EP than in VIP and SA. As a single marker, miR-873 had the highest sensitivity for detecting EP at 61.76% (at a fixed specificity of 90%). In comparison, the combination of hCG, progesterone and miR-873 had the highest sensitivity for detecting EP at 79.41% (at a fixed specificity of 90%). Conclusion: Although further validation in large-scale prospective studies is necessary, our results suggest that miR-873 could be a valuable noninvasive and stable biomarker for the early detection of EP.

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MicroRNA-873 (MiRNA-873) Inhibits Glioblastoma Tumorigenesis and Metastasis by Suppressing the Expression of IGF2BP1

Cited by 90 publications

References 37 publications

MicroRNA-873 Promotes Cell Proliferation, Migration, and Invasion by Directly Targeting TSLC1 in Hepatocellular Carcinoma

MicroRNA-873 Promotes Cell Proliferation, Migration, and Invasion by Directly Targeting TSLC1 in Hepatocellular Carcinoma

IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts

MicroRNA-873 is a Potential Serum Biomarker for the Detection of Ectopic Pregnancy

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