MicroRNA-92a-1-5p influences osteogenic differentiation of MC3T3-E1 cells by regulating β-catenin

Lin, Zhiping; Tang, Yangyang; Tan, Hongchang; Cai, Daozhang

doi:10.1007/s00774-018-0935-y

Cited by 12 publications

(10 citation statements)

References 44 publications

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“…MiR-92a-1-5p was a negative regulator of osteogenic differentiation. The expression of β-catenin was negatively regulated by miR-92a-1-5p, and the inhibition of miR-92a-1-5p on β-catenin was weakened by Wnt signal activator or GSK-3β knockout (Lin Z. et al, 2019). In addition, by regulating the expression of Wnt pathway related factors such as β -catenin, miR-26b-3p , miR-193a (Wang S. N. et al, 2018;, miR-4739 (Elsafadi et al, 2017), miR-150-3p (Wang N. et al, 2016), and miR-23a (Li et al, 2016) could also inhibit osteoblast differentiation but miR-101 (Wang H. et al, 2016) and miR-199b-5p (Zhao et al, 2016) could promote osteoblast differentiation.…”

Section: Wnt Pathwaymentioning

confidence: 98%

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

Zhao

2020

Front. Cell Dev. Biol.

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As an important pathological result of rheumatoid arthritis (RA), bone destruction will lead to joint injury and dysfunction. The imbalance of bone metabolism caused by increased osteoclast activities and decreased osteoblast activities is the main cause of bone destruction in RA. MicroRNAs (MiRNAs) play an important role in regulating bone metabolic network. Recent studies have shown that miRNAs play indispensable roles in the occurrence and development of bone-related diseases including RA. In this paper, the role of miRNAs in regulating bone destruction of RA in recent years, especially the differentiation and activities of osteoclast and osteoblast, is reviewed. Our results will not only help provide ideas for further studies on miRNAs' roles in regulating bone destruction, but give candidate targets for miRNAs-based drugs research in bone destruction therapy of RA as well.

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Section: Wnt Pathwaymentioning

confidence: 98%

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

Zhao

2020

Front. Cell Dev. Biol.

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“…17,18 miRNA-92a participates in the differentiation of osteoblast precursor cells into osteoblasts and can promote chondrogenesis. 19,28 In order to investigate the fracture healing and miRNA-92a-3p expression in patients with concomitant fractures and brain trauma, we collected samples from 30 patients (10 cases with completely healed fractures, 10 cases with isolated fractures, and 10 cases with concomitant fractures and brain trauma). The expression levels of miRNA-92a-3p in the plasma and callus were detected using RT-PCR.…”

Section: Resultsmentioning

confidence: 99%

miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

Liu

Xue

et al. 2021

Molecular Therapy - Nucleic Acids

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Patients who sustain concomitant fractures and traumatic brain injury (TBI) are known to have significantly quicker fracture-healing rates than patients with isolated fractures. The mechanisms underlying this phenomenon have yet to be identified. In the present study, we found that the upregulation of microRNA-92a-3p (miRNA-92a-3p) induced by TBI correlated with a decrease in integrin binding sialoprotein (IBSP) expression in callus formation. In vitro, overexpressing miRNA-92a-3p inhibited IBSP expression and accelerated osteoblast differentiation, whereas silencing of miRNA-92a-3p inhibited osteoblast activity. A decrease in IBSP facilitated osteoblast differentiation via the Phosphatidylinositol 3-kinase/threonine kinase 1 (PI3K/AKT) signaling pathway. Through luciferase assays, we found evidence that IBSP is a miRNA-92a-3p target gene that negatively regulates osteoblast differentiation. Moreover, the present study confirmed that pre-injection of agomiR-92a-3p leads to increased bone formation. Collectively, these results indicate that miRNA-92a-3p overexpression may be a key factor underlying the improved fracture healing observed in TBI patients. Upregulation of miRNA-92a-3p may therefore be a promising therapeutic strategy for promoting fracture healing and preventing nonunion.

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“…Experimental verification has shown that miR-92a-1-5p can negatively regulate β-catenin, a positive regulator of osteogenic differentiation. [ 35 ] There were also studies that the overexpression of miR-144-5p might reduce the viability of THP-1 macrophages by inhibiting the expression of Toll-like receptor 2 (TLR2) and inhibiting the activation of nuclear factor kappa-B (NK-κB) signaling and inhibit the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-8 in the cells. It was also proposed that miR-144-5p had the potential as a new therapeutic target for RA.…”

Section: Discussionmentioning

confidence: 99%

Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

Tang

Zhang

Dai

et al. 2021

Chinese Medical Journal

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Background: Circular RNA (circRNA) is a type of closed circular noncoding RNA (ncRNA), mostly formed by back-splicing or alternative splicing of pre-messenger RNA (mRNA). The aim of this study was to explore the expression profile of circRNA in peripheral blood mononuclear cells (PBMCs) of patients with ankylosing spondylitis (AS) and discover potential molecular markers of AS. Methods: The circRNA microarray technology was used to detect the expression of circRNAs in the peripheral blood of 6 patients with AS and 6 healthy controls (HC). To screen the differentially expressed circRNAs by fold change (FC) and P value, these differentially expressed circRNAs were analyzed by bioinformatics. In 60 cases of AS and 30 cases of HC, 4 circRNAs were subjected to real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), and their correlation with various clinical indicators was analyzed. Finally, the receiver operating characteristic (ROC) curve was used to analyze their potential as AS diagnostic markers. Results: The microarray results showed that there were 1369 significantly differently expressed ( P < 0.05, FC > 1.5) circRNAs between the AS and HC groups (675 upregulated and 694 downregulated). The results of bioinformatics analysis suggested that they were mainly involved in “enzyme binding,” “adenosine ribonucleotide binding,” “MAPK signaling pathway”, etc . The RT-qPCR results showed that the expressions of hsa_circRNA_001544 ( U = 486.5, P < 0.05) and hsa_circRNA_102532 ( U = 645, P < 0.05) were significantly different between the AS group and the HC group. The AS group was further divided into two subgroups: active AS (ASA) and stable AS (ASS). After analysis, it was found that compared with the HC group, hsa_circRNA_001544 was significantly increased in both ASA ( U = 214, P < 0.05) and ASS groups ( U = 273, P < 0.05), while hsa_circRNA_008961 ( U = 250, P < 0.05) and hsa_circRNA_102532 ( U = 295, P < 0.05) were only significantly increased in the ASA group. Furthermore, hsa_circRNA_012732 was significantly different between the ASA and ASS groups ( U = 194, P < 0.05), and there was no statistical significance among the remaining groups. Correlation analysis results showed that hsa_circRNA_012732 was negatively correlated with Bath Ankylosing Spondylitis Disease Activity Index (BASDAI), high-sensitivity C-reactive protein (hsCRP), and globulin (GLOB) and positively correlated with lymphocyte count (LY), mean corpusular volume, and albumin (ALB), and ...

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MicroRNA-92a-1-5p influences osteogenic differentiation of MC3T3-E1 cells by regulating β-catenin

Cited by 12 publications

References 44 publications

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

Roles of MicroRNAs in Bone Destruction of Rheumatoid Arthritis

miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition

Circular RNAs in peripheral blood mononuclear cells from ankylosing spondylitis

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