MicroRNA-92a Inhibits the Cell Viability and Metastasis of Prostate Cancer by Targeting SOX4

Abstract

MicroRNAs (miRNAs) was confirmed to play an active role in the pathogenesis of prostate cancer (PCa). The expression and biological function for miR-92a in PCa remains unknown. In this study, we demonstrated that miR-92a expression was decreased in PCa tissues and cells lines. Overexpression miR-92a inhibited the cell viability, migration and invasion of PC-3 while inhibition of miR-92a led to opposite alteration of cell viability and metastasis of DU-145 cells. Mechanically, we confirmed that miR-92a interacted with 3’-UTR of SOX4 through the complementary sequences by luciferase reporter assay. qRT-PCR and western blot confirmed that miR-92a inhibited the expression of SOX4 in PCa cells. Moreover, overexpression of SOX4 reversed the inhibitory effects of miR-92a overexpression on PC-3 cell viability, migration and invasion, while knockdown of SOX4 suppressed the promoting effects of miR-92a knockdown on these biological functions of DU-145 cells. Therefore, our study indicates that miR-92a inhibits the growth and metastasis of prostate cancer by targeting SOX4, and can potentially serve as a biomarker and treatment target for PCa patients.

Regulation of Neuroendocrine-like Differentiation in Prostate Cancer by Non-Coding RNAs

“…•  TP53 [229] mours of different origin [234] sion [242]   viability, migration, invasion [244]   proliferation [245] [247] MYCN [248]  PTEN…”

“…patients [199]   in expression concomitantly   proliferation and EMT [259] -- miR-363 biogenesis regulated by IFIT5, downstream of IFNgammaantiviral response) [189] in PCa [197][198][199] • [241]  TP53 [229]   in solid tumours of different origin [234] gration, invasion [242]   viability, migration, invasion [244]   proliferation [245] healthy [246] lncRNA FER1LR [247] hsa-miR-19b   in NEPC tissues [194] - MYCN [248]  PTEN…”

“…[241]  TP53 [229]   [242,243]   in solid tumours of different origin [234]   viability, migration, invasion [242]   viability, migration, invasion [244]   proliferation [245] - urine: PCa vs BPH vs healthy [246]  regulated by lncRNA FER1LR [247] hsa-miR-19b   in NEPC tissues [194] - MYCN [248]  PTEN…”

“… promotes PCa progression [270,271]   expression predicts poor survival [272]  blood: BPH vs PCa  urine: cancer cellmacrophage signalling [287]  urine: PCa vs healthy [288]  negative regulation by lncRNA TTTY15 [289]  suppresses AR via Myc [290] viability, migration, invasion [242] •…”

“…[241]  TP53 [229]   [242,243]   in solid tumours of different origin [234]   viability, migration, invasion [242]   viability, migration, invasion [244]   proliferation [245] -…”