MicroRNA-92a Promotes Colorectal Cancer Cell Growth and Migration by Inhibiting KLF4

Lv, Huiqing; Zhang, Zhongmin; Wang, Yaoxia; Li, Chenglin; Gong, Wu; Wang, Xin

doi:10.3727/096504016x14562725373833

Cited by 60 publications

(47 citation statements)

References 29 publications

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“…(also called gut-enriched Krüppel-like factor or GKL) 24 A number of miRNAs can bind to the 3′-untranslated region (3′-UTR) of KLF4 mRNA. [25][26][27][30][31][32][33][34] Hence, we predicted 15…”

Section: Discussionmentioning

confidence: 89%

Expression profiles of circular RNAs in human colorectal cancer based on RNA deep sequencing

Jin

et al. 2019

Clinical Laboratory Analysis

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BackgroundCircular RNAs (circRNAs) are a novel group of RNAs and play essential roles in cancers. However, the expression profiles of circRNAs in human colorectal cancer (CRC) are largely unclear.MethodsThe differentially expressed circRNAs, mRNAs, and microRNAs (miRNAs) between CRC tissues and paired adjacent normal tissues were first screened. Then, gene ontology and pathway analyses were performed to predict the possible functions. In addition, we identified the differentially expressed circRNAs in CRC correlated with Krüppel‐like factor 4 (KLF4) and validated their expression levels in CRC tissues. Finally, the correlations between hsa_circ_0142527 expression levels and clinicopathological features of patients with CRC were also analyzed.ResultsAfter filtered 4735 circRNAs by RNA deep sequencing, 67 differentially expressed circRNAs (fold change >2.0, P < 0.05) were selected. The top two pathways were cell cycle and other glycan degradation. Hsa_circ_0142527 and KLF4 mRNA were significantly lower expressed in CRC tissues in both training and confirm groups and have high positive correlation (r = 0.754). We further found that the expression levels of hsa_circ_0142527 were significantly associated with age (P = 0.004), differentiation (P = 0.008), invasion (P = 0.029), distal metastasis (P = 0.004), TNM stage (P = 0.005), and carcinoembryonic antigen (CEA; P = 0.037).ConclusionsThe circRNA expression profile of CRC provided new clues for understanding the occurrence of CRC. Hsa_circ_0142527 may be served as a potential biomarker for the diagnosis of CRC.

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Section: Discussionmentioning

confidence: 89%

Expression profiles of circular RNAs in human colorectal cancer based on RNA deep sequencing

Jin

et al. 2019

Clinical Laboratory Analysis

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“…Studies show that hypermethylation of CpG islands in the KLF4 promoter and methylation of histones modulate its activity in cancer and stem cells (9–12). Micro-RNAs (miR) are another mechanism that is important in modulating KLF4 expression in stem/progenitors cells, cardiovascular remodeling and during tumorigenesis (13–16). Multiple signaling pathways regulate the expression pattern of KLF4 via their effectors.…”

Section: Biochemical Activitiesmentioning

confidence: 99%

Krüppel-like factor 4 (KLF4): What we currently know

Ghaleb

Yang

2017

Gene

441

349

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Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor that regulates diverse cellular processes such as cell growth, proliferation, and differentiation. Since its discovery in 1996, KLF4 has been gaining a lot of attention, after it was shown in 2006 as one of four factors required for the induction of pluripotent stem cells (iPSCs). Here we review the current knowledge on the different functions and roles of KLF4 in various tissue and organ systems.

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“…Through regulating the protein expression of their target genes, miRs participate in various cellular biological processes, including cell proliferation, apoptosis, migration, invasion and tumorigenesis (6)(7)(8). In recent years, certain miRs have been implicated in the development and malignant progression of CSCC (9,10).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑34a directly targets high‑mobility group box 1 and inhibits the cancer cell proliferation, migration and invasion in cutaneous squamous cell carcinoma

Luo

Zhou

et al. 2017

Exp Ther Med

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Abstract. Cutaneous squamous cell carcinoma (CSCC) is the second most common type of skin cancer with increasing incidence. In recent years, several microRNAs (miRs) have been demonstrated to serve an oncogenic or tumor suppressive role in CSCC. However, the exact role of miR-34a in CSCC and the underlying regulatory mechanism remain unclear. The present study aimed to investigate the regulatory mechanism of miR-34a in the malignant phenotypes of CSCC cells using MTT assay, wound healing assay and transwell assay. It was observed that miR-34a was significantly downregulated in CSCC tissues and cell lines, and low miR-34a expression was associated with the aggressive progression of CSCC. Restoration of miR-34a significantly suppressed the proliferation, migration and invasion of CSCC SCL-1 cells. High-mobility group box 1 (HMGB1) was then identified as a target gene of miR-34a in SCL-1 cells using bioinformatics prediction. The expression of HMGB1 was significantly upregulated in the CSCC tissues and cell lines. Furthermore, the protein expression of HMGB1 was negatively regulated by miR-34a in SCL-1 cells, while overexpression of HMGB1 impaired the inhibitory effects of miR-34a on SCL-1 cells. These findings suggest that miR-34a represses the malignant phenotypes of CSCC cells, at least partly, via the inhibition of HMGB1. Therefore, miR-34a may be used as a promising therapeutic candidate for CSCC.

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MicroRNA-92a Promotes Colorectal Cancer Cell Growth and Migration by Inhibiting KLF4

Cited by 60 publications

References 29 publications

Expression profiles of circular RNAs in human colorectal cancer based on RNA deep sequencing

Expression profiles of circular RNAs in human colorectal cancer based on RNA deep sequencing

Krüppel-like factor 4 (KLF4): What we currently know

MicroRNA‑34a directly targets high‑mobility group box 1 and inhibits the cancer cell proliferation, migration and invasion in cutaneous squamous cell carcinoma

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