MicroRNA AND TUBERCULOSIS

Еремеев, В. В.; Evstifeev, Vladimir; Shepelkova, Galina; Эргешова, А.Э.; Багиров, М. А.

doi:10.15789/2220-7619-2018-3-309-315

Cited by 3 publications

(1 citation statement)

References 38 publications

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“…For early initiation of adequate chemotherapy, rapid differential diagnosis of the la-tent and active stages of the disease is important. MicroRNAs (miRNAs) might play a major part in the outcome of TB, because early research has implicated miRNAs as regulators of the immune response [ 7 , 8 , 9 , 10 ]. MiRNAs are small non-coding RNA molecules, capable of regulating a variety of biological processes, such as cell growth and differentiation, cell metabolism, immune response and inflammation.…”

Section: Introductionmentioning

confidence: 99%

MicroRNAs as Biomarkers of Active Pulmonary TB Course

et al. 2023

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The spread of drug-resistant forms of TB dictates the need for surgical treatment in the complex of anti-tuberculosis measures in Russia. Most often, surgical intervention is performed in the case of pulmonary tuberculoma or fibrotic cavitary tuberculosis (FCT). This study is devoted to the search for biomarkers that characterize the course of disease in surgical TB patients. It is assumed that such biomarkers will help the surgeon decide on the timing of the planned operation. A number of serum microRNAs, potential regulators of inflammation and fibrosis in TB, selected on the basis of PCR-Array analysis, were considered as biomarkers. Quantitative real time polymerase chain reaction and receiver operating curves (ROC) were used to verify Array data and to estimate the ability of microRNAs (miRNAs) to discriminate between healthy controls, tuberculoma patients, and FCT patients. The study showed that miR-155, miR-191 and miR-223 were differentially expressed in serum of tuberculoma with “decay” and tuberculoma without “decay” patients. Another combination (miR-26a, miR-191, miR-222 and miR-320) forms a set to differentiate between tuberculoma with “decay” and FCT. Patients with tuberculoma without “decay” diagnosis differ from those with FCT in serum expression of miR-26a, miR-155, miR-191, miR-222 and miR-223. Further investigations are required to evaluate these sets on a larger population so as to set cut-off values that could be applied in laboratory diagnosis.

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Section: Introductionmentioning

confidence: 99%

MicroRNAs as Biomarkers of Active Pulmonary TB Course

et al. 2023

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Composition of bacterial microbiome in sputum of patients with localized forms of pulmonary tuberculosis

Druzhinin,

Baranova,

Demenkov

et al. 2023

Pulʹmonologiâ (Mosk.)

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Recent studies have shown that the respiratory bacterial microbiome has an impact on the development of pulmonary tuberculosis. Changes in the composition of the microbiome have been associated with the pathogenesis of Mycobacterium tuberculosis infection, response to therapy, and clinical outcomes of the disease. To date, the composition of the respiratory microbiome has not been studied in patients with localized forms of pulmonary tuberculosis. Methods. In the present study, the taxonomic composition of the sputum microbiome of 14 patients with localized forms of pulmonary tuberculosis (tuberculomas) and 14 healthy donors in the comparison group was analyzed by sequencing (NGS) of the V3 – V4 region of the bacterial gene encoding 16S rRNA. Results. The sputum microbiomes of the patients and the control group did not have significant differences in the species richness index (Shannon). However, the patients showed a decrease in the uniformity index, another parameter of alpha diversity. Bacterial community structures (beta diversity) did not differ significantly between patients with localized forms of tuberculosis and healthy subjects. In patients with limited forms of tuberculosis, contrary to the decrease in the content of representatives of the phyla Fusobacteria, TM7, Tenericutes, Spirochaetes, and SR1, and of the genera Dialister, Mycoplasma, and Filifactor in the sputum, no clear dominance of any bacterial taxon was observed. Conclusion. Certain alpha and beta diversity parameters that characterize the sputum microbiome of patients with localized forms of pulmonary tuberculosis need to be confirmed in independent large-scale studies to further understand the role of the sputum microbiota in the development of localized forms of pulmonary tuberculosis. Determination of Prevotella titers in the sputum of these patients holds promise for the diagnosis of localized forms of pulmonary tuberculosis and the search for their genomic markers.

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Characteristics of Pulmonary Inflammation in Patients with Different Forms of Active Tuberculosis

Shepelkova,

Evstifeev,

Berezovskiy

et al. 2024

IJMS

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Targeted treatment of tuberculosis-associated lung damage requires an understanding of the precise mechanisms of immunopathology. A major obstacle to the longitudinal study of tuberculosis (TB) immunopathogenesis in humans is the lack of serial lung biopsies during disease progression and treatment, which could be used to characterize local immune pathways involved in tissue damage. Understanding of the immunobiology of lung tissue damage in tuberculosis has largely been based on animal models. Our study looked for signs of inflammation in TB patients’ lung biopsies. Results were compared between a site of infection and relatively healthy tissue outside the site. The most significant differences in the expression of microRNAs (miRs) and cytokine/chemokines were observed between the non-decayed tuberculoma and the surrounding parenchyma. In addition, these parameters showed almost no differences between the cavitary wall and surrounding tissue. This is an indication that the inflammatory process is more prevalent in fibrotic cavitary tuberculosis (FCT). In FCT subjects, no difference was observed between the cavity wall and the parenchyma in the production of key inflammatory factors such as IL-6, IL-11, IL-17, and IFNγ. This is an indication that the limits of the inflammatory response are broader in FCT. The expression levels of miR-191, miR-193a, miR-222, miR-223, miR-18, miR-155, miR-376c, miR-26a, miR-150, and miR-124 were not significantly different between the cavernous wall and lung tissue in patients with FCT, further confirming the spread of inflammatory and destructive processes beyond the focus of infection.

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MicroRNA AND TUBERCULOSIS

Cited by 3 publications

References 38 publications

MicroRNAs as Biomarkers of Active Pulmonary TB Course

MicroRNAs as Biomarkers of Active Pulmonary TB Course

Composition of bacterial microbiome in sputum of patients with localized forms of pulmonary tuberculosis

Characteristics of Pulmonary Inflammation in Patients with Different Forms of Active Tuberculosis

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