MicroRNA Biomarker hsa-miR-195-5p for Detecting the Risk of Lung Cancer

Abstract

Background. Lung cancer is one of the leading diagnosed cancers worldwide, and microRNAs could be used as biomarkers to diagnose lung cancer. hsa-miR-195 has been demonstrated to affect the prognosis of NSCLC (non-small-cell lung cancer) in a previous study. However, the diagnostic value of hsa-miR-195-5p in lung cancer has not been investigated. Methods. To evaluate the ability of hsa-miR-195-5p to diagnose lung cancer, we compared the expression of hsa-miR-195-5p in lung cancer patients, COPD patients, and normal controls. Receiver operating characteristic (ROC) curve analysis was performed to investigate the sensitivity and specificity of hsa-miR-195-5p. Coexpression network and pathway analysis were carried out to explore the mechanism. Results. We found that hsa-miR-195-5p had lower expression in lung cancer and COPD patients than in normal controls, and the AUC was 0.92 for diagnosing lung cancer. hsa-miR-143 correlated with hsa-miR-195-5p, and by combining these two microRNAs, the AUC was 0.97 for diagnosing lung cancer. Conclusions. hsa-miR-195-5p may act as a biomarker that contributes to the diagnosis of lung cancer and the detection of its high-risk population.

MiR-195-5p is a Potential Factor Responsible for CPNE1 Differential Expression between Subtypes of Non-Small Cell Lung Cancer

“…In ovarian cancer, overexpression of miR-195-5p can reduce cisplatin resistance and angiogenesis via PSAT1-depedent GSK3β/β-catenin signaling pathway [40]. To date, only four published papers have demonstrated the suppressive role of miR-195-5p in NSCLC cell proliferation and functioned as a predictor of poor prognosis [28][29][30][31]. In line with the published result, our further survival analysis proved that relatively low miR-195-5p expression was associated with unfavorable survival.…”

“…However limited studies have demonstrated the role of miR-195-5p in lung cancer. It is reported that miR-195-5p had lower expression in lung cancer and may act as a biomarker that contributed to the diagnosis of lung cancer and the detection of its high-risk population [28,29]. Moreover, miR-195-5p functions as a predictor of poor prognosis by directly targeting CIAPIN1 [30].…”

MicroRNAs as Potential Regulators of Immune Response Networks in Asthma and Chronic Obstructive Pulmonary Disease

“…Moreover, in blood, nine miRNAs including members of the miR-320 family, which target mitogen-activated protein kinase (MAPK) pathways, can be used for lung-cancer prediction in COPD subjects ( 98 ). Finally, the combined expression of hsa-miR-195 and hsa-miR-143, obtained from databases, is able to identify lung cancer compared to disease-free status, but cannot distinguish COPD from lung cancer ( 176 ). From peripheral leucocytes, differential expression of miR-106b-5p, miR-183-5p, miR-125a-5p, and miR-100-5p was found in COPD compared to healthy controls, and miR-106b-5p was directly correlated with disease severity alleviation ( 88 ).…”

Bioinformatics and system biology approach to identify the influences of SARS-CoV-2 infections to idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease patients

“…Gene-TFs and gene-miRNAs basically target main proteins to change the appearance in the progress of particular diseases. Hsa-mir-195-5p, hsa-mir-128-3p, and hsa-mir-129-2-3p target IL6 in these studies [ 74 , 82 ]. Furthermore, hsa-mir-941, hsa-mir-374a-5p, hsa-mir-17-3p, and hsa-mir-129-2-3p are targeted by the FLNC [ 82 , 83 ].…”