microRNA-Dependent Modulation of Genes Contributes to ESR1's Effect on ERα Positive Breast Cancer

Gao, Shan; Ding, Bo; Lou, Weiyang

doi:10.3389/fonc.2020.00753

Cited by 28 publications

(22 citation statements)

References 39 publications

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“…Therefore, further exploring the deep mechanism of PTC progression and searching for novel targets for PTC therapy are strongly needed. A number of miRNAs were found to be abnormally expressed in a broad spectrum of cancers, acting as an oncogene or tumor suppressor, and modulated cancer cell proliferation, apoptosis, migration, and invasion by negatively controlling the expression of their targets [ 2 , 7 ]. Some miRNAs even could be biomarkers for cancer diagnosis and prognostic, as well as therapeutic targets.…”

Section: Discussionmentioning

confidence: 99%

“…Since the first microRNA (miRNA) identified in 1993, research on miRNA biology has grown rapidly and accumulating miRNAs were discovered to play crucial roles in various biological processes, such as cell differentiation, proliferation, and survival [ 2 , 3 ]. In addition, aberrant miRNAs expression was found in numerous diseases, especially in cancer.…”

Section: Introductionmentioning

confidence: 99%

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MiR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3

Tang

et al. 2021

Bioscience Reports

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Background: The decreased level of miR-192-5p has been reported in several kinds of cancers, including bladder, colon, ovarian, and non-small cell lung cancer. However, the expression and function of miR-192-5p in papillary thyroid carcinoma (PTC) remains unknown. Objective: This study aimed to explore the function and underlying mechanism of miR-192-5p in PTC development. Methods: PTC tissues and relative normal controls from PTC patients were collected. qRT-PCR analysis was performed to measure miR-192-5p and SH3RF3 mRNA level in PTC tissues and cell lines. CCK-8 method and FCM assay were used to test cell proliferation and apoptosis in TPC-1 cells, respectively. The abilities of cell migration and invasion were detected by wound healing and transwell assays, respectively. The protein expression was evaluated by western blot. The interaction between miR-192-5p and SH3RF3 were confirmed by dual-luciferase reporter assay. Results: MiR-192-5p level was obviously decreased in PTC tissues and cell lines. Overexpression of miR-192-5p suppressed proliferation, migration, invasion, and EMT process, while induced apoptosis in TPC-1 cells. In addition, miR-192-5p negatively modulated SH3 domain containing ring finger 3 (SH3RF3) expression by binding to its 3’UTR. Silencing SH3RF3 inhibited the migration, invasion, and EMT of TPC-1 cells. At meantime, matrine, an alkaloid extracted from herb, exerted its anti-cancer effects in PTC cells dependent on increasing of miR-192-5p expression and decreasing of SH3RF3 expression. Conclusion: We firstly declared that miR-192-5p played a tumor suppressive role in PTC via targeting SH3RF3. Moreover, matrine exerted its anti-cancer effects in PTC via regulating miR-192-5p/SH3RF3 pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MiR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3

Tang

et al. 2021

Bioscience Reports

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“…It has been well documented that ncRNAs, including miRNAs, lncRNAs, and circular RNAs (circRNAs), participated in regulation of gene expression by talking with each other through the ceRNA mechanism. [18][19][20][21][22] To explore the upstream regulatory miRNAs of SEMA3F, we introduced seven prediction programs, involving PITA, RNA22, miRmap, microT, miRanda, PicTar, and TargetScan, to predict possible miRNAs that could potentially bind to SEMA3F. At the end, 13 miRNAs were finally obtained.…”

Section: Discussionmentioning

confidence: 99%

ncRNAs-mediated high expression of SEMA3F correlates with poor prognosis and tumor immune infiltration of hepatocellular carcinoma

Lou

Wang

Chen

et al. 2021

Molecular Therapy - Nucleic Acids

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Hepatocellular carcinoma (HCC) is notorious for its poor prognosis. Increasing evidence has demonstrated that semaphorin 3F (SEMA3F) plays key roles in initiation and progression of several types of human cancer. However, the specific role and mechanism of SEMA3F in HCC remains not fully determined. In this study, we first performed pan-cancer analysis for SEMA3F's expression and prognosis using The Cancer Genome Atlas (TCGA) and The Genotype-Tissue Expression (GTEx) data and found that SEMA3F might be a potential oncogene in HCC. Subsequently, noncoding RNAs (ncRNAs) contributing to SEMA3F overexpression were identified by a combination of a series of in silico analyses, including expression analysis, correlation analysis, and survival analysis. Finally, the TMPO-AS1/SNHG16-let-7c-5p axis was identified as the most potential upstream ncRNA-related pathway of SEMA3F in HCC. Moreover, SEMA3F level was significantly positively associated with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression. Collectively, our findings elucidated that ncRNAs-mediated upregulation of SEMA3F correlated with poor prognosis and tumor immune infiltration in HCC.

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“…The R package “limma” (bioconductor) was performed to analyze the differentially expressed genes (DEGs) between the normal and high WSS groups ( Smyth et al, 2005 ). The DEGs were screening out according to the following two cutoff criteria: adjusted p -value <0.05 and absolute log2 [fold change (FC)] >1 ( Rougeot et al, 2019 ; Gao et al, 2020 ). We removed the target DEGs that had no annotated gene names.…”

Section: Methodsmentioning

confidence: 99%

Prediction of Mechanosensitive Genes in Vascular Endothelial Cells Under High Wall Shear Stress

et al. 2022

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Objective: The vulnerability of atherosclerotic plaques is among the leading cause of ischemic stroke. High wall shear stress (WSS) promotes the instability of atherosclerotic plaques by directly imparting mechanical stimuli, but the specific mechanisms remain unclear. We speculate that modulation of mechanosensitive genes may play a vital role in accelerating the development of plaques. The purpose of this study was to find mechanosensitive genes in vascular endothelial cells (ECs) through combining microarray data with bioinformatics technology and further explore the underlying dynamics–related mechanisms that cause the progression and destabilization of atherosclerotic plaques.Methods: Microarray data sets for human vascular ECs under high and normal WSS were retrieved from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified through the R language. The performance of enrichment analysis and protein–protein interaction (PPI) network presented the biological function and signaling pathways of the DEGs. Hub genes were identified based on the PPI network and validated by GEO data sets. Predicted transcription factor (TF) genes and miRNAs interaction with potential mechanosensitive genes were identified by NetworkAnalyst.Results: A total of 260 DEGs, 121 upregulated and 139 downregulated genes, were screened between high and normal WSS from GSE23289. A total of 10 hub genes and four cluster modules were filtered out based on the PPI network. The enrichment analysis showed that the biological functions of the hub genes were mainly involved in responses to unfolded protein and topologically incorrect protein, and t to endoplasmic reticulum stress. The significant pathways associated with the hub genes were those of protein processing in the endoplasmic reticulum, antigen processing, and presentation. Three out of the 10 hub genes, namely, activated transcription factor 3 (ATF3), heat shock protein family A (Hsp70) member 6 (HSPA6), and dual specificity phosphatase 1 (DUSP1, also known as CL100, HVH1, MKP-1, PTPN10), were verified in GSE13712. The expression of DUSP1 was higher in the senescent cell under high WSS than that of the young cell. The TF–miRNA–mechanosensitive gene coregulatory network was constructed.Conclusion: In this work, we identified three hub genes, ATF3, HSPA6, and DUSP1, as the potential mechanosensitive genes in the human blood vessels. DUSP1 was confirmed to be associated with the senescence of vascular ECs. Therefore, these three mechanosensitive genes may have emerged as potential novel targets for the prediction and prevention of ischemic stroke. Furthermore, the TF–miRNA–mechanosensitive genes coregulatory network reveals an underlying regulatory mechanism and the pathways to control disease progression.

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microRNA-Dependent Modulation of Genes Contributes to ESR1's Effect on ERα Positive Breast Cancer

Cited by 28 publications

References 39 publications

MiR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3

MiR-192-5p inhibits proliferation, migration, and invasion in papillary thyroid carcinoma cells by regulation of SH3RF3

ncRNAs-mediated high expression of SEMA3F correlates with poor prognosis and tumor immune infiltration of hepatocellular carcinoma

Prediction of Mechanosensitive Genes in Vascular Endothelial Cells Under High Wall Shear Stress

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