microRNA expression analysis in endometriotic serum treated mesenchymal stem cells

Abdel-Rasheed, Mazen; Eldeen, Ghada Nour; Mahmoud, Mohamed; ElHefnawi, Mahmoud; Abu-Shahba, Nourhan; Reda, Mohamed; Elsetohy, Khaled A.; Nabil, Michael; Elnoury, Amr; Taha, Tamer; Azmy, Osama

doi:10.17179/excli2017-101

Cited by 8 publications

(6 citation statements)

References 54 publications

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“…For miRNA target analysis, we used 2 miRNA target analysis tools for the differentially expressed miRNAs as previously described 101 : miRror, which is a combinatorial miRNA target prediction tool, and miRWalk, which provides the predicted and experimentally validated targets for each miRNA. Within miRWalk, we used the intersection of different prediction algorithms (mirWalk2.0, miRanda, PicTar2, RNA22v2, and Targetscan 6.2) to obtain the most probable targets and minimize false positives.…”

Section: Discussionmentioning

confidence: 99%

In Silico Analysis of MicroRNA Expression Data in Liver Cancer

et al. 2023

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Abnormal miRNA expression has been evidenced to be directly linked to HCC initiation and progression. This study was designed to detect possible prognostic, diagnostic, and/or therapeutic miRNAs for HCC using computational analysis of miRNAs expression. Methods: miRNA expression datasets meta-analysis was performed using the YM500v2 server to compare miRNA expression in normal and cancerous liver tissues. The most significant differentially regulated miRNAs in our study undergone target gene analysis using the mirWalk tool to obtain their validated and predicted targets. The combinatorial target prediction tool; miRror Suite was used to obtain the commonly regulated target genes. Functional enrichment analysis was performed on the resulting targets using the DAVID tool. A network was constructed based on interactions among microRNAs, their targets, and transcription factors. Hub nodes and gatekeepers were identified using network topological analysis. Further, we performed patient data survival analysis based on low and high expression of identified hubs and gatekeeper nodes, patients were stratified into low and high survival probability groups. Results: Using the meta-analysis option in the YM500v2 server, 34 miRNAs were found to be significantly differentially regulated ( P-value ⩽ .05); 5 miRNAs were down-regulated while 29 were up-regulated. The validated and predicted target genes for each miRNA, as well as the combinatorially predicted targets, were obtained. DAVID enrichment analysis resulted in several important cellular functions that are directly related to the main cancer hallmarks. Among these functions are focal adhesion, cell cycle, PI3K-Akt signaling, insulin signaling, Ras and MAPK signaling pathways. Several hub genes and gatekeepers were found that could serve as potential drug targets for hepatocellular carcinoma. POU2F1 and PPARA showed a significant difference between low and high survival probabilities ( P-value ⩽ .05) in HCC patients. Our study sheds light on important biomarker miRNAs for hepatocellular carcinoma along with their target genes and their regulated functions.

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Section: Discussionmentioning

confidence: 99%

In Silico Analysis of MicroRNA Expression Data in Liver Cancer

et al. 2023

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“…miR26a-5p was found to be downregulated in the blood of patients with endometriosis compare with controls ( 208 ), and in B-lymphocytes of patients with SS ( 213 ).…”

Section: Role Of Mirnas In the Co-occurrence And Pathophysiology Of E...mentioning

confidence: 96%

“…Thus, several studies have investigated the participation and contribution of miRNAs in endometriosis as well as SS etiopathogenesis ( 204 , 205 ). Notably, various miRNAs have been reported to have a similar expression profile in both diseases, including miR16 ( 203 , 206 - 210 ), miR18a-5p ( 203 , 211 , 212 ), miR19b-3p ( 208 , 213 - 215 ), miR26a-5p ( 208 , 209 , 213 ), miR30c-5p ( 59 , 208 , 209 ), miR122-3p ( 214 , 216 ), miR142 ( 217 , 218 ), miR146a-5p ( 217 , 219 , 220 ), miR155-5p ( 218 , 221 ), miR181a ( 222 - 225 ), miR200b ( 220 , 226 ), miR223-3p ( 203 , 213 ) and miR378a-3p ( 213 , 227 ) ( Table II ).…”

Section: Role Of Mirnas In the Co-occurrence And Pathophysiology Of E...mentioning

confidence: 99%

“…miR17-5p was reported to be downregulated in the serum and blood of female patients with endometriosis ( 162 , 208 ) as well as in the saliva of patients with SS ( 203 , 210 ). Investigation of the expression of this miRNA led to its use as a non-invasive diagnostic marker of endometriosis ( 162 ).…”

Section: Role Of Mirnas In the Co-occurrence And Pathophysiology Of E...mentioning

confidence: 99%

“…miR19b-3p is downregulated in the blood of patients with endometriosis compared with controls ( 208 ) and in B-lymphocytes of patients with SS ( 213 ). This miRNA is a member of a miRNA cluster that plays a pivotal role in cell proliferation and apoptosis but is often deregulated in immune-associated diseases ( 233 ).…”

Section: Role Of Mirnas In the Co-occurrence And Pathophysiology Of E...mentioning

confidence: 99%

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Association of endometriosis with Sjögren's syndrome: Genetic insights (Review)

Zervou,

Tarlatzis,

Grimbizis

et al. 2024

Int J Mol Med

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Patients with a history of endometriosis have an increased risk of developing various autoimmune diseases such as rheumatoid arthritis, ankylosing spondylitis, systemic lupus erythematosus, multiple sclerosis and celiac disease. There is a potential association between endometriosis and an increased susceptibility for Sjögren's syndrome (SS). SS is a common chronic, inflammatory, systemic, autoimmune, multifactorial disease of complex pathology, with genetic, epigenetic and environmental factors contributing to the development of this condition. It occurs in 0.5-1% of the population, is characterized by the presence of ocular dryness, lymphocytic infiltrations and contributes to neurological, gastrointestinal, vascular and dermatological manifestations. Endometriosis is an inflammatory, estrogen-dependent, multifactorial, heterogeneous gynecological disease, affecting ≤10% of reproductive-age women. It is characterized by the occurrence of endometrial tissue outside the uterine cavity, mainly in the pelvic cavity, and is associated with pelvic pain, dysmenorrhea, deep dyspareunia and either subfertility or infertility. It is still unclear whether SS appears as a secondary response to endometriosis, or it is developed due to any potential shared mechanisms of these conditions. The aim of the present review was to explore further the biological basis only of the co-occurrence of these disorders but not their association at clinical basis, focusing on the analysis of the partially shared genetic background between endometriosis and SS, and the clarification of the possible similarities in the underlying pathogenetic mechanisms and the relevant molecular pathways.

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