MicroRNA expression in osteoarthritis: a meta-analysis

Liu, Huachen; Yan, Lei; Li, Xiaoke; Li, Dijun; Wang, Guishan; Shen, Nanping; Li, Jiao Jiao; Wang, Bin

doi:10.1007/s10238-023-01063-8

Cited by 9 publications

(9 citation statements)

References 65 publications

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“…[17] . Moreover, numerous miRNAs might participate in the process of OA development via modulating target gene expression [18] . Nevertheless, whether miRNAs can be used as a target for OA treatment with herbal medicines remains unknown.…”

Section: Resultsmentioning

confidence: 99%

Bamboo Leaf Flavone Regulates microRNA-127-5p to Hinder Interleukin-1 Beta-induced Chondrocyte Damage

Bu,

Zheng

2024

IJPS

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Prior studies have reported that bamboo leaf flavone possess biological and pharmacological activities in mammals. Yet, the effect and possible mechanism of bamboo leaf flavone on interleukin-1 beta-induced chondrocyte damage is still unclear. Methods interleukin-1 beta was used to induce human chondrocytes to establish a cell injury model, followed by exposure to different doses of bamboo leaf flavone. Apoptosis rate was analyzed using flow cytometry. Superoxide dismutase and lactate dehydrogenase activities were assessed using a kit. Interleukin-6 and interleukin-10 levels were determined using enzyme-linked immunosorbent assay. Quantitative reverse transcription-polymerase chain reaction analyzed microRNA-127-5p content. MicroRNA-NC and microRNA-127-5p mimics were transfected into chondrocytes, followed by treatment with interleukin-1 beta for 24 h. Cleaved caspase-3 and cleaved caspase-9 protein expression were monitored using Western blot. After bamboo leaf flavone treatment, apoptosis rate, cleaved caspase-3 and cleaved caspase-9 protein levels, lactate dehydrogenase activity, and interleukin-6 level were decreased, while microRNA-127-5p expression, superoxide dismutase activity, and interleukin-10 level were increased in dose-dependent ways. Upregulated microRNA-127-5p hindered apoptosis rate, cleaved caspase-3 and cleaved caspase-9 protein levels, the activity of lactate dehydrogenase, and the level of interleukin-6, and increased superoxide dismutase activity and interleukin-10 level. MicroRNA-127-5p knockdown could antagonize the effects of bamboo leaf flavone on interleukin-1 beta-mediated chondrocyte apoptosis, oxidative stress and inflammation. Bamboo leaf flavone exposure could inhibit cell apoptosis, oxidative stress and inflammation by up-regulating microRNA-127-5p expression, thereby reducing interleukin-1 beta-induced chondrocyte damage.

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Section: Resultsmentioning

confidence: 99%

Bamboo Leaf Flavone Regulates microRNA-127-5p to Hinder Interleukin-1 Beta-induced Chondrocyte Damage

Bu,

Zheng

2024

IJPS

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“…regarding miR-146a expression, 5 and miRNA expression in OA. 28 First one, published in 2022, 5 analyzes the expression profile of miR-146a in various tissues of OA patients. They concluded that the expression level of miR-146a in cartilage tissue and peripheral blood mononuclear cells was significantly higher in OA patients than in healthy controls.…”

Section: Discussionmentioning

confidence: 99%

Indirect influence of microRNA‐146a on the association of IL‐6 and TNF‐α genetic polymorphisms with the increased risk of hip osteoarthritis

Tudor,

Baranasic,

Knezevic

et al. 2024

Journal Orthopaedic Research

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Primary osteoarthritis (POA) is a complex hereditary disease that involves the interplay between genetics and epigenetics. MicroRNA molecules play important roles in epigenetic mechanisms. MicroRNA‐146a (miR‐146a) is a negative regulator of the immune response in osteoarthritis (OA). So, variations in the miR‐146a gene could affect OA risk. The aim of this study was to investigate the relationships between single nucleotide polymorphisms (SNPs) in the miR‐146a, interleukin‐6 (IL‐6), Toll‐like receptor 10 (TLR10), and tumor necrosis factor‐alpha (TNFA) genes and the risk for development of advanced‐stage primary hip osteoarthritis (PHOA) and primary knee osteoarthritis (PKOA) in the Croatian population. A total of 609 POA patients and 656 healthy donors were genotyped for SNPs in the miR‐146a (rs2910164, G>C). Since we used same patients and controls as two studies before us, we already had information about IL‐6 (rs1800795, C>G), TLR10 (rs11096957, C>T), and TNFA (rs1800629, C>T) genotypes of our subjects. None of the differences were statistically significant comparing either allelic or genotypic frequencies of miR‐146a SNP rs2910164 (G>C) between the PHOA and PKOA patients and controls. However, we found a significant association with risk to PHOA for the combination of genotypes (stratified miR‐146a genotype with the IL‐6, and stratified miR‐146a genotype with the TNFA). In a multifactorial disease such as POA, we have shown the indirect relevance of a second modifying factor (miR‐146a), which apparently contributes to the overall risk of PHOA. There was no risk association with the PKOA, indicating that these two localities (hip and knee) might have different risk‐modifying factors.

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“…According to the study, miR-146a-5p and miR-34a-5p were the most elevated miRNAs, while miR-127-5p and miR-140-5p were the most suppressed miRNAs. In osteoarthritis, mesenchymal stem cells and transforming growth factor-β were discovered to be the primary downstream effectors regulated by these miRNAs ( Liu et al, 2023 ).…”

Section: Mirnas and Age-related Diseasesmentioning

confidence: 99%

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies

Salama,

Eissa,

Doghish

et al. 2024

Front. Aging

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MicroRNAs (miRNAs) are short RNA molecules that are not involved in coding for proteins. They have a significant function in regulating gene expression after the process of transcription. Their participation in several biological processes has rendered them appealing subjects for investigating age-related disorders. Increasing data indicates that miRNAs can be influenced by dietary variables, such as macronutrients, micronutrients, trace minerals, and nutraceuticals. This review examines the influence of dietary factors and nutraceuticals on the regulation of miRNA in relation to the process of aging. We examine the present comprehension of miRNA disruption in age-related illnesses and emphasize the possibility of dietary manipulation as a means of prevention or treatment. Consolidating animal and human research is essential to validate the significance of dietary miRNA control in living organisms, despite the abundance of information already provided by several studies. This review elucidates the complex interaction among miRNAs, nutrition, and aging, offering valuable insights into promising areas for further research and potential therapies for age-related disorders.

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MicroRNA expression in osteoarthritis: a meta-analysis

Cited by 9 publications

References 65 publications

Bamboo Leaf Flavone Regulates microRNA-127-5p to Hinder Interleukin-1 Beta-induced Chondrocyte Damage

Bamboo Leaf Flavone Regulates microRNA-127-5p to Hinder Interleukin-1 Beta-induced Chondrocyte Damage

Indirect influence of microRNA‐146a on the association of IL‐6 and TNF‐α genetic polymorphisms with the increased risk of hip osteoarthritis

Decoding the secrets of longevity: unraveling nutraceutical and miRNA-Mediated aging pathways and therapeutic strategies

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