MicroRNA expression profiles in head and neck cancer cell lines

Tran, Nham; McLean, Tessa; Zhang, Xiaoying; Zhao, Chuan; Thomson, J. Michael; O’Brien, Christopher J.; Rose, Barbara

doi:10.1016/j.bbrc.2007.03.201

Cited by 242 publications

(224 citation statements)

References 31 publications

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“…(10)(11)(12)(13) Expression profiles have been established for many different cancers and seem to be unique to each cancer. (13,14) In HNSCC, several profiles of miRNA expression using miRNA microarray analysis have been reported (20)(21)(22)(23) and we also showed miR-205 and miR-21 as specific markers for HNSCC in …”

Section: Discussionsupporting

confidence: 67%

Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma

et al. 2011

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Hepatocyte growth factor (HGF) is a multifunctional molecule that acts as mitogen, motogen, and ⁄ or morphogen in a variety of cells. MET, a specific receptor tyrosine kinase for HGF, is upregulated in various tumors including squamous cell carcinoma of the human head and neck (HNSCC), but how HGF affects the expression of downstream functional genes has not yet been elucidated in detail. In the present study, we examined the expression of micro-RNA (miRNA), non-coding small RNA that regulate cell proliferation and functions by interfering with the translation of target mRNA, with or without HGF stimulation in HNSCC cell line HSC3. Among several miRNAs, in which the expression was altered after HGF stimulation, we focused on miR-200c and miR-27b, both of which were drastically downregulated after HGF stimulation. Expression of ZEB1, a target mRNA for miR-200c, was upregulated 3 and 6 h after HGF stimulation, and that of E-cadherin, a downstream molecule of ZEB1, was downregulated 12 h after HGF stimulation. Expression of ST14 ⁄ matriptase, an enzyme for extracellular matrix (ECM) degradation and HGF activation and a target mRNA for miR-27b, was drastically upregulated in the protein level after HGF stimulation, although it was not statistically altered in the mRNA level. These results suggest that miR-200c and miR-27b downregulated by HGF might play an important role in epithelialmesenchymal transition mediated by ZEB1 ⁄ E-cadherin and ECM degradation and HGF autoactivation mediated by ST14 ⁄ matriptase, respectively. Altered expression of miRNA directly regulated by HGF might contribute enhanced progressive and invasive characteristics of HNSCC by regulating the translation of HGF-induced functional molecules. (Cancer Sci 2011; 102: 2164-2171 S quamous cell carcinoma (SCC) is a common malignant tumor in the head and neck and constitutes 90% of malignancies in these regions.(1,2) The incidence and mortality of head and neck SCC (HNSCC) is increasing despite intense efforts, and its 5-year survival rate is <50%.(2,3) Several growth factors are considered to contribute to the carcinogenesis and progression of HNSCC.(2) One of these factors, hepatocyte growth factor (HGF), is a multifunctional growth factor that acts as mitogen, motogen and ⁄ or morphogen in a variety of cells including squamous epithelial cells.(4-6) MET, a specific receptor tyrosine kinase for HGF, is upregulated in various tumors including human HNSCC, and its signal transduction to the nucleus induces the expression of genes involving the progressive and invasive characteristics of HNSCC.(5-7) However, how HGF affects downstream functional gene expression has not yet been elucidated in detail.MicroRNA (miRNA) are non-coding small RNA (21-25 nucleotides) that regulate post-transcriptional gene expression by interfering with the translation of target mRNA. (8,9) One miRNA might regulate the expression of several genes and over one-third of all protein-coding genes might be under translational control by miRNA. (9) MiRNA are involved in a variety ...

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Section: Discussionsupporting

confidence: 67%

Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma

et al. 2011

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“…Of particular relevance to the present study are observations that miR-205 is up-regulated in a variety of carcinomas (8,9,30,31,44,45). Our finding that elevated levels of miR-205 markedly reduce SHIP2 in aggressive SCC cell lines provides some insight into a potential role of miR-205 in SCCs.…”

Section: Discussionsupporting

confidence: 54%

MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia

Ryan

Getsios

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

251

248

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M icroRNAs (miRNAs) are small, 20-to 24-nucleotide, noncoding RNAs found in diverse organisms. In animals, most miRNAs mediate posttranscriptional silencing by binding with partial complementarity to the 3Ј UTR of the target mRNA (1, 2). These endogenous, silencing RNAs have been shown to play important roles in development and differentiation (3-6), cellular stress responses (7), and cancer (8-11).The role of miRNAs in stratified squamous epithelia remains poorly understood. Inactivation of Dicer in mouse skin caused hair follicles to evaginate into the epidermis rather than invaginating downward, thus forming cyst-like structures (12, 13). These results underscore the importance of miRNAs in the regulation of epidermal and follicular development. miRNAs have also been extensively profiled in the corneal epithelium and show expression patterns that are regionally restricted (14). For example, miR-184 was the most abundant miRNA in the corneal epithelium; however, it was conspicuously absent from the limbal epithelium, an area enriched in corneal epithelial stem cells (15)(16)(17)(18). In contrast, miR-205 is broadly expressed throughout all viable cell layers in nearly all stratified squamous epithelia including the corneal, limbal, and conjunctival epithelia of the eye (12, 14). Thus, the corneal epithelium is unique in that it exhibits distinct as well as overlapping expression of miR-184 and miR-205 (14).miRNAs have been predicted to regulate thousands of mammalian genes (19); however, few targets have been experimentally validated for the great majority of these miRNAs. With the exception of a recent demonstration that a p63-related family member is negatively regulated by miR-203 (20), little is known about stratified squamous epithelial miRNA targets. We report that miR-205 represses SH2-containing phosphoinositide 5Ј-phosphatase 2 (SHIP2). Our finding that miR-184 negatively modulates the activity of miR-205 to maintain SHIP2 levels is the first demonstration that a miRNA can interfere with another to ensure the expression of a target protein. We show (i) that SHIP2 levels can be modulated in a variety of epithelial cells using gain-and loss-of-function experiments with miR-184 and miR-205 and (ii) that manipulating SHIP2 levels through miRNAs diminishes Akt signaling leading to decreased keratinocyte survival. Finally, we find a reciprocal relationship between miR-205 and SHIP2 expression in squamous cell carcinoma (SCC) cell lines and suggest that miR-205 may be viewed as a tumor promoter in the context of SCCs.Results miR-205 Targets SHIP2. We found miR-205 in all squamous epithelium that we examined (14). We also reported that miR-184 and miR-205 are the most abundant miRNAs in corneal epithelium and that miR-184 expression was restricted to the corneal epithelium (14). Bioinformatic analysis suggested that, in humans, the SHIP2 (Inppl1) 3Ј UTR is a putative target of both miR-184 and miR-205 (21) and is the only gene with overlapping binding sites to these two miRNAs. To test this prediction (Fig. 1...

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“…MiR-21 has been confirmed to be overexpressed in many malignancies such as gastric cancer (Chan et al, 2008), pancreatic cancer (Roldo et al, 2006), hepatocellular cancer (Meng et al, 2007), colon cancer (Slaby et al, 2007), breast cancer (Iorio et al, 2005), prostate cancer (Volinia et al, 2006), brain tumor (Chan et al, 2005), cholangiocarcinoma (Meng et al, 2006), lung cancer (Markou et al, 2008), esophageal cancer (Feber et al, 2008), head and neck cancer (Tran et al, 2007), and ovarian cancer (Iorio et al, 2007). In addition, clinical significance of serum miR-21 expression in human cancers has been reported in recent investigations.…”

Section: Discussionmentioning

confidence: 99%

Identification of Serum MicroRNA-21 as a Biomarker for Early Detection and Prognosis in Human Epithelial Ovarian Cancer

Xu¹,

Xi²,

Ge³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Recent investigations have confirmed up-regulation of serum miR-21 and its diagnostic and prognostic value in several human malignancies. In this study, we examined serum miR-21 levels in epithelial ovarian cancer (EOC) patients, and explored its association with clinicopathological factors and prognosis. The results showed significantly higher serum miR-21 levels in EOC patients than in healthy controls. In addition, increased serum miR-21 expression was correlated with advanced FIGO stage, high tumor grade, and shortened overall survival. These findings indicate that serum miR-21 may serve as a novel diagnostic and prognostic marker, and be used as a therapeutic target for the treatment of EOC.

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MicroRNA expression profiles in head and neck cancer cell lines

Cited by 242 publications

References 31 publications

Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma

Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma

MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia

Identification of Serum MicroRNA-21 as a Biomarker for Early Detection and Prognosis in Human Epithelial Ovarian Cancer

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